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    • 1. 发明公开
    • 영지버섯의 균사체 액체배양으로부터 생산되는 항당뇨효과를 갖는 세포외다당체 또는 세포내다당체
    • 具有抗糖尿病和高血压作用的外分泌或多内生动物,由甘露糖脂浸出物培养生产
    • KR1020050019499A
    • 2005-03-03
    • KR1020030057304
    • 2003-08-19
    • 주식회사 티지 바이오텍양병근송치현
    • 양병근송치현
    • A61K36/074A61P3/10
    • A61K36/074A23L33/10A23V2002/00A23V2200/3262A23V2200/328A23V2250/208Y10S514/866
    • PURPOSE: Exo- or endo-biopolymers having anti-diabetic effects, produced from a submerged mycelial culture of Ganoderma lucidum are provided, which exo- or endo-biopolymers reduce concentration of glucose, total cholesterol, LDL cholesterols, triglyceride, phospholipid, alanine transaminase and aspartate transaminase in serum, arteriosclerosis index, and concentration of total cholesterol, triglyceride and phospholipid in liver, and increase insulin and HDL cholesterols in serum. CONSTITUTION: The exo- or endo-biopolymers having anti-diabetic effects are produced from the submerged mycelial culture of Ganoderma lucidum, wherein the endo-biopolymer is produced by centrifuging the submerged mycelial culture of Ganoderma lucidum, extracting the centrifuged mycelium portion with hot water, centrifuging the hot water extract and collecting the supernatant, adding ethanol into the supernatant, centrifuging the solution where ethanol is added, freeze-drying the centrifuged extract portion, dissolving the freeze-dried extract in distilled water, centrifuging the solution, dialyzing the supernatant for 3 days, and recovering the freeze-dried dialyzed solution; and the exo-biopolymer is produced by centrifuging the submerged mycelial culture of Ganoderma lucidum, adding ethanol into the supernatant, centrifuging the solution where ethanol is added, freeze-drying the centrifuged extract portion, dissolving the freeze-dried extract in distilled water, centrifuging the solution, dialyzing the supernatant for 3 days, and recovering the freeze-dried dialyzed solution. A composition for treatment of diabetes and hyperlipemia comprises the exo- or endo-biopolymers having anti-diabetic effects, produced from the submerged mycelial culture of Ganoderma lucidum.
    • 目的:提供从灵芝的浸没式菌丝体培养物产生的具有抗糖尿病作用的外源或内生物聚合物,外源或内生物聚合物降低葡萄糖,总胆固醇,低密度胆固醇,甘油三酯,磷脂,丙氨酸转氨酶 和血清中天冬氨酸转氨酶,动脉硬化指数和肝脏中总胆固醇,甘油三酯和磷脂的浓度,并增加血清中的胰岛素和HDL胆固醇。 构成:具有抗糖尿病效应的外生物或内生物聚合物由灵芝的浸没式菌丝体培养物产生,其中内生物聚合物通过将灵芝的浸没式菌丝体培养物离心制备,用热水提取离心的菌丝体部分 离心收集上清液,加入乙醇加入上清液中,离心分离出乙醇,冷冻干燥离心提取液,将冷冻干燥的萃取液溶解于蒸馏水中,离心溶液,透析上清液 3天,并回收冷冻干燥的透析溶液; 通过将灵芝的浸没式菌丝体培养物离心,向上清液中加入乙醇,离心加入乙醇的溶液,冷冻干燥离心的提取物部分,将冷冻干燥的提取物溶解在蒸馏水中,离心分离,生成外生物聚合物 溶液,将上清液透析3天,并回收冷冻干燥的透析溶液。 用于治疗糖尿病和高脂血症的组合物包含由灵芝的浸没式菌丝体培养物产生的具有抗糖尿病作用的外生物或内生物聚合物。
    • 2. 发明公开
    • 영지버섯(Ganoderma lucidum) TG(KCTC 10241BP) 균사체 배양액으로부터 고분자물질의 생산방법 및 그 용도
    • 甘草酸甘油三酯(KCTC 10241BP)的培养基中制备外聚合物的方法及其用途
    • KR1020030097062A
    • 2003-12-31
    • KR1020020034215
    • 2002-06-19
    • 송치현주식회사 티지 바이오텍
    • 송치현양병근
    • C12P1/02
    • PURPOSE: A method for preparing exo-polymers from the mycelial culture of Ganoderma lucidum TG(KCTC 10241BP) and the use thereof are provided. The exo-polymers increase glycogen concentration in muscle and the liver of a mouse, decrease lactic acid accumulation in the serum of a mouse and increase movement of a mouse. CONSTITUTION: A method for preparing exo-polymers from the mycelial culture of Ganoderma lucidum TG(KCTC 10241BP) comprises the steps of: (a) inoculating a mycelium of Ganoderma lucidum TG(KCTC 10241BP) in a synthetic medium and culturing at 25 deg. C and 100 to 150 rpm for 5 to 15 days; (b) homogenizing the cultured medium in ice water; (c) inoculating the homogenized medium to another synthetic medium and culturing it under conditions of 1vvm, pH 4 to 7, 20 to 35 deg. C and 50 to 150 rpm for 10 to 30 days; (d) centrifuging the medium at 5,000 to 15,000xg for 10 to 30 minutes and isolating the supernatant; and (e) treating the supernatant with ethanol, dissolving the precipitate in distilled water, centrifuging the solution at 5,000 to 15,000xg for 10 to 30 minutes to isolate the supernatant and dialyzing the supernatant.
    • 目的:提供灵芝菌丝体培养物(KCTC 10241BP)制备外源聚合物的方法及其应用。 外源聚合物增加肌肉和小鼠肝脏中的糖原浓度,降低小鼠血清中的乳酸积累并增加小鼠的运动。 构成:从灵芝TG(KCTC 10241BP)的菌丝体培养物制备外部聚合物的方法包括以下步骤:(a)将灵芝菌丝体(KCTC 10241BP)接种在合成培养基中并在25℃培养。 C和100至150rpm 5至15天; (b)将培养基在冰水中匀浆; (c)将均质培养基接种到另一种合成培养基中,并在1vvm,pH4至7,20至35℃的条件下培养。 C和50至150rpm 10至30天; (d)将培养基以5,000至15,000xg离心10至30分钟并分离上清液; 和(e)用乙醇处理上清液,将沉淀物溶解在蒸馏水中,以5000-15000xg离心10-30分钟,分离上清液并透析上清液。
    • 4. 发明公开
    • 균사체 액체배양에 의해 생산된 표고버섯유래세포외다당체의 당뇨병 치료용 조성물 및 그 분리방법
    • 用于治疗糖尿病的液体培养产生的含有淋巴细胞衍生的细胞多糖的糖尿病的组合物及其分离方法
    • KR1020030070440A
    • 2003-08-30
    • KR1020020009982
    • 2002-02-25
    • 신준식김동현양병근송치현주식회사 자생바이오
    • 송치현양병근김동현신준식이상철
    • C08B37/00
    • PURPOSE: A method for separating the Lentinusedodes-derived extracellular polysaccharide by the liquid culture of mycelium and a composition containing the extracellular polysaccharide for treating diabetes are provided, wherein the Lentinusedodes-derived extracellular polysaccharide shows an excellent blood sugar lowering effect. CONSTITUTION: The method comprises the steps of culturing Lentinusedodes in a potato/dextrose medium by using a rotary flask shaker under the condition of 120 rpm and 25 deg.C for 10 days; pulverizing the Lentinusedodes culture medium under the aseptic condition and injecting the pulverized one to the culture medium with a concentration of 1 %(v/v); liquid culturing the medium solution containing the pulverized one by using a rotary flask shaker under the condition of 120 rpm and 25 deg.C for 24 hours; centrifuging the liquid culture solution with 10,447 x g/20 min to obtain supernatant, precipitating the obtained supernatant in ethanol and centrifuging the precipitated one; and lyophilizing the obtained precipitate and obtaining a suspension by using distilled water.
    • 目的:提供通过菌丝体的液体培养物分离来源于细胞外多糖的细胞外多糖的方法,以及含有用于治疗糖尿病的细胞外多糖的组合物,其中来源于细胞外多糖的细胞外多糖具有优异的降血糖作用。 构成:该方法包括以120rpm和25℃条件下旋转烧瓶振荡器在马铃薯/葡萄糖培养基中培养香菇多糖的步骤10天; 在无菌条件下粉碎香菇多糖培养基,将粉碎后的培养基以1%(v / v)的浓度注入培养基中; 在120rpm,25℃的条件下,使用旋转烧瓶振荡器,液体培养含有粉碎物的培养基溶液24小时; 用10,447×g / 20分钟离心液体培养液,得到上清液,将得到的上清液沉淀在乙醇中,离心沉淀; 并将所得沉淀物冻干并通过使用蒸馏水获得悬浮液。
    • 8. 发明授权
    • 면역 증강 활성을 갖는 맛 버섯 균사체 액체배양물로부터분리한 세포내 생체고분자
    • 分离出来的嗜酸性粒细胞培养物中增加免疫刺激活性的内切葡聚糖
    • KR100673051B1
    • 2007-01-22
    • KR1020050069621
    • 2005-07-29
    • 양병근
    • 양병근송치현구영아
    • C08B37/00
    • C08B37/0033A61K36/07Y10S514/885
    • Provided is an intracellular biopolymer isolated from a submerged-liquid culture of Pholiota nameko, which is effective in increasing anti-complement, macrophages, and spleen cells and is useful for immunostimulating pharmaceutical compositions. The Pholiota nameko-derived intracellular biopolymer having an increase effect of anti-complement, macrophages, and spleen cells is separated from a submerged-liquid culture of Pholiota nameko. The intracellular biopolymer consists of a fraction comprising 79.6% of saccharide containing mannose and glucose having a molecular weight of 1000kDa as main materials and 20.4% of protein containing glycine and lysine as main materials, and a fraction comprising 86.9% of saccharide containing mannose, galactose, and glucose having a molecular weight of 3.1kDa as main materials and 13.1% of protein containing aspartic acid, threonine, glutamic acid, and glycine as main materials.
    • 提供从Pholiota nameko的浸没液体培养物分离的细胞内生物聚合物,其有效增加抗补体,巨噬细胞和脾细胞,并且可用于免疫刺激药物组合物。 从Pholiota nameko的浸没液体培养物中分离具有抗补体,巨噬细胞和脾细胞增加作用的Pholiota nameko衍生的细胞内生物聚合物。 细胞内生物聚合物由包含79.6%的含有甘露糖的糖类和分子量为1000kDa的葡萄糖作为主要材料的部分和含有甘氨酸和赖氨酸作为主要材料的蛋白质的20.4%的级分和包含86.9%的含有甘露糖,半乳糖的糖的级分组成 ,分子量为3.1kDa的葡萄糖为主要成分,含有天冬氨酸,苏氨酸,谷氨酸,甘氨酸为主要成分的蛋白质为13.1%。