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    • 4. 发明公开
    • 콩 베타-콘글리시닌의 3 서브유닛들을 포함한 콩 품종 선별용 프라이머 및 이를 이용한 콩 품종 선별 방법
    • 用于选择含有β-CONGLYCININ的3个亚类的豆类变体的选择方法及其选择方法
    • KR1020130075985A
    • 2013-07-08
    • KR1020110144350
    • 2011-12-28
    • 대한민국(농촌진흥청장)
    • 양기웅김현태고종민이영훈전명기윤홍태이병원심하식정찬식백인열
    • C12N15/11C12Q1/68
    • C12Q1/6895C12Q2600/158
    • PURPOSE: Molecular markers and a detecting method using the same are provided to identify sequences of three subunits of bean beta-conglycinin by using a molecular marker, and to easily detect existence of three subunits of beta-conglycinin by directly extracting DNA from plant without conducting SDS-PAGE. CONSTITUTION: Primer pairs for selecting bean cultivar which detects a subunit of bean beta-conglycinin is selected from a group consisting of; a primer pair represented by sequence number 1 and 2; a primer pair represented by sequence number 3 and 4; a primer pair represented by sequence number 5 and 6; a primer pair represented by sequence number 7 and 8; a primer pair represented by sequence number 9 and 10; a primer pair represented by sequence number 11 and 12; and a primer pair represented by sequence number 13 and 14. A method of selecting bean cultivar which detects a subunit of bean beta-conglycinin comprises; (i) a step which separates DNA from a bean sample; (ii) a step which amplifies a target sequence by performing an amplification reaction with at least one primer pairs among the primer pairs and the separated DNA as a template; and (iii) a step which detects the amplified products.
    • 目的:提供分子标记和使用其的检测方法,通过使用分子标记鉴定豆β-伴大豆球蛋白的三个亚基的序列,并通过直接从植物中提取DNA,轻松检测β-伴大豆球蛋白的三个亚基的存在,而不进行 SDS-PAGE。 构成:选择检测豆β-伴大豆球蛋白亚基的豆类品种的引物对选自: 由序列号1和2表示的引物对; 由序列号3和4表示的引物对; 由序列号5和6表示的引物对; 由序列号7和8表示的引物对; 由序列号9和10表示的引物对; 由序列号11和12表示的引物对; 和由序列号13和14表示的引物对。选择检测豆β-伴大豆球蛋白亚基的豆品种的方法包括: (i)从豆样品中分离DNA的步骤; (ii)通过在引物对和分离的DNA之间的至少一个引物对作为模板进行扩增反应来扩增靶序列的步骤; 和(iii)检测扩增产物的步骤。
    • 6. 发明公开
    • 파종장치
    • 软件设备
    • KR1020000072237A
    • 2000-12-05
    • KR1020000048330
    • 2000-08-21
    • 대한민국(농촌진흥청장)
    • 임시규서득룡고종민홍순표곽용호
    • A01C7/06
    • PURPOSE: A sowing apparatus is provided to sow seeds and fertilizer by a proper interval uniformly by uniformly distributing blowing force of a blowing element uniformly with a plurality of ventilation holes formed on induction plates via discharging holes of conveying elements and prevent safety accidents by firmly coupling a tank and the blowing element on a top surface of a combine. CONSTITUTION: A sowing apparatus includes a tank(10) mounted on a combine(100) for receiving seeds and fertilizer, a blowing element(20) for sucking and discharging the seeds and the fertilizer received in the tank, a fixing element having a vertical supporting die(210), a lower supporting die(220) and a fixing die in the middle of the vertical supporting die for fixing the tank and the blowing element on a top part of the combine , a conveying element(30) formed with discharging holes for conveying the seeds and the fertilizer discharged by the blowing element to positions in the vicinity of the ground, a supporting element for fixing a rear part of the conveying element to a rear lower part of the combine, and an induction unit(40) for uniformly distributing the seeds and the fertilizer to the ground, wherein the induction unit includes a plurality of induction plates inserted in the conveying element to be able to control its height behind the discharging holes of the conveying element and formed with a plurality of ventilation holes.
    • 目的:提供一种播种装置,通过均匀地分配吹塑元件的吹塑力,均匀地分布有种子和肥料,均匀地分布在感应板上形成的多个通风孔,通过输送元件的排放孔,并通过牢固的连接来防止安全事故 一个坦克和组合的顶面上的吹风元件。 构成:播种装置包括安装在用于接收种子和肥料的组合体(100)上的罐(10),用于吸收和排出种子的发泡元件(20)和容纳在罐中的肥料,具有垂直 支撑模具(210),下支撑模具(220)和在垂直支撑模具的中间的固定模具,用于将油箱和吹风元件固定在组合件的顶部;输送元件(30) 用于将种子输送的孔和由吹风元件排出的肥料到地面附近的位置,用于将输送元件的后部固定到组合体的后下部的支撑元件,以及感应单元(40) 用于将种子和肥料均匀分配到地面,其中感应单元包括插入输送元件中的多个感应板,以便能够控制其在输送元件的排出孔后面的高度 并形成有多个通风孔。