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    • 10. 发明公开
    • 녹용으로부터 추출된 항진균제
    • 从CERVI PARVUM CORNU分离的反真菌
    • KR1020000059468A
    • 2000-10-05
    • KR1019990007089
    • 1999-03-04
    • 전길자한소엽최원자
    • 전길자최원자한소엽이윤진박현숙김영아
    • C07F9/02A61K35/32
    • C07F9/02A61K35/32
    • PURPOSE: Provided is an antifungal composition containing the extract of cervi parvum cornu as an effective ingredient. The extract shows inhibiting activity of formation of pathogenic mycelium. CONSTITUTION: The extract of cervi parvum cornu having an antifungal effect is represented by the formula (1) and is obtained by the following steps of: extracting cervi parvum cornu with hexane; extracting the extract residues with chloroform; extracting the extract residues with ethanol; obtaining CN-E by distillation under reduced pressure, in which CN-E is further purified by solubilizing in chloroform/methanol/2.5M ammonia water(20:10:2, v/v/v) solution and isolated to obtain CN-Es, or CN-Es is further purified by solubilizing in chloroform/methanol/2.5M ammonia water(20:10:2, v/v/v) solution and isolated to obtain CN-Es and then passing through the silica gel column chromatography to obtain CN-Es-6, or CN-Es-6 is further purified by solubilizing in chloroform/methanol(2:1, v/v) solution and isolated to obtain CN-Es-6s, or CN-Es-6s is further purified by solubilizing in chloroform/methanol(2:1, 1:1, 1:1.5, v/v) solution, and chloroform/methanol/2.5M ammonia water(20:10:2, v/v/v) solution and passing through the silica gel column chromatography to obtain CN-Es-6s-F, and CN-Es-6s-F is further purified by solubilizing in chloroform/methanol(50:1, 2:1, 1:1, v/v) solution, and chloroform/methanol/2.5M ammonia water(20:10:2, v/v/v) solution and passing through the silica gel column chromatography to obtain CN-Es-6s-F-d.
    • 目的:本发明提供一种抗菌组合物,其含有小檗提取物作为有效成分。 提取物显示抑制致病菌丝体形成的活性。 构成:具有抗真菌作用的小檗提取物由式(1)表示,通过以下步骤获得:用己烷萃取小檗碱; 用氯仿萃取残留物; 用乙醇提取残留物; 通过减压蒸馏获得CN-E,其中CN-E通过在氯仿/甲醇/2.5M氨水(20:10:2,v / v / v)溶液中溶解进一步纯化,分离得到CN-E 或CN-Es通过在氯仿/甲醇/2.5M氨水(20:10:2,v / v / v)溶液中溶解进一步纯化,分离,得到CN-Es,然后通过硅胶柱色谱法 通过溶解在氯仿/甲醇(2:1,v / v)溶液中进一步纯化CN-Es-6或CN-Es-6,分离得到CN-Es-6,或CN-Es-6进一步纯化 通过溶解在氯仿/甲醇(2:1,1:1,1.5,v / v)溶液和氯仿/甲醇/2.5M氨水(20:10:2,v / v / v)溶液中的溶液和 通过硅胶柱色谱法获得CN-Es-6s-F,CN-Es-6s-F通过溶解在氯仿/甲醇(50:1,2:1,1:1,v / v)中进一步纯化 )溶液和氯仿/甲醇/2.5M氨水(20:10:2,v / v / v)溶液并通过二氧化硅 凝胶柱层析,得到CN-Es-6s-F-d。