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    • 7. 发明授权
    • 저온유도성프로모터서열
    • KR100452853B1
    • 2005-01-27
    • KR1019970705863
    • 1996-12-26
    • 니뽄 다바코 산교 가부시키가이샤
    • 미네토시키오오야마아키오히요시토오루카사오카케이스케
    • C12N15/82C12Q1/68
    • C07K14/415C12N15/8226C12N15/8237
    • This invention discloses a novel cold-inducible promoter which induces gene expression at low temperatures in potato tubers but which is scarcely induced in organs other than tuber or at normal temperature, which induces gene expression for a long time not less than five months. The promoters of this invention are the DNA sequence having a nucleotide sequence from first to 3546th nucleotide in the nucleotide sequence shown in SEQ ID. No. 1, or a part thereof having a cold-inducible promoter activity, or a DNA sequence having the same nucleotide sequence as said DNA sequences except that one or more nucleotides are deleted or substituted, or one or more nucleotides are inserted or added, which DNA sequence has a cold-inducible promoter activity and the DNA sequence having a nucleotide sequence from first to 4120th nucleotide in the nucleotide sequence shown in SEQ ID. No. 2, or a part thereof having a cold-inducible promoter activity, or a DNA sequence having the same nucleotide sequence as the said DNA sequences except that one or more nucleotides are deleted or substituted, or one or more nucleotides are inserted or added, which DNA sequence has a cold-inducible promoter activity.
    • 本发明公开了一种新颖的低温诱导型启动子,其在马铃薯块茎低温诱导基因表达,但几乎不在除块茎或常温以外的器官中诱导,其诱导不少于五个月的长时间基因表达。 本发明的启动子是具有SEQ ID NO.1所示核苷酸序列中第一至第3546个核苷酸的核苷酸序列的DNA序列。 具有冷诱导型启动子活性的序列号1或其部分,或具有与所述DNA序列相同的核苷酸序列的DNA序列,除了一个或多个核苷酸缺失或被取代或插入或添加一个或多个核苷酸之外, 该DNA序列具有冷诱导型启动子活性,并且该DNA序列具有SEQ ID.NO.1所示核苷酸序列中第一至第4120位核苷酸的核苷酸序列。 编号2或其具有冷诱导型启动子活性的部分,或具有与所述DNA序列相同的核苷酸序列的DNA序列,除了一个或多个核苷酸缺失或被取代,或者一个或多个核苷酸被插入或添加 该DNA序列具有冷诱导型启动子活性。