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1. 发明公开

KR1020020070125A 재조합 ＨＣＶＮＳ5Ｂ 단백질, 및 그의 제조방법 및용도 无效
标题翻译：重组丙型肝炎病毒NS5B蛋白及其制备方法及其用途
公开(公告)号：KR1020020070125A
公开(公告)日：2002-09-05
申请号：KR1020020010174
申请日：2002-02-26
申请人： 주식회사 엘지생명과학
发明人： 이미경 , 김정민 , 현욱섭 , 노기윤
IPC分类号： C12N9/12
CPC分类号： C12N9/127 , C12N15/70 , C12Y207/07048 , G01N33/5767 , G01N2500/20
摘要： PURPOSE: Provided are recombinant hepatitis C virus(HCV) NS5B protein which is derived from HCV patient's serum and has excellent RNA-dependent NA polymerase(RdRp) activity, and its preparation process and use as HCV growth inhibitor. CONSTITUTION: The recombinant NS5B protein is prepared by deletion of 24 amino acids from C-terminus of HCV NS5B protein. It has RNA-dependent NA polymerase(RdRp) activity and is water soluble. It has an amino acid sequence of SEQ ID NO:8 and a nucleotide sequence of SEQ ID NO:7. An expression vector, pET21b-NS5Bt, contains a recombinant NS5B protein coding gene and transforms E.coli. The recombinant NS5B protein is prepared by culturing a transformant, E.coli BL21(DE3)/pET21b-NS5Bt(KCTC 10167BP), inducing the expression of the recombinant protein, and separating and purifying it.
摘要翻译：目的：提供源自HCV患者血清的重组丙型肝炎病毒（HCV）NS5B蛋白，具有优异的RNA依赖性NA聚合酶（RdRp）活性，其制备方法和用途为HCV生长抑制剂。构成：通过从HCV NS5B蛋白的C末端缺失24个氨基酸制备重组NS5B蛋白。它具有RNA依赖性的NA聚合酶（RdRp）活性，是水溶性的。它具有SEQ ID NO：8的氨基酸序列和SEQ ID NO：7的核苷酸序列。表达载体pET21b-NS5Bt含有重组NS5B蛋白编码基因并转化大肠杆菌。通过培养转化体大肠杆菌BL21（DE3）/ pET21b-NS5Bt（KCTC 10167BP），诱导重组蛋白的表达，分离和纯化来制备重组NS5B蛋白。

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