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    • 2. 发明公开
    • 동물의 면역능을 증강시키는 더덕 추출물, 그 제조방법 및용도
    • 提取动物免疫功能强度的方法和用途
    • KR1020090028213A
    • 2009-03-18
    • KR1020070093644
    • 2007-09-14
    • 대한민국(농림축산식품부 농림축산검역본부장)
    • 김종만김재명이희수장금찬박신영조윤상이명헌윤소미박중원지순옥정석찬
    • A61K36/344A61P37/02
    • A manufacturing method of extract of codonopsis lanceolata is provided to increase immunomodulation enhancement of pathogenic bacteria, to be useful as immunostimulator preventing and treating efficiently disease of the animal, an adjuvant improving effect of vaccine and an adjuvant treatment making therapeutic efficacy reinforced. A manufacturing method of extract of codonopsis lanceolata comprises steps of: obtaining the first extracts and residue by heating powder of leaf and stem of the dried Condonopsis lanceolata Trautv into distilled water, separating the first extracts separately, putting it into residue, heating it obtaining the second extraction extracts; mixing the first extracts of the obtained Condonopsis lanceolata Trautv stem and leaf and the second extracts of Condonopsis lanceolata Trautv stem and leaf, putting ethanol into the mixing extract, obtaining precipitated polysaccharide by stationing the obtained solution and centrifuging the precipitated polysaccharide; and center-rinsing the separated polysaccharide to the ethanol, drying the washed material, dissolving dried material with distilled water, filtering the obtained solution and obtaining undiluted solution.
    • 提供密码子提取物的制造方法,以增加病原菌的免疫调节增强作用,有效地用于预防和治疗动物有效疾病的免疫刺激剂,疫苗的辅助改善效果和辅助治疗,增强治疗功效。 密码子提取物的制造方法包括以下步骤:通过将干燥的马铃薯干燥杆菌的叶和茎的粉末加热到蒸馏水中获得第一提取物和残留物,分离第一提取物,将其放入残渣中,加热得到 第二提取提取物; 混合所得到的Condonopsis lanceolata Trautv茎和叶的第一提取物和Condonopsis lanceolata Trautv茎和叶的第二提取物,将乙醇加入到混合提取物中,通过沉淀所得溶液并离心沉淀的多糖得到沉淀的多糖; 将分离的多糖中心漂洗至乙醇,干燥洗涤过的物质,用蒸馏水溶解干燥物,过滤得到的溶液,得到未稀释溶液。
    • 4. 发明公开
    • 캔디다 사케 용균 추출물을 포함하는 면역 증강제
    • 免疫球蛋白包括坎地迪沙星的提取物
    • KR1020090013600A
    • 2009-02-05
    • KR1020070077855
    • 2007-08-02
    • 대한민국(농림축산식품부 농림축산검역본부장)
    • 김종만김재명이희수장금찬박신영조윤상이명헌윤소미박중원지순옥정석찬
    • A61K36/06A23K1/16
    • A61K36/06
    • An immunostimulant containing the lystic extract of Candida sake is provided to increase activity of neutrophil and lymphocyte as major immune cells of animals, thereby protecting animals from diseases. The immunostimulant contains the lystic extract of Candida sake which is prepared by cultivating Candida sake in yeast malt extract agar broth at 30 deg. C for 72 hours, centrifuging the cultured fluid at 3000Xg for 30 minutes and collecting it, rinsing the collected pellet with phosphate buffered saline(PBS) of pH 7.2 and suspending the rinsed pellet in PBS, adding 5% of TNT solution containing 50mM of Tris-HCl, 0.15M of NaCl and 4% Triton x-114 as solubilizing agent into the Candida sake suspension, stirring the mixture at room temperature for 2-3 days to solubilize the Streptomyces albus, and regulating pH of the solution to 6.5-7.2, and drying the solution in the thermostatic chamber.
    • 提供含有念珠菌精液提取物的免疫刺激剂,以增加嗜中性粒细胞和淋巴细胞作为动物主要免疫细胞的活性,从而保护动物免受疾病。 免疫刺激剂含有通过在30℃下在酵母麦芽提取物琼脂培养液中培育念珠菌精油而制备的念珠菌清酒。 培养72小时,将培养液以3000Xg离心30分钟并收集,用pH7.2的磷酸盐缓冲盐水(PBS)冲洗收集的沉淀物,并将漂洗的沉淀悬浮于PBS中,加入5%含有50mM Tris -HCl,0.15M NaCl和4%Triton x-114作为增溶剂加入到念珠菌悬浮液中,在室温下搅拌混合物2-3天以溶解链霉菌,并将溶液的pH调节至6.5-7.2 ,并干燥恒温室中的溶液。