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1. 发明公开

KR1020130128663A 탄산무수화 효소 및 포스포에놀피루브산 카르복실화 효소를 이용한 유기산 생산 시스템 审中-实审
标题翻译：使用碳酸酐和磷酸羟丙酯羧酸的有机酸合成体系
公开(公告)号：KR1020130128663A
公开(公告)日：2013-11-27
申请号：KR1020120052556
申请日：2012-05-17
申请人： 한양대학교 산학협력단
发明人： 진언선 , 장광석 , 전한철 , 백승필 , 구만복 , 황이택 , 기미란 , 민기하 , 정진양 , 강해민 , 윤현식 , 김세경 , 노용호 , 이진원
IPC分类号： C12P7/54 , C12N15/70
CPC分类号： C12P7/54 , C12N15/70 , C12Y402/01001
摘要： The present invention relates to an organic acid production system using carbonic anhydrase and phosphoenolpyruvate carboxylase. Provided are a method for producing oxaloacetic acid comprising: a bicarbonate production step for making CO2 react with H2O under the presence of the carbonic anhydrase; and an oxaloacetate production step for making the bicarbonate react with phosphoenolpyruvate under the presence of the phosphoenolpyruvate carboxylase, and a transformant, in which an expression vector comprising carbonic anhydrase (CA) and phosphoenolpyruvate carboxylase (PEPCase) is transformed to the host cell, for producing the oxaloacetic acid. The organic acid production system according to the present invention has advantages of mass-producing the organic acid with higher yield, continuously producing the organic acid, and being possibly combined with carbon dioxide reduction technology by using the carbonic anhydrase and phosphoenolpyruvate carboxylse simultaneously. [Reference numerals] (AA) Before reaction;(BB) After reaction
摘要翻译：本发明涉及使用碳酸酐酶和磷酸烯醇丙酮酸羧化酶的有机酸生产系统。提供一种生产草酰乙酸的方法，包括：在碳酸酐酶存在下，使CO 2与H 2 O反应的碳酸氢盐生产步骤; 以及在磷酸烯醇丙酮酸羧化酶存在下使碳酸氢盐与磷酸烯醇丙酮酸反应的草酰乙酸酯生产步骤和将含有碳酸酐酶（CA）和磷酸烯醇丙酮酸羧化酶（PEPCase）的表达载体转化到宿主细胞的转化体，草酰乙酸。根据本发明的有机酸生产系统具有通过同时使用碳酸酐酶和磷酸烯醇丙酮酸羧基，以较高的产率批量生产有机酸，连续生产有机酸，并且可能与二氧化碳还原技术相结合的优点。（标号）（AA）反应前;（BB）反应后

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