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    • 5. 发明公开
    • 다양한 지지체에 적용 가능한, 크링글 도메인 변이체를 포함하는 단백질 칩의 제조방법
    • 适用于各种支持的蛋白质芯片,包括KRINGLE域突变体及其制备方法
    • KR1020140035631A
    • 2014-03-24
    • KR1020120102111
    • 2012-09-14
    • 한국과학기술원
    • 정기준임성갑정구민성혜정
    • C12Q1/68G01N33/53
    • G01N33/6815G01N33/5302G01N33/5304
    • The present invention relates to a method for producing a protein chip capable of using, as supports, various materials such as paper, cloth, and the like, and including a kringle domain variant, and more specifically, to a method for producing a protein chip, comprising the steps of: coating a water repellent polymer on a support through initiated chemical vapor deposition (iCVD); coating a polymer having a vinyl group on the support coated with the water repellent polymer; and immobilizing a complex of a kringle domain variant having a binding capacity specific to a target molecule and an amino acid residue including a thiol group on the support. Further, the present invention provides a protein chip produced by the method and a method for diagnosing a disease by using the protein chip. According to the present invention, the materials which are cheap and easy to carry can be used as a support for the protein chip. The kringle domain variant which has excellent solubility, thermal stability, and productivity and is cheap can be used as an antibody. Further, the binding of the thiol group and the vinyl group is applied to the production of the protein chip, thereby improving the reactivity between the kringle domain variant and the target molecule, so that a protein chip having improved portability and reactivity at low costs as compared with the existing protein chip. [Reference numerals] (AA,CC) DR5 concentration (쨉M); (BB) Signal intensity
    • 本发明涉及能够使用诸如纸,布等各种材料作为支撑体的蛋白质芯片的制造方法,并且包括三环结构域变体,更具体地,涉及一种蛋白质芯片的制造方法 包括以下步骤:通过引发的化学气相沉积(iCVD)在支撑体上涂覆斥水聚合物; 在涂覆有防水聚合物的载体上涂布具有乙烯基的聚合物; 并将具有对靶分子特异性的结合能力的三环结构域变体和包含巯基的氨基酸残基的复合物固定在载体上。 此外,本发明提供了通过使用蛋白质芯片的方法和诊断疾病的方法制造的蛋白质芯片。 根据本发明,便宜且易于携带的材料可用作蛋白质芯片的载体。 具有优异的溶解性,热稳定性和生产率并且便宜的三环结构域变体可用作抗体。 此外,将硫醇基和乙烯基的结合应用于蛋白质芯片的制备,从而提高了三环结构域变体和靶分子之间的反应性,从而以低成本改善了携带性和反应性的蛋白质芯片, 与现有蛋白质芯片相比。 (参考数字)(AA,CC)DR5浓度(쨉M); (BB)信号强度