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1. 发明公开

KR1020140053945A 평행 결합 구조의 이중 혼성화 ＰＮＡ 프로브 시스템 审中-实审
标题翻译：具有并联结构的NESTED混合PNA探针系统
公开(公告)号：KR1020140053945A
公开(公告)日：2014-05-08
申请号：KR1020147000536
申请日：2012-07-10
申请人： 주식회사 파나진
发明人： 김성기 , 박창식 , 조군호 , 박희경 , 최지아 , 최성록 , 김수남 , 김용태 , 김세련
IPC分类号： C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6841 , C12Q2525/107 , C12Q2535/131 , C12Q2561/113 , C12Q2565/1015 , C12Q2565/107
摘要： The present invention relates to two real-time nuclear acid-amplifying peptide nucleic acid (PNA) probe systems of a parallel binding structure that can make a dual hybrid with a target nucleotide, a composition including same, and a detection method using same. By means of the composition including the PNA probe system according to the present invention, the presence of a nucleotide to be detected can be confirmed and the amount thereof can be quantitatively discerned. Also, sequence variation, especially single nucleotide Polymorphism (SNP) that has been difficult to detect through existing techniques can be detected through excellent sensitivity, and at least two kinds of nucleotides existing in a sample can be simultaneously and multiply detected.
摘要翻译：本发明涉及可以与靶核苷酸形成双重杂交的并行结合结构的两个实时核酸扩增多肽核酸（PNA）探针系统，包含其的组合物及其检测方法。通过包括本发明的PNA探针系统的组合物，可以确认待检测的核苷酸的存在，并且其量可以定量地鉴定。此外，通过现有技术难以检测的序列变异，特别是单核苷酸多态性（SNP）可以通过优异的灵敏度检测，并且可以同时多次检测样品中存在的至少两种核苷酸。

2. 发明公开

KR1020140046688A 리포터 및 소광자가 결합된 PNA 프로브를 이용한 융해곡선 분석방법, 융해곡선 분석을 통한 염기다형성 분석방법 및 염기다형성 분석 키트. 审中-实审
标题翻译：使用包含报告人和QU QU QU QU QU QU ING ING ING ING IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS
公开(公告)号：KR1020140046688A
公开(公告)日：2014-04-21
申请号：KR1020120112365
申请日：2012-10-10
申请人： 주식회사 파나진
发明人： 조군호 , 김성기 , 박희경 , 박창식 , 김세련 , 김용태 , 김수남
IPC分类号： G01N27/327 , C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q1/6818 , C12Q2525/107
摘要： The present invention relates to a PNA probe to detect a nucleotide polymorphism of a target gene, a melting profile analysis method to detect a nucleotide polymorphism of a target gene using the PNA probe, a method to analyze a nucleotide polymorphism of a target gene comprising the melting profile analysis method, and a kit to detect a nucleotide polymorphism of a target gene comprising the PNA probe. According to the present invention, the PNA probe can have a negative charge. According to the present invention, a transformed PNA probe has a high recognition ability and combining ability with a target DNA by including a molecule with a negative charge, and shows two melting profile graphs by being rapidly dissociated by heat. Therefore, the analysis in a heteroconjugated sample is relatively facilitated, and at least two single base sequence variations adjacent to each other are analyzed at the same time. [Reference numerals] (AA,BB,CC,DD) PNA probe; (EE,FF) Temperature (T)
摘要翻译：本发明涉及用于检测靶基因的核苷酸多态性的PNA探针，使用该PNA探针检测靶基因的核苷酸多态性的熔解曲线分析方法，分析靶基因的核苷酸多态性的方法，熔解曲线分析方法和用于检测包含PNA探针的靶基因的核苷酸多态性的试剂盒。根据本发明，PNA探针可以具有负电荷。根据本发明，通过包含具有负电荷的分子，转化的PNA探针具有高的识别能力和与目标DNA的结合能力，并且通过热快速解离显示出两个熔解曲线图。因此，异源结合样本中的分析相对便利，并且同时分析彼此相邻的至少两个单碱基序列变异。（标号）（AA，BB，CC，DD）PNA探针; （EE，FF）温度（T）

3. 发明公开

KR1020120073536A 다중전하를 갖는 ＰＮＡ无效
标题翻译：多肽核酸具有多重功能
公开(公告)号：KR1020120073536A
公开(公告)日：2012-07-05
申请号：KR1020100135333
申请日：2010-12-27
申请人： 주식회사 파나진
发明人： 김성기 , 박창식 , 조군호 , 오수영 , 윤진원 , 주영순 , 최성록 , 김선영
IPC分类号： C07H19/00 , C07H21/00 , C07K14/00 , C12N15/11
CPC分类号： C07H19/00 , C07H21/00 , C07H21/02 , C07K14/00 , C12N15/11
摘要： PURPOSE: A PNA with multiple electric charge is provided to improve cell permeability without additional materials such as CPPs or cell penetrating reagent. CONSTITUTION: A PNA with multiple electric charge is denoted by chemical formula 1. In chemical formula 1, n is 8 or integer which is greater than 8; and C and D is independently hydrogen, hydroxyl, alkyl oxy with or without a substituent, aryl oxy with or without a substituent, amino with or without a substituent, alkyl with or without a substituent, acryl with or without a substituent, sulfonyl with or without a substituent, or aryl with or without a substituent.
摘要翻译：目的：提供具有多次电荷的PNA以提高细胞渗透性，而不需要额外的材料，如CPP或细胞穿透试剂。构成：具有多个电荷的PNA由化学式1表示。在化学式1中，n为8或大于8的整数; 并且C和D独立地是氢，羟基，具有或不具有取代基的烷基氧，具有或不具有取代基的芳氧基，具有或不具有取代基的氨基，具有或不具有取代基的烷基，具有或不具有取代基的烷基，没有取代基，或具有或不具有取代基的芳基。

4. 发明授权

KR102012690B1 평행 결합 구조의 이중 혼성화 ＰＮＡ 프로브 시스템 有权
公开(公告)号：KR102012690B1
公开(公告)日：2019-08-21
申请号：KR1020147000536
申请日：2012-07-10
申请人： 주식회사 파나진
发明人： 김성기 , 박창식 , 조군호 , 박희경 , 최지아 , 최성록 , 김수남 , 김용태 , 김세련
IPC分类号： C12N15/11 , C12Q1/68

5. 发明授权

KR102018317B1 리포터 및 소광자가 결합된 PNA 프로브를 이용한 융해곡선 분석방법, 융해곡선 분석을 통한 염기다형성 분석방법 및 염기다형성 분석 키트. 有权
公开(公告)号：KR102018317B1
公开(公告)日：2019-09-05
申请号：KR1020120112365
申请日：2012-10-10
申请人： 주식회사 파나진
发明人： 조군호 , 김성기 , 박희경 , 박창식 , 김세련 , 김용태 , 김수남
IPC分类号： G01N27/327 , C12Q1/68

6. 发明公开

KR1020130008283A 평행결합 구조의 이중 혼성화 ＰＮＡ 프로브 시스템을 포함하는 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 비결핵 항산균의 동시 검출용 조성물 및 이를 이용한 검출 방법 无效
标题翻译：用于检测MYCOBACTERIUM TUBERCULOSIS的组合物和包含平行结合结构的PNA探针系统的非结核性MYCOBACTERIA及其检测方法
公开(公告)号：KR1020130008283A
公开(公告)日：2013-01-22
申请号：KR1020110068939
申请日：2011-07-12
申请人： 주식회사 파나진
发明人： 김성기 , 박창식 , 조군호 , 박희경 , 김지현 , 최지아 , 최성록 , 김수남 , 김용태 , 김세련 , 장현정
IPC分类号： C12N15/11 , C12Q1/68 , C12Q1/04
CPC分类号： C12Q1/689 , C12Q1/6851 , C12Q2600/16 , G01N2333/35 , C12Q1/04 , C12Q2525/107 , C12Q2537/143 , C12Q2549/119 , C12Q2561/113
摘要： PURPOSE: A composition for simultaneously detecting Mycobacterium tuberculosis and non-tuberculous mycobacteria based on a PNA probe system is provided to ensure excellent sensitivity through real-time multiplex PCR and to accurately detect Mycobacterium tuberculosis and non-tuberculous mycobacteria in one tube. CONSTITUTION: A composition for simultaneously detecting Mycobacterium tuberculosis and non-tuberculous mycobacteria contains: a PNA probe which specifically binds to Mycobacterium tuberculosis(MTB) and a PNA probe which binds to the MTB-specific PNA probe in parallel; and a PNA probe which specifically binds to non-tuberculous mycobacteria(NTM) and a PNA probe which binds to the NTM-specific PNA probe. The MTP-specific PNA probe and the PNA probe which binds to the MTP-specific PNA probe in parallel have sequences of sequence numbers 10 and 14. The NTM-specific PNA probe and the PNA probe which binds to the NTM-specific PNA probe in parallel have a sequence of sequence number 11 or 12 and a sequence of sequence number 13. Each probe further contains a reporter molecule or quencher molecule. A kit for simultaneously detecting MTB and NTM contains the composition. [Reference numerals] (A1,A2) First probe; (B1,B2) Second probe; (C1,C3,C2,C4) N'(or C), C'(or N); (D1,D2) Target nucleotide; (E1,E2) Reporter(fluorescent material); (F1,F2) Quencher(quenching material); (G1,G2) Reporter or quencher
摘要翻译：目的：提供一种基于PNA探针系统同时检测结核分枝杆菌和非结核分枝杆菌的组合物，通过实时多重PCR确保优异的灵敏度，并在一个管中准确检测结核分枝杆菌和非结核分枝杆菌。构成：用于同时检测结核分枝杆菌和非结核分枝杆菌的组合物包含：特异性结合结核分枝杆菌（MTB）的PNA探针和平行结合MTB特异性PNA探针的PNA探针; 和特异性结合非结核分枝杆菌（NTM）的PNA探针和结合NTM特异性PNA探针的PNA探针。并行MTP特异性PNA探针与MTP特异性PNA探针结合的PNA探针和序列号为10和14的序列。NTM特异性PNA探针和与NTM特异性PNA探针结合的PNA探针平行具有序列号11或12的序列和序列号13的序列。每个探针还含有报道分子或猝灭剂分子。用于同时检测MTB和NTM的试剂盒含有该组合物。（参考号）（A1，A2）第一探头; （B1，B2）第二探针; （C1，C3，C2，C4）N'（或C），C'（或N）; （D1，D2）靶核苷酸; （E1，E2）记者（荧光材料）; （F1，F2）猝灭剂（淬火材料）; （G1，G2）记者或猝灭剂

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