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    • 3. 发明公开
    • 단백질 합성 키트, 자동추출장비를 이용한 단백질 발현 및 추출 방법
    • 蛋白质合成试剂盒,使用自动纯化装置的蛋白质表达和纯化方法
    • KR1020130023091A
    • 2013-03-07
    • KR1020120090149
    • 2012-08-17
    • (주)바이오니아
    • 박한오조유상정준호한지원김남일
    • C07K1/32C07K1/14C12N15/09C12M1/18
    • C12P21/00B01J19/0046B01J2219/00315B01J2219/00364B01J2219/00459B01J2219/00495C07K1/22C12P21/02C07K1/32C07K1/14C12M1/18C12N15/09
    • PURPOSE: A method for expressing and isolating proteins using a protein synthesis kit and automatic purifying equipment is provided to quickly and simply obtain a plurality of proteins. CONSTITUTION: A method for expressing and isolating proteins using a protein synthesis kit and automatic purifying equipment comprises: a step of preparing a template for synthesizing cell-free proteins; a step of adding the template into a reaction solution for expressing the cell-free proteins, and expressing the cell-free proteins; a step of adding a magnetic particle which binds to an affinity tag, and adhering a target protein on the magnetic particle; and a step of isolating the adhered target protein from the magnetic particle. The automatic purifying equipment comprises: a pipette block, a fixed body, a pipette block vertical moving device, a base plate with a multiwall plate kits(420,420'), a magnetic field applying unit, and a heating unit.
    • 目的:提供使用蛋白质合成试剂盒和自动纯化设备来表达和分离蛋白质的方法以快速简单地获得多种蛋白质。 构成:使用蛋白质合成试剂盒和自动纯化装置表达和分离蛋白质的方法包括:制备用于合成无细胞蛋白质的模板的步骤; 将模板添加到用于表达无细胞蛋白质的反应溶液中并表达无细胞蛋白质的步骤; 添加与亲和标签结合的磁性粒子,将靶蛋白粘附在磁性粒子上的工序; 以及从磁性颗粒分离粘附的靶蛋白的步骤。 自动净化设备包括:移液管块,固定体,移液管块垂直移动装置,具有多壁板组件的底板(420,420'),磁场施加单元和加热单元。