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    • 1. 发明授权
    • 인슐린 분비 세포주 및 이를 포함하는 이식물
    • 인슐린분비세포주및이를포함하는이식물
    • KR100463366B1
    • 2004-12-29
    • KR1020047001293
    • 1994-09-27
    • 메르크 파텐트 게엠베하
    • 체르니코우폴아스파리마리앙
    • C12N5/10C12N5/16C07K14/62
    • C12N5/0676A61K2035/126C12N5/0677C12N2510/02
    • PCT No. PCT/FR94/01129 Sec. 371 Date Jun. 4, 1996 Sec. 102(e) Date Jun. 4, 1996 PCT Filed Sep. 27, 1994 PCT Pub. No. WO95/09231 PCT Pub. Date Apr. 6, 1995The invention relates to the field of biology and, in particular, to the field of cellular biology. The invention concerns a novel glucose-sensitive cell line designated beta cell line (INS-I) expressing glucokinase and the glucose carrier Glut 2 at levels comparable with those of normal beta cells but which is, in addition, incapable of IGF-II expression-dependent proliferation because of genetic manipulation. The invention also concerns a method for the production of said novel cell line, its aggregation in the form of a pseudoislet, its immobilization in a biocompatible hydrogel and its hardening by means of a hardening solution. Application in insulin-secreting beta -cell transplants.
    • PCT No.PCT / FR94 / 01129 Sec。 371日期1996年6月4日Sec。 102(e)日期1996年6月4日PCT提交日期1994年9月27日PCT Pub。 WO95 / 09231 PCT Pub。 日期1995年4月6日本发明涉及生物学领域,特别涉及细胞生物学领域。 本发明涉及一种新的葡萄糖敏感性细胞系,其表达葡萄糖激酶和葡萄糖载体Glut2的β细胞系(INS-1)的水平与正常β细胞的水平相当,并且另外不能表达IGF- 由于遗传操作而导致的依赖增殖 本发明还涉及生产所述新细胞系的方法,其以假小室形式聚集,其固定在生物相容性水凝胶中并通过硬化溶液硬化。 应用于胰岛素分泌型β细胞移植。