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    • 7. 发明公开
    • 면역 시스템을 재생하는 방법
    • 用于再生免疫系统的方法
    • KR1020080048463A
    • 2008-06-02
    • KR1020087004442
    • 2006-07-27
    • 에덴 바이오테크 엘티디
    • 겐,미트첼,제이.로샨,라메쉬로샨,로민
    • C12N5/071C12N5/02
    • A61K35/26A61K35/48C12N5/0647C12N2501/115C12N2501/125C12N2501/235
    • An isolated and purified cell line of hematopoeitic stem cells (HSC) that are incapable of expressing the CCR5 receptor on the cell surface ("the CCR5 32 cells" are used to regenerate the immune system in a subject in need thereof and especially to treat a subject infected with human immunodeficiency virus (HIV). The method is carried out by transplanting CCR5 32 into the recipient subject. Because mature immune cells derived from CCR5 32 cells cannot express functional CCR5 receptors, they will be resistant to infection by HIV and other pathogens that use the CCR5 receptor to infect cells. An embodiment of the invention includes administration of a nutritional regimen to the patient that optimizes conditions for CCR5 32 cell transplantation. Another embodiment of the invention includes co-transplanting mesenchymal cells along with the HSC in order to enhance the growth and development of the transplanted HSC.
    • 不能在细胞表面上表达CCR5受体(“CCR532细胞”)的分离和纯化的造血干细胞(HSC)细胞系用于在有需要的受试者中再生免疫系统,特别是治疗 感染人体免疫缺陷病毒(HIV)的方法是通过将CCR5 32移植到受体对象中进行的,因为源自CCR532细胞的成熟免疫细胞不能表达功能性CCR5受体,它们将对HIV和其他病原体的感染具有抗性 本发明的一个实施方案包括向患者施用优化CCR532细胞移植条件的营养方案,本发明的另一个实施方案包括与HSC共同移植间充质细胞,以便 增强移植HSC的生长发育。
    • 9. 发明公开
    • 이식용 세포의 생산방법
    • 用于生产用于移植的细胞的方法
    • KR1020060124791A
    • 2006-12-05
    • KR1020067022303
    • 2002-04-12
    • (주)안트로젠
    • 리,아이크,더블유.리우,구이쩬햄프,제임스크로센트,제프리,디.
    • C12N5/07C12N5/071A61K35/12
    • C12N5/0657A61K35/12A61K2035/124C12N5/0691C12N5/0692C12N2500/38C12N2501/105C12N2501/115C12N2501/135C12N2501/15C12N2501/155C12N2501/165C12N2501/235C12N2501/39C12N2502/1329
    • Methods for producing cells for transplantation are provided to enhance cell engraftment and cell survival rate of stem cells, monitor differentiation induction of stem cells in vitro, and collect stem cells for transplantation. The method for producing cells for transplantation comprises the steps of: (a) supplying immortalized bone marrow stem cells; (b) culturing the immortalized bone marrow stem cells under conditions that induces the differentiation of the immortalized bone marrow stem cells into vascular smooth muscle cells, endothelial cells, epicardial cells, adipocytes, osteoclasts, osteoblasts, cardiac fibroblasts, macrophages, neuronal progenitors, neurons, astrocytes, skeletal muscle cells, smooth muscle cells, pancreatic precursor cells, pancreatic beta-cells or hepatocytes; (c) monitoring the differentiation state of the cells of step(b); and (d) collecting the cells of step(b) when 10-100% of cells express a detectable amount of proteins specific to the cell type, wherein the method further comprises the step of transplanting the cells into mammals.
    • 提供生产移植细胞的方法,以增强干细胞的细胞移植和细胞存活率,监测体外干细胞的分化诱导,并收集干细胞用于移植。 用于生产移植细胞的方法包括以下步骤:(a)供应永生化骨髓干细胞; (b)在诱导永生化骨髓干细胞分化为血管平滑肌细胞,内皮细胞,心外膜细胞,脂肪细胞,破骨细胞,成骨细胞,心脏成纤维细胞,巨噬细胞,神经元祖细胞,神经元的条件下培养永生化骨髓干细胞 ,星形胶质细胞,骨骼肌细胞,平滑肌细胞,胰腺前体细胞,胰腺β细胞或肝细胞; (c)监测步骤(b)的细胞的分化状态; 和(d)当10-100%的细胞表达可检测量的对细胞类型特异的蛋白质时,收集步骤(b)的细胞,其中所述方法还包括将细胞移植到哺乳动物中的步骤。