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    • 1. 发明公开
    • 돼지 설사병 저항성 유전자 검정방법
    • 抗性基因检测方法与PORCINE EPIDEMIC DIARRHEA
    • KR1020020080504A
    • 2002-10-26
    • KR1020010019282
    • 2001-04-11
    • 최윤재이승호조광근
    • 최윤재조광근이승호김철욱
    • C12Q1/68
    • PURPOSE: Provided is a detection method of resistance gene against porcine epidemic diarrhea by examining resistance and susceptibility against Escherichia coli F18 receptor. CONSTITUTION: The detection method is characterized by the steps of: preparing primers for detecting mutation of FUT1 gene; and detecting a pig, which shows resistance against porcine epidemic diarrhea, by PCR using the prepared primers and a test sample collected from a pig. Wherein, the primers are selected from Int 1(SEQ ID NO:1), Int 2(SEQ ID NO:2), Ext 1(SEQ ID NO:3), Ext 2(SEQ ID NO:4) and Ext 2-2(SEQ ID NO:5); and PCR products are subjected to agarose gel electrophoresis.
    • 目的:通过检测对大肠杆菌F18受体的耐药性和易感性,提供了针对猪流行性腹泻的抗性基因的检测方法。 构成:检测方法的特点是:制备检测FUT1基因突变的引物; 通过使用制备的引物的PCR和从猪收集的试验样品检测显示出对猪流行性腹泻的抗性的猪。 其中引物选自Int 1(SEQ ID NO:1),Int 2(SEQ ID NO:2),Ext 1(SEQ ID NO:3),Ext 2(SEQ ID NO:4)和Ext 2- 2(SEQ ID NO:5); PCR产物进行琼脂糖凝胶电泳。
    • 3. 发明授权
    • 포유동물의 세포퇴화 관련 유전자의 염기서열
    • PBLUESCRIPT-MDF的基本序列
    • KR100261869B1
    • 2000-08-01
    • KR1019970052590
    • 1997-10-14
    • 최윤재
    • 김영민이주용고윤성하석호최윤재김형기이대연
    • C12N15/12
    • PURPOSE: The base sequence of a gene related to cell degeneration of mammals is provided, which is useful in figuring out the causes of breast cancer and diagnosing the disease more correctly. CONSTITUTION: The base sequence of a gene related to cell degeneration is obtained by the following steps of: i) preparing mammary gland cells; ii) obtaining total RNA from the degenerated cells; iii) making cDNA library; iv) performing mRNA differential display PCR to identify kinds of mRNAs which are differently expressed during the degeneration of mammary gland cells; v) performing Northern blotting with PCR products to identify the patterns of gene expression; vi) performing TA-cloning with PCR fragments using pGEM-T vector and sequencing the cloned cDNA fragments; and vii) screening cDNA and analyzing base sequences.
    • 目的:提供与哺乳动物细胞变性有关的基因的碱基序列,可用于确定乳腺癌的病因和诊断疾病。 构成:通过以下步骤获得与细胞变性相关的基因的碱基序列:i)制备乳腺细胞; ii)从退化细胞获得总RNA; iii)制作cDNA文库; iv)进行mRNA差异显示PCR以鉴定在乳腺细胞退化过程中不同表达的mRNA的种类; v)用PCR产物进行Northern印迹以鉴定基因表达的模式; vi)使用pGEM-T载体用PCR片段进行TA克隆,并测序克隆的cDNA片段; 和vii)筛选cDNA并分析碱基序列。
    • 6. 发明授权
    • 우유중 CLA 함량을 높이는 사료 및 그 제조방법
    • CLA CLA CLA CLA CLA CLA CLA CLA법법법법법법법법법법법법법
    • KR100431906B1
    • 2004-05-17
    • KR1020010063661
    • 2001-10-16
    • 주식회사 카길애그리퓨리나최윤재
    • 최윤재이보균이홍구
    • A23K1/00
    • PURPOSE: A method of preparing the titled feed by adding heat treated full fat soybean and linseed oil to feed and then heat treating the obtained feed for dairy cow within a short time is provided. Thus obtained feed is suitable for normal digestive physiology of diary cows which increase the CLA content of cow's milk without reduction of milk fat and the quantity of milk. CONSTITUTION: The titled feed is prepared by mixing 25% by weight of heat treated full fat soybean with a mixture and then heat treating at 120 to 150deg.C for 0.2 to 9.4sec to produce feed. The mixture contains 7 to 16% by weight of processed corn, 6 to 12% by weight of wheat, 7 to 18% by weight of a corn by-product, 0.01 to 3% by weight of defatted bran, 6 to 9% by weight of soybean pod, 3 to 7% by weight of lupin skin, 1 to 5% by weight of wheat flour, 1 to 7% by weight of wheat bran, 17 to 23% by weight of vegetable meals, 1 to 2% by weight of limestone, 0.1 to 1.0% by weight of defluorinated phosphoric acid, 0.5 to 1.5% by weight of salt, 5 to 7% by weight of molasses, 0.1 to 0.2% by weight of mineral additives and 0.01 to 0.1% by weight of vitamin additives.
    • 目的:提供一种通过加入热处理后的全脂大豆和亚麻子油来饲喂并且然后在短时间内对获得的用于奶牛的饲料进行热处理来制备标题饲料的方法。 这样获得的饲料适合日常奶牛的正常消化生理学,其增加牛奶中的CLA含量而不减少乳脂肪和牛奶的量。 组成:标题饲料是通过将25重量%热处理过的全脂大豆与一种混合物混合,然后在120至150℃下热处理0.2至9.4秒以制备饲料来制备的。 该混合物含有7-16重量%的加工玉米,6-12重量%小麦,7-18重量%玉米副产物,0.01-3重量%脱脂麸皮,6-9重量% 大豆荚重量,3至7重量%羽扇豆皮,1至5重量%小麦粉,1至7重量%小麦麸皮,17至23重量%蔬菜粉,1至2重量% 石灰石的重量,0.1至1.0重量%的脱氟磷酸,0.5至1.5重量%的盐,5至7重量%的糖蜜,0.1至0.2重量%的矿物添加剂和0.01至0.1重量%的 维生素添加剂
    • 7. 发明授权
    • 루미노코쿠스알부스유래의베타-글루코시다제유전자
    • 源自白色瘤胃球菌的β-葡糖苷酶基因
    • KR100341451B1
    • 2002-10-30
    • KR1019980047373
    • 1998-11-05
    • 최윤재
    • 최윤재조광근우정희김성찬
    • C12N15/52
    • 본 발명은 루미노코쿠스 알부스(
      Ruminococcus albus ) 유래의 베타-글루코시다제 유전자의 염기서열을 결정하고 이를 도입하여 구축한 발현벡터로 형질전환된 형질전환체를 배양하는 유전공학 기법을 이용하여 베타-글루코시다제 효소를 대량 생산하는 방법에 관한 것으로, 루미노코쿠스 알부스의 염색체 DNA를 제한효소로 절단하여 DNA 라이브러리를 제조한 후 이 DNA 라이브러리로부터 얻은 적정 크기의 DNA 단편을 pUC19 벡터에 삽입하고 이 벡터로 대장균을 형질전환시킨 후 배양하여 베타-글루코시다제 유전자를 클로닝하고 상기 클로닝한 베타-글루코시다제 유전자를 지닌
      E.coli 형질전환체를 선별한 다음 배양하고 이 배양액의 베타-글루코시다제 효소 활성을 측정하여 베타-글루코시다제 효소 발현을 확인하므로써 루미노코쿠스 알부스의 베타-글루코시� �제 유전자가 도입되어 형질전환된 대장균 배양에 의해 베타-글루코시다제 효소를 대량 생산할 수 있는 뛰어난 효과가 있다.