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    • 1. 发明公开
    • 웅성 생식세포-특이 뉴클레오타이드 서열
    • 男性细胞特异性核苷酸序列
    • KR1020100025775A
    • 2010-03-10
    • KR1020080084458
    • 2008-08-28
    • 재단법인서울대학교산학협력재단
    • 한재용데반드란렝거라지
    • C12N15/12C12N5/07C12N5/02
    • PURPOSE: A kit and method for identifying microgamete are provided to detect a gene which is specifically expressed in microgamete in birds and research of identification and isolation of sperms and differentiation of birds. CONSTITUTION: A kit for identifying microgamete of animals contains a nucleotide sequence of sequence number 2, 5, 6, 10, or 12 and complementary sequence of nucleotide fragment thereof. The animals are mammals or birds. A method for identifying microgamete of animals comprises: a step of hybridizing DNA or its fragment isolated from cells with the nucleotide sequence; and a step of confirming hybridization. The animals are chicken, turkey, duck, geese, or pigeon.
    • 目的:提供一种用于鉴定微参数的试剂盒和方法,以检测在鸟类中特异性表达的基因,并研究鉴定和分离精子和分化鸟类。 构成:用于鉴定动物微参数的试剂盒含有序列号2,5,6,10或12的核苷酸序列及其核苷酸片段的互补序列。 动物是哺乳动物或鸟类。 用于鉴定动物的微参数的方法包括:将DNA或其从细胞分离的片段与所述核苷酸序列杂交的步骤; 和确认杂交的步骤。 动物是鸡,火鸡,鸭,鹅或鸽子。
    • 9. 发明公开
    • 조류 초기 배아 발달 특이적 발현 유전자
    • 在AVIANS早期胚胎发育期间差异显示的基因
    • KR1020080047652A
    • 2008-05-30
    • KR1020060117354
    • 2006-11-27
    • 재단법인서울대학교산학협력재단
    • 한재용김희발임정묵이보람
    • C12Q1/68
    • C12Q1/6888C12Q1/6844
    • Nucleotide sequences described in SEQ ID : NOs. from 1 to 40 are provided to be highly expressed in an early embryonic development stage of avians, thereby being usefully used in order to detect or identify the early embryos in stage X and HH stage 3-9. A method for identifying or detecting embryos in an early embryonic development stage of avians comprises the steps of: (a) obtaining a total RNA from the avian embryo; (b) preparing a cDNA from the total RNA; (c) hybridizing an in vitro transcripted from the avian-embryo derived cDNA or the cNDA obtained from the step(b) with at least one early embryonic-specific polynucleotide sequence selected from the group consisting of SEQ ID : NOs. from 1 to 40, a fragment sequence thereof or a complement thereof; and (d) identifying the hybridization from the step(c). A microarray or a gene amplifying kit for identifying or detecting the embryos in the early embryonic development stage of avians comprises at least one early embryonic-specific polynucleotide sequence selected from the group consisting of SEQ ID : NOs. from 1 to 40, a fragment sequence thereof or a complement thereof. Further, the avian is domestic fowl, quail, turkey, duck, goose, pheasant or dove.
    • SEQ ID NO: 提供从1到40在鸟类的早期胚胎发育阶段高度表达,从而有用地用于检测或鉴定X和HH阶段3-9期的早期胚胎。 在鸟类的早期胚胎发育阶段中鉴定或检测胚胎的方法包括以下步骤:(a)从禽胚获得总RNA; (b)从总RNA制备cDNA; (c)将从禽类衍生的cDNA或从步骤(b)得到的cNDA转录的体外杂交体与至少一种选自SEQ ID NO:1的早期胚胎特异性多核苷酸序列进行杂交。 1至40个,其片段序列或其互补序列; 和(d)鉴定来自步骤(c)的杂交。 在禽类早期胚胎发育阶段中用于鉴定或检测胚胎的微阵列或基因扩增试剂盒包含至少一种选自SEQ ID NO:1的早期胚胎特异性多核苷酸序列。 1至40个,其片段序列或其互补序列。 此外,禽鸟是家禽,鹌鹑,火鸡,鸭,鹅,雉鸡或鸽子。