专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

KR1020080042747A 신규한 융합 단백질, 이를 발현하는 세포주 및 이의 제조방법 无效
标题翻译：新型融合蛋白，表达其的细胞系及其制备方法
公开(公告)号：KR1020080042747A
公开(公告)日：2008-05-15
申请号：KR1020070114360
申请日：2007-11-09
申请人： 보령제약 주식회사
发明人： 김상린 , 단현광 , 임상민 , 구재경 , 신주엽 , 김명환 , 임종진 , 정찬희
IPC分类号： C07K19/00
CPC分类号： C07K14/505 , A61K38/1816 , A61K47/64 , C07K14/76 , C07K2319/00 , C07K2319/31 , C12N9/003 , C12N15/85 , C12N2510/02 , C12Y105/01003
摘要： A novel fusion protein of human albumin and human erythropoietin(EPO) is provided to enhance in vivo half life and blood corpuscle production-promoting ability of EPO and minimize influence to characteristics and protein three-dimensional structure of EPO by using a linker having a repetitive amino acid sequence in fusion process. A novel fusion protein is prepared by linking human albumin to human erythropoietin(EPO) by using a linker having the amino acid sequence of (GGSGG)n in which n is an integer from 3 to 5, and has the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4 or SEQ ID NO:6. A gene encoding the fusion protein has the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3 or SEQ ID NO:5. A cell line expressing the fusion protein is produced by transforming a host cell such as CHO(Chinese hamster ovary) cell with an expression vector containing a human albumin gene, a linker having the nucleotide sequence of (GGAGGAAGCGGAGGA)n and a human erythropoietin gene and an expression vector containing a gene encoding dihydrofolate reductase(DHFR). Further, the cell line is a CHO/dhfr- of which a dhfr gene is in deficiency.
摘要翻译：提供人白蛋白和人促红细胞生成素（EPO）的新型融合蛋白，以增强EPO的体内半衰期和血小板产生促进能力，并通过使用具有重复性的接头对EPO的特征和蛋白质三维结构的影响最小化融合过程中的氨基酸序列。通过使用具有（GGSGG）n的氨基酸序列的接头，其中n为3至5的整数，并且具有SEQ ID NO：1的氨基酸序列，将人白蛋白与人促红细胞生成素（EPO）连接，制备新的融合蛋白 NO：2，SEQ ID NO：4或SEQ ID NO：6。编码融合蛋白的基因具有SEQ ID NO：1，SEQ ID NO：3或SEQ ID NO：5的核苷酸序列。通过用含有人白蛋白基因的表达载体，具有（GGAGGAAGCGGAGGA）n的核苷酸序列的接头和人促红细胞生成素基因转化宿主细胞如CHO（中国仓鼠卵巢）细胞，产生表达融合蛋白的细胞系，含有编码二氢叶酸还原酶（DHFR）的基因的表达载体。此外，细胞系是CHO / dhfr-其中dhfr基因是缺乏的。

IPRDB

热门服务

关于我们

友情链接

联系方式