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    • 3. 发明公开
    • 마이크로타이터 배양시스템을 이용한 이타콘산 고생산성 아스퍼질러스 테리우스 균주의 대규모 스크리닝 방법
    • 使用微型微孔板发酵系统生产高效液相色谱法的大型筛选方法
    • KR1020130052220A
    • 2013-05-22
    • KR1020110117543
    • 2011-11-11
    • 한국생산기술연구원
    • 김상용신우식이도훈조진구전계택
    • C12N1/14C12N1/38C12Q1/02C12R1/66
    • PURPOSE: A method for screening a large amount of Aspergillus terreus with excellent itaconic acid productivity using a microtiter culture system is provided to quickly select mutant strains with high productivity and to reduce a screening time. CONSTITUTION: A method for screening a strain with excellent itaconic acid productivity comprises: a step of inoculating 10-20%(v/v) of Aspergillus terreus mutant strain into a microtiter plate(MTP) containing a growth medium and culturing at 28-40 deg. C and 200-250 rpm for 2-4 days; and a step of inoculating 10-20%(v/v) of the cultured Aspergillus terreus mutant strain in the microtiter plate and culturing at 28-40 deg. C and 200-270 rpm for 3-5 days. The Aspergillus terreus is induced by treating with NTG(N-methly-N'-nitro-N-nitrosoguanidine). The growth medium contains 50-60 g/L of glucose, 3-7 g/L of a yeast extract, 1-5 g/L of NH4NO3, 1-3 g/L of MgSO, and 0.01-0.04 g/L of KH2PO4. [Reference numerals] (AA) MTP cultured strain number; (BB) Temperature(°C); (CC) Inoculated amount(%); (DD) Growth culture stirring speed(rpm); (EE) Production culture stirring speed(rpm); (FF) Culture period(days); (GG) Working volume(ml); (HH) 12 well MTP; (II) 24 well MTP
    • 目的:提供一种利用微量滴定培养系统筛选大量具有优异衣康酸生产力的土曲霉的方法,以快速选择具有高生产力的突变菌株并减少筛选时间。 构成:用于筛选具有优异衣康酸生产力的菌株的方法包括:将10-20%(v / v)的土曲霉突变菌株接种到含有生长培养基的微量滴定板(MTP)中并在28-40℃培养的步骤 度。 C和200-250rpm 2-4天; 以及在微量滴定板中接种10-20%(v / v)培养的土曲霉突变菌株并在28-40℃培养的步骤。 C和200-270rpm 3-5天。 通过用NTG(N-甲基-N'-硝基-N-亚硝基胍)处理诱导土曲霉。 生长培养基含有50-60g / L葡萄糖,3-7g / L酵母提取物,1-5g / L NH 4 NO 3,1-3g / L MgSO 4和0.01-0.04g / L 磷酸二氢钾。 (AA)MTP培养菌株数; (BB)温度(℃); (CC)接种量(%); (DD)生长培养搅拌速度(rpm); (EE)生产培养搅拌速度(rpm); (FF)文化期(天); (GG)工作体积(ml); (HH)12孔MTP; (II)24孔MTP
    • 5. 发明公开
    • 이타콘산 고생산성 변이 균주, 이의 제조 방법 및 이를 이용한 이타콘산의 생산 방법
    • 异柠檬酸高产突变菌株及其制备方法,以及使用该方法生产异柠檬酸的方法
    • KR1020120116377A
    • 2012-10-22
    • KR1020120107715
    • 2012-09-27
    • 한국생산기술연구원
    • 김상용이도훈조진구전계택신우식박철환
    • C12N1/15C12N15/52C12P7/40
    • PURPOSE: A mutant strain with high productivity of itaconic acid, a manufacturing method thereof, and a manufacturing method of itaconic acid using the same are provided to reduce catabolite repression phenomenon, thereby producing the itaconic acid at high yield from the beginning of cultivation. CONSTITUTION: A manufacturing method of mutant strain with high productivity of itaconic acid comprises the following steps: inducing a strain to a mutant; selecting a strain in which catabolite repression phenomenon by selecting 2- dioxy - glucose resistant strain; inducing the strain into mutant; and sorting strains with improved membrane permeability by selecting nystatin resistant strain. The strain is Aspergillus terreus. The strain is Aspergillus terreus 2DRNR (KACC93094P). The manufacturing method of the itaconic acid comprises the following steps: cultivating a mutant strain; and obtaining the itaconic acid from the cultivating fluid. [Reference numerals] (AA) Itaconic acid concentration(g/L); (BB) Strain
    • 目的:提供衣康酸的生产率高的突变菌株及其制造方法以及使用其的衣康酸的制造方法以降低分解代谢阻抑现象,由此从培育开始以高产率生产衣康酸。 构成:衣康酸具有高生产率的突变菌株的制造方法包括以下步骤:诱导突变株的菌株; 通过选择2-二氧 - 葡萄糖抗性菌株选择其中分解代谢物抑制现象的菌株; 将菌株诱导成突变体; 并通过选择制霉菌素抗性菌株分选提高膜渗透性的菌株。 菌株是土曲霉菌。 该菌株是土曲霉2DRNR(KACC93094P)。 衣康酸的制造方法包括以下步骤:培养突变菌株; 从培养液中得到衣康酸。 (AA)衣康酸浓度(g / L) (BB)菌株