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    • 5. 发明公开
    • 정소 또는 태반 추출물을 함유하는 지방세포 분화촉진제
    • 包含测试或PLACENTA提取物的ADIPOGENESIS刺激物
    • KR1020120074649A
    • 2012-07-06
    • KR1020100136550
    • 2010-12-28
    • 영남대학교 산학협력단
    • 최인호이동목이은주이기호전용필전태훈
    • C12N5/077
    • C12N5/0653C12N2500/60C12N2500/84C12N2506/1323
    • PURPOSE: An adipocyte differentiation-promoting agent containing testis or placental extract is provided to be used as a therapeutic agent for obesity and diabetes. CONSTITUTION: An adipocyte differentitaion-promoting agent contains testis extract and/or placental extract as an active ingredient. A method for preparing the testis extract or placental extract comprises: a step of adding extraction solvent to testis or placenta tissue and homogenizing the same; a step of filtering the homogenized material to remove remnants; a step of adding water to filtered liquid and separating a lower layer and supernatant; a step of volatilizing organic solvent from the separated lower layer; a step of adding an alcohol solution; a step of adding an alkali solution and double-boiling the same; a step of adjusting pH concentration using an acid solution; a step of adding ether and mixing to separate layers; and a step of collecting and purifying an ether layer.
    • 目的:提供含有睾丸或胎盘提取物的脂肪细胞分化促进剂,用作肥胖和糖尿病的治疗剂。 构成:脂肪细胞分化促进剂含有睾丸提取物和/或胎盘提取物作为活性成分。 制备睾丸提取物或胎盘提取物的方法包括:向睾丸或胎盘组织中加入提取溶剂并使其均质的步骤; 过滤均质材料以除去残留物的步骤; 向过滤液中加水并分离下层和上清液的步骤; 从分离的下层挥发有机溶剂的步骤; 加入醇溶液的步骤; 加入碱溶液并使其双沸腾的步骤; 使用酸溶液调节pH浓度的步骤; 加入乙醚并混合分离层的步骤; 以及收集和纯化醚层的步骤。
    • 10. 发明公开
    • 고체분말을 이용한 3차원 세포 배양방법
    • 使用可注射固体培养自体细胞的方法
    • KR1020040058818A
    • 2004-07-05
    • KR1020020085331
    • 2002-12-27
    • 메디칸(주)
    • 이희영
    • C12N5/071
    • C12N5/0653A61K35/12C12N5/0075C12N5/0655C12N2531/00C12N2533/90
    • PURPOSE: A method for culturing self-cell using injectable solid powder is provided, thereby reducing the production costs and production process of the culture materials, so that it can be cheaply used for skin beauty such as wrinkle removing. CONSTITUTION: The method for culturing self-cell using injectable solid powder comprises the steps of: mixing serum extracted from human, subcultured preadipocyte, chondroblast and osteoblast, and air bubble free solid powder with the size of 20-500 micrometer; inducing the cell adhesion on the solid powder, wherein the air bubble free solid powder is selected from treated human dermis tissue having no sponge structure, chitosan, PLGA(DL-lactic-co-glycolic acid), treated human muscle membrane tissue having no sponge structure, polytetrafluorethylene(PTFE) powder, high density polyethylene powder, hydroxyapatite powder, methacrylate and solid silicone powder.
    • 目的:提供使用可注射固体粉末培养自体细胞的方法,从而降低培养材料的生产成本和生产过程,从而可以廉价地用于皮肤美容如皱纹去除。 构成:使用可注射固体粉末培养自体细胞的方法包括以下步骤:将从人,传代培养的前脂肪细胞,软骨细胞和成骨细胞提取的血清和大小为20-500微米的无气泡固体粉末混合; 诱导细胞粘附在固体粉末上,其中无气泡固体粉末选自经处理的没有海绵结构的人真皮组织,壳聚糖,PLGA(DL-乳酸 - 共 - 乙醇酸),处理的没有海绵的人肌肉膜组织 结构,聚四氟乙烯(PTFE)粉末,高密度聚乙烯粉末,羟基磷灰石粉末,甲基丙烯酸酯和固体硅氧烷粉末。