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    • 4. 发明公开
    • 전자기장을 이용하여 성체줄기세포를 슈반세포로 분화시키는 방법
    • 使用电磁场将成像干细胞分化成SCHWANN细胞的方法
    • KR1020130099633A
    • 2013-09-06
    • KR1020120021285
    • 2012-02-29
    • 동국대학교 산학협력단서울대학교산학협력단
    • 서영권박정극한미정이종호윤희훈김수찬김성민
    • C12N5/074C12N13/00C12N5/079C12N5/02
    • PURPOSE: A method for differentiating adult stem cells into schwann cells is provided to effectively differentiate adult stem cells into schwann cells without an expensive differentiation medium. CONSTITUTION: A method for differentiating adult stem cells into schwann cells using an electromagnetic field comprises the steps positioning the adult stem cells in the electromagnetic field at a frequency of 1-200 Hz; and culturing the adult stem cells. The intensity of the electromagnetic field is 0.5-2 mT. The culturing comprises the steps of: culturing the adult stem cells in an alpha-MEM medium containing beta-mercaptoethanol for 12-36 hours; culturing the adult stem cells in an alpha-MEM medium containing trans-retinoic acid for 60-82 hours; and culturing the adult stem cells in an alpha-MEM medium containing forskolin for 7-14 days.
    • 目的:提供一种将成体干细胞分化为雪旺氏细胞的方法,以有效地将成体干细胞分化成施旺细胞,而不需要昂贵的分化培养基。 构成:使用电磁场将成体干细胞分化成雪旺氏细胞的方法包括以1-200Hz的频率将成体干细胞定位在电磁场中的步骤; 并培养成体干细胞。 电磁场强度为0.5-2 mT。 培养包括以下步骤:在含有β-巯基乙醇的α-MEM培养基中培养12-36小时的成体干细胞; 在含有反式视黄酸的α-MEM培养基中培养成体干细胞60-82小时; 并在含有毛喉素的α-MEM培养基中培养成体干细胞7-14天。
    • 5. 发明授权
    • 미세유체 기반의 조직 배양시 저산소 조건의 구현을 위한 세포 내 용존산소농도 제어장치 및 이를 이용한 세포 내 용존산소농도 제어방법
    • 氧化浓度控制装置及用于产生基于微流体组织培养的超临界状态的方法
    • KR101294521B1
    • 2013-08-07
    • KR1020120015365
    • 2012-02-15
    • 서울대학교산학협력단동국대학교 산학협력단
    • 박재우오수정전누리박정극
    • C12M3/00C12M1/36C12M1/04
    • C12M41/48C12M23/58C12M41/34
    • PURPOSE: An apparatus for controlling the concentration of dissolved oxygen in cells and a method for controlling the concentration of dissolved oxygen in cells using the same are provided to simplify and miniaturize the apparatus because additional devices for injecting mixed gas are unnecessary. CONSTITUTION: An apparatus for controlling the concentration of dissolved oxygen in cells comprises: a first channel (110) which provides a space for cell culture; a second channel (120) which is placed at one side of the first channel; an intermediate membrane which is placed between the first and second channels and divides for enabling oxygen to pass through the first and second channels; and an oxygen absorbent supply unit which supplies an oxygen absorbent to the second channel for discharging intracellular dissolved oxygen of the first channel to the second channel through the intermediate membrane. A method for controlling the dissolved oxygen in cells using the apparatus comprises the steps of: culturing the cells in the first channel; and supplying the oxygen absorbent to the second channel and reducing oxygen partial pressure of the first channel.
    • 目的:提供一种用于控制细胞中溶解氧浓度的装置以及使用该装置控制细胞中溶解氧浓度的方法,以便简化和小型化装置,因为不需要额外的注入混合气体的装置。 构成:用于控制细胞中溶解氧浓度的装置包括:提供细胞培养空间的第一通道(110) 第二通道(120),其设置在所述第一通道的一侧; 中间膜,其被放置在第一通道和第二通道之间,并分开以使氧能够通过第一和第二通道; 以及氧吸收剂供给单元,其向第二通道供给氧吸收剂,用于通过中间膜将第一通道的细胞内溶解氧排出到第二通道。 使用该装置控制细胞中的溶解氧的方法包括以下步骤:在第一通道中培养细胞; 以及将氧吸收剂供应到第二通道并降低第一通道的氧分压。