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    • 2. 发明公开
    • 3차원 세포배양으로 수득한 조건 배지를 유효성분으로 포함하는 허혈성 질환 치료용 약학적 조성물
    • 用于治疗包含以三维细胞培养物作为活性成分获得的调节培养基的化学疾病的药物组合物
    • KR1020120104771A
    • 2012-09-24
    • KR1020110022382
    • 2011-03-14
    • 서울대학교산학협력단
    • 김병수방석호
    • C12N5/00A61K35/12A61K8/98
    • C12N5/0037A61K35/12C12N2502/03A61K8/981C12N2500/02
    • PURPOSE: A pharmaceutical composition containing a conditioned medium prepared by three dimensional culture is provided to regenerate blood vessel in ischemic vascular diseases and to effectively treat ischemic vascular diseases. CONSTITUTION: A pharmaceutical composition for treating ischemic diseases contains conditioned medium as an active ingredient, prepared by three-dimensional culture of adult stem cells using a culture medium. The adult stem cells are adipose-derived stem cells, umbilical cord blood-derived stem cells, or bone marrow stem cells. The adult stem cells are cultured in a hypoxic condition. The buffer solution is 4-(2-hydroxy ethyl)-1-piperazine ethane sulfonic acid(HEPES) buffer solution. A cosmetic composition for preventing or treating alopecia and wrinkling contains the conditioned medium as an active ingredient. [Reference numerals] (AA) Secretion of angiogenic factor per cell(pg/10^5 cells); (BB) Concentration of cells in medium(1x10^5 cells/ml); (CC) Concentration of growth factors in medium(pg/ml)
    • 目的:提供含有通过三维培养制备的条件培养基的药物组合物,以在缺血性血管疾病中再生血管并有效治疗缺血性血管疾病。 构成:用于治疗缺血性疾病的药物组合物含有作为活性成分的条件培养基,其通过使用培养基的成体干细胞的三维培养制备。 成体干细胞是脂肪来源的干细胞,脐带血干细胞或骨髓干细胞。 成体干细胞在缺氧条件下培养。 缓冲溶液为4-(2-羟基乙基)-1-哌嗪乙烷磺酸(HEPES)缓冲溶液。 用于预防或治疗脱发和皱纹的化妆品组合物含有条件培养基作为活性成分。 (附图标记)(AA)每个细胞的血管生成因子的分泌(pg / 10 ^ 5细胞); (BB)培养基中细胞浓度(1×10 5细胞/ ml); (CC)培养基中生长因子浓度(pg / ml)