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    • 1. 发明公开
    • 에스트로겐성 화합물의 검출을 위한 유전자 변이 박테리아 균주 및 이를 이용한 에스트로겐성 화합물의 검출 방법
    • 用于检测雌激素化合物的方法和使用该方法检测雌激素化合物的方法
    • KR1020170112017A
    • 2017-10-12
    • KR1020160038444
    • 2016-03-30
    • 한양대학교 산학협력단
    • 이진원계명찬유수현김영필
    • C12N15/74C07K14/195C07K14/72C12Q1/02
    • C07K14/195C07K14/72C12N15/74C12Q1/02
    • 본발명은에스트로겐성화합물의검출을위한유전자변이박테리아균주및 이를이용한에스트로겐성화합물의검출방법에관한것으로서, 더욱구체적으로는, 에스트로겐수용체의리간드결합부위 (ER LBD)와상호작용하는공동조절인자를코딩하는유전자가λCI 단백질을코딩하는유전자와접합된염기서열을포함하는플라스미드 A; 및에스트로겐수용체의리간드결합부위 (ER LBD)를코딩하는유전자가αNTD 단백질을코딩하는유전자와접합된플라스미드 B에의해서형질전환된 (transformed), 에스트로겐성화합물의검출능을갖는박테리아균주및 이를이용한에스트로겐성화합물의검출방법에관한것이다. 본발명에따르면, 인체로부터유래된에스트로겐수용체단백질을기반으로하기때문에친환경적이고, 비교적단순한공정에의해서, 낮은원가및 노동력으로, 매우짧은검출시간을갖는에스트로겐성화합물의검출을위한유전자변이박테리아및 이를이용한에스트로겐성화합물의검출방법을제공할수 있다.
    • 用于检测化合物的雌激素本发明的转基因细菌菌株和涉及使用相同的雌激素化合物的检测方法,更具体地说,编码涡共调控元件的雌激素受体的,与配体结合位点相互作用(ER LBD) A包含与编码λC蛋白的基因连接的核苷酸序列; 和雌激素通过用编码αNTD蛋白B至(变换),雌激素化合物的基因的质粒结合的受体使用具有编码基因的检测功能的相同的细菌菌株和雌激素转化为配体结合区域(ER LBD) 本发明涉及用于检测化合物的方法。 根据本发明,由于它是基于来自人友好并且相对通过简单的工艺得到的雌激素受体蛋白质,在成本和劳动力低,该基因用于检测雌激素化合物的具有非常短的检测时间变化的细菌和这 可以提供检测使用的雌激素化合物的方法。
    • 4. 发明公开
    • 탄산무수화 효소 및 포스포에놀피루브산 카르복실화 효소를 이용한 유기산 생산 시스템
    • 使用碳酸酐和磷酸羟丙酯羧酸的有机酸合成体系
    • KR1020130128663A
    • 2013-11-27
    • KR1020120052556
    • 2012-05-17
    • 한양대학교 산학협력단
    • 진언선장광석전한철백승필구만복황이택기미란민기하정진양강해민윤현식김세경노용호이진원
    • C12P7/54C12N15/70
    • C12P7/54C12N15/70C12Y402/01001
    • The present invention relates to an organic acid production system using carbonic anhydrase and phosphoenolpyruvate carboxylase. Provided are a method for producing oxaloacetic acid comprising: a bicarbonate production step for making CO2 react with H2O under the presence of the carbonic anhydrase; and an oxaloacetate production step for making the bicarbonate react with phosphoenolpyruvate under the presence of the phosphoenolpyruvate carboxylase, and a transformant, in which an expression vector comprising carbonic anhydrase (CA) and phosphoenolpyruvate carboxylase (PEPCase) is transformed to the host cell, for producing the oxaloacetic acid. The organic acid production system according to the present invention has advantages of mass-producing the organic acid with higher yield, continuously producing the organic acid, and being possibly combined with carbon dioxide reduction technology by using the carbonic anhydrase and phosphoenolpyruvate carboxylse simultaneously. [Reference numerals] (AA) Before reaction;(BB) After reaction
    • 本发明涉及使用碳酸酐酶和磷酸烯醇丙酮酸羧化酶的有机酸生产系统。 提供一种生产草酰乙酸的方法,包括:在碳酸酐酶存在下,使CO 2与H 2 O反应的碳酸氢盐生产步骤; 以及在磷酸烯醇丙酮酸羧化酶存在下使碳酸氢盐与磷酸烯醇丙酮酸反应的草酰乙酸酯生产步骤和将含有碳酸酐酶(CA)和磷酸烯醇丙酮酸羧化酶(PEPCase)的表达载体转化到宿主细胞的转化体, 草酰乙酸。 根据本发明的有机酸生产系统具有通过同时使用碳酸酐酶和磷酸烯醇丙酮酸羧基,以较高的产率批量生产有机酸,连续生产有机酸,并且可能与二氧化碳还原技术相结合的优点。 (标号)(AA)反应前;(BB)反应后
    • 7. 发明公开
    • 유해조류 제어용 살조세균
    • 有害的ALGAL物种 - 特异性杀菌细菌
    • KR1020130009902A
    • 2013-01-24
    • KR1020110069248
    • 2011-07-13
    • 한양대학교 산학협력단서울대학교산학협력단
    • 한명수이진원강윤호강수경이은선강사욱
    • C12N1/20A01N63/02C12R1/225
    • Y02A20/402
    • PURPOSE: A composition containing a certain algicidal bacteria for controlling harmful algae is provided to suppress abnormal proliferation of the harmful algae and to prevent damage due to the harmful algae and water pollution. CONSTITUTION: Lactobacillus sp. or Lactococcus sp. algicidal bacteria are used for controlling proliferation of harmful algae. The harmful algae cause water bloom. A composition for controlling the harmful algae contains the algicidal bacteria or a material derived from the algicidal bacteria. The material derived from algicidal bacteria is prepared from a petroleum ether, diethyl ether, chloroform ethyl acetate, or N-butanol fraction of the algicidal bacteria. A method for controlling harmful algae comprises a step of treating the composition to an area where the harmful algae exist.
    • 目的:为防止有害藻类的异常增殖,防止有害藻类和水污染造成的损害,提供含有一定杀藻细菌的组合物。 构成:乳杆菌属 或乳球菌属 杀藻细菌用于控制有害藻类的增殖。 有害藻类会引起水华。 用于控制有害藻类的组合物含有杀藻细菌或来自杀藻细菌的物质。 来自杀藻细菌的物质由石油醚,二乙醚,氯仿乙酸乙酯或杀藻细菌的N-丁醇级分制备。 控制有害藻类的方法包括将组合物处理到存在有害藻类的区域的步骤。