专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

KR1020010036568A 외래 유전자의 정소실질을 이용한 형질전환동물 및 그 생산방법 无效
标题翻译：通过将转基因插入到测试中生产转化动物的方法
公开(公告)号：KR1020010036568A
公开(公告)日：2001-05-07
申请号：KR1019990043639
申请日：1999-10-09
申请人： 장규태 , 장희성 , 한동운 , 강문일
发明人： 장규태 , 한동운 , 장희성 , 강문일 , 김태윤 , 류재웅
IPC分类号： A01K67/027
CPC分类号： A01K67/0275 , A01K2207/12 , A01K2227/103 , A01K2227/105
摘要： PURPOSE: Provided is a method for producing the transformed animals by inserting the transgene into a testicle, thereby the insertion and expression of the transgene can be faithfully reproduced. CONSTITUTION: The method for producing the transformed animals comprises the steps of: preparing the desired transgene construct consisting of the human growth hormone(hGH) gene, the hGH receptor gene, and the rat mOB gene; preparing the liposome/DNA complex by mixing the desired transgene construct with a liposome solution; inserting the liposome/DNA complex into the testicle of a Korean male sheep; harvesting the sperm of the Korean sheep inserted by the liposome/DNA complex; fertilizing a sexual excited Korean female sheep with the harvested sperm; and producing the transformed Korean sheep.
摘要翻译：目的：提供通过将转基因插入睾丸来生产转化动物的方法，从而可以忠实地再现转基因的插入和表达。构成：生产转化动物的方法包括以下步骤：制备由人生长激素（hGH）基因，hGH受体基因和大鼠mOB基因组成的所需转基因构建体; 通过将期望的转基因构建体与脂质体溶液混合来制备脂质体/ DNA复合物; 将脂质体/ DNA复合物插入韩国雄性绵羊的睾丸中; 收获由脂质体/ DNA复合体插入的韩国羊的精子; 用收获的精子施肥性兴奋的韩国女性羊; 并生产转化的韩国羊。

2. 发明授权

KR100328511B1 mWAP/hGH이 도입된 한국재래산양 정자의 동결보존 방법 失效
标题翻译： mWAP / hGH在韩国转基因山羊中从mWAP / hGH中精液细胞的低温保存方法
公开(公告)号：KR100328511B1
公开(公告)日：2002-03-16
申请号：KR1019990043640
申请日：1999-10-09
申请人： 장규태 , 장희성 , 한동운 , 강문일
发明人： 장규태 , 한동운 , 장희성 , 강문일 , 김태윤 , 류재웅
IPC分类号： A61D19/00
摘要： 본발명은 mWAP/hGH(murine whey acidic protein/human growth hormone)이도입된한국재래산양으로부터정자를채취하여희석액으로 1차희석하고 4℃의냉장실에서 2-3시간동안냉각(0.3-0.5℃/min)을실시한다음, 상기냉동희석정액을동결보호제인글리세롤을첨가한희석액으로 2차희석하여액체질소에서보존하는방법에관한것이다. 본발명에따르면동결보존된정액의정자생존율이 60-70%로나타나, 본발명이산업적으로아주유용하게이용되어지고있는한국재래산양의대량생산은물론, 일반산양에이르기까지인공수정시인공수정에충분히이용될수 있는정자의반영구적보존이가능함으로써, 농축산업을포함한초기단계의생명발생공학에매우유용하게이용되어질수 있는발명이다.

3. 发明公开

KR1020010036569A mWAP/hGH이 도입된 한국재래산양 정자의 동결보존 방법 失效
标题翻译：来自韩国的收集的冻干干燥方法分析MWAP / HGH的基因
公开(公告)号：KR1020010036569A
公开(公告)日：2001-05-07
申请号：KR1019990043640
申请日：1999-10-09
申请人： 장규태 , 장희성 , 한동운 , 강문일
发明人： 장규태 , 한동운 , 장희성 , 강문일 , 김태윤 , 류재웅
IPC分类号： A61D19/00
CPC分类号： A61D19/021
摘要： PURPOSE: A freeze-drying method of sperm collected from Korean sheep harboring the gene of mWAP/hGH is provided, thereby the sperm can be safely stored. CONSTITUTION: The freeze-drying method of sperm collected from Korean sheep comprises the steps of: collecting sperm from Korean sheep harboring the gene of mWAP/hGH; diluting the sperm into a diluting solution(Citrate-Egg york-Tris Extender) in a volume ratio of 1:2 and cooling at 4 deg. C for 2 to 3 hours; secondly diluting the sperm solution into a diluting solution(Citrate-Egg york-Tris Extender) which contains glycerol at 3 to 5 deg. C; and inserting the sperm solution into a vessel, sealing and dipping the vessel into liquid nitrogen having -79 or -196 deg. C, in which the diluting solution contains sodium citrate, sodium phosphate diabasic, glycerin, glucose, fructose, Tris, EDTA, sodium bicarbonate, BSA, Streptomycin-penicillin-G, and Egg york.
摘要翻译：目的：提供从含有mWAP / hGH基因的韩国羊收集的精液的冷冻干燥方法，从而可以安全地储存精子。构成：从韩国羊收集的精液的冷冻干燥方法包括以下步骤：从含有mWAP / hGH基因的韩国羊收集精子; 将精子以1:2的体积比和4℃的冷却稀释到稀释溶液（柠檬酸盐 - 蛋黄 - Tris增量剂）中。 C 2〜3小时; 其次将精子溶液稀释成含有3至5度甘油的稀释溶液（柠檬酸盐 - 蛋黄 - Tris延伸剂）。 C; 并将精子溶液插入容器中，将容器密封并浸入具有-79或-196度的液氮中。 C，其中稀释溶液含有柠檬酸钠，磷酸二钠，甘油，葡萄糖，果糖，Tris，EDTA，碳酸氢钠，BSA，链霉素 - 青霉素-G和蛋黄。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式