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    • 1. 发明公开
    • 외래 유전자의 정소실질을 이용한 형질전환동물 및 그 생산방법
    • 通过将转基因插入到测试中生产转化动物的方法
    • KR1020010036568A
    • 2001-05-07
    • KR1019990043639
    • 1999-10-09
    • 장규태장희성한동운강문일
    • 장규태한동운장희성강문일김태윤류재웅
    • A01K67/027
    • A01K67/0275A01K2207/12A01K2227/103A01K2227/105
    • PURPOSE: Provided is a method for producing the transformed animals by inserting the transgene into a testicle, thereby the insertion and expression of the transgene can be faithfully reproduced. CONSTITUTION: The method for producing the transformed animals comprises the steps of: preparing the desired transgene construct consisting of the human growth hormone(hGH) gene, the hGH receptor gene, and the rat mOB gene; preparing the liposome/DNA complex by mixing the desired transgene construct with a liposome solution; inserting the liposome/DNA complex into the testicle of a Korean male sheep; harvesting the sperm of the Korean sheep inserted by the liposome/DNA complex; fertilizing a sexual excited Korean female sheep with the harvested sperm; and producing the transformed Korean sheep.
    • 目的:提供通过将转基因插入睾丸来生产转化动物的方法,从而可以忠实地再现转基因的插入和表达。 构成:生产转化动物的方法包括以下步骤:制备由人生长激素(hGH)基因,hGH受体基因和大鼠mOB基因组成的所需转基因构建体; 通过将期望的转基因构建体与脂质体溶液混合来制备脂质体/ DNA复合物; 将脂质体/ DNA复合物插入韩国雄性绵羊的睾丸中; 收获由脂质体/ DNA复合体插入的韩国羊的精子; 用收获的精子施肥性兴奋的韩国女性羊; 并生产转化的韩国羊。
    • 3. 发明公开
    • mWAP/hGH이 도입된 한국재래산양 정자의 동결보존 방법
    • 来自韩国的收集的冻干干燥方法分析MWAP / HGH的基因
    • KR1020010036569A
    • 2001-05-07
    • KR1019990043640
    • 1999-10-09
    • 장규태장희성한동운강문일
    • 장규태한동운장희성강문일김태윤류재웅
    • A61D19/00
    • A61D19/021
    • PURPOSE: A freeze-drying method of sperm collected from Korean sheep harboring the gene of mWAP/hGH is provided, thereby the sperm can be safely stored. CONSTITUTION: The freeze-drying method of sperm collected from Korean sheep comprises the steps of: collecting sperm from Korean sheep harboring the gene of mWAP/hGH; diluting the sperm into a diluting solution(Citrate-Egg york-Tris Extender) in a volume ratio of 1:2 and cooling at 4 deg. C for 2 to 3 hours; secondly diluting the sperm solution into a diluting solution(Citrate-Egg york-Tris Extender) which contains glycerol at 3 to 5 deg. C; and inserting the sperm solution into a vessel, sealing and dipping the vessel into liquid nitrogen having -79 or -196 deg. C, in which the diluting solution contains sodium citrate, sodium phosphate diabasic, glycerin, glucose, fructose, Tris, EDTA, sodium bicarbonate, BSA, Streptomycin-penicillin-G, and Egg york.
    • 目的:提供从含有mWAP / hGH基因的韩国羊收集的精液的冷冻干燥方法,从而可以安全地储存精子。 构成:从韩国羊收集的精液的冷冻干燥方法包括以下步骤:从含有mWAP / hGH基因的韩国羊收集精子; 将精子以1:2的体积比和4℃的冷却稀释到稀释溶液(柠檬酸盐 - 蛋黄 - Tris增量剂)中。 C 2〜3小时; 其次将精子溶液稀释成含有3至5度甘油的稀释溶液(柠檬酸盐 - 蛋黄 - Tris延伸剂)。 C; 并将精子溶液插入容器中,将容器密封并浸入具有-79或-196度的液氮中。 C,其中稀释溶液含有柠檬酸钠,磷酸二钠,甘油,葡萄糖,果糖,Tris,EDTA,碳酸氢钠,BSA,链霉素 - 青霉素-G和蛋黄。