专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

KR1020020082531A 강한 구조 유전자 발현을 위한 발현 카세트와 플라스미드 및 그의 사용방법 失效
标题翻译：表达强度基因表达的表达和质粒及其用途
公开(公告)号：KR1020020082531A
公开(公告)日：2002-10-31
申请号：KR1020010021962
申请日：2001-04-24
申请人： 금호석유화학 주식회사
发明人： 정화지 , 김용식 , 조정우 , 정창호
IPC分类号： C12N15/11
CPC分类号： C07K14/415 , C12N15/70 , C12N15/743 , C12N15/8225 , C12N15/8227
摘要： PURPOSE: Provided are an expression cassette and a plasmid which have a strong structural promoter capable of binding to a heterologous gene for strong constitutive gene expression and their use. CONSTITUTION: A chimeric gene is characterized by containing a promoter and heterologous nucleic acid coding a desirable polypeptide, wherein the promoter is Arabidopsis trehalose-6-phosphate synthease 3 promoter of 1kb. In particular, the promoter is characterized by: (i) being isolated from Arabidopsis thaliana; having restriction enzyme sites for SacI(0.12kb), BspHI(0.17kb), SauI(0.78kb) and SalI(1.0kb); and containing a nucleotide sequence of SEQ ID NO:1.
摘要翻译：目的：提供具有强结构启动子的表达盒和质粒，其能够结合异源基因用于强组成型基因表达及其用途。构成：嵌合基因的特征在于含有编码所需多肽的启动子和异源核酸，其中启动子是1kb的拟南芥海藻糖-6-磷酸合成酶3启动子。特别地，启动子的特征在于：（i）从拟南芥中分离; 具有SacI（0.12kb），BspHI（0.17kb），SauI（0.78kb）和SalI（1.0kb）的限制酶位点; 并含有SEQ ID NO：1的核苷酸序列。

2. 发明公开

KR1019990055910A 아마란터스 비리디스로 부터 아마란딘1과 아마란딘2의 항바이러스성 단백질 및 이를 코딩하는 디엔에이 失效
标题翻译：从amaranthus viridis，amaranthine 1和amaranthine 2和二烯的抗病毒蛋白
公开(公告)号：KR1019990055910A
公开(公告)日：1999-07-15
申请号：KR1019970075883
申请日：1997-12-29
申请人： 금호석유화학 주식회사
发明人： 김용식 , 박선충 , 오수경 , 이호설 , 조정우 , 정창호
IPC分类号： C12N15/29
摘要： 본 발명은 아마란터스 비리디스(
Amaranthus viridis )의 항바이러스성이 있는 진핵(eukaryotic) 리보솜-불활성 단백질(RIP), 아마란딘 1 과 아마란딘 2를 코딩하는 cDNA 클론과 하이브리드 벡터(hybrid vector)와 형질전환된 숙주 및 cDNA를 포함하는 각각의 물질에 관한 것이다. 더욱 상세히는 아마란딘 1 과 아마란딘 2의 전체 또는 그의 단편을 코딩하는 cDNA 염기 서열 및 이 염기서열로 형질전환된 미생물 또는 식물체를 이용하여 아마란딘 1 과 아마란딘 2, 또는 그의 단편을 제조하는 방법을 제공하는 것이다.

3. 发明公开

KR1019990055909A 광범위 바이러스 감염억제 활성을 지닌 단백질과 이의 유전자 및 균주 无效
标题翻译：具有广泛病毒感染抑制活性的蛋白质，它们的基因和菌株
公开(公告)号：KR1019990055909A
公开(公告)日：1999-07-15
申请号：KR1019970075882
申请日：1997-12-29
申请人： 금호석유화학 주식회사
发明人： 김용식 , 박선충 , 오수경 , 조정우 , 정창호
IPC分类号： C07K14/435
摘要： 본 발명은 아마란터스 비리디스(
Amaranthus viridis )의 항바이러스활성을 지닌 진핵(eukaryotic) 리보솜-불활성 단백질(RIP)인 아마란딘-S를 암호하는 cDNA 클론과 하이브리드 벡터(hybrid vector)와 형질전환된 숙주 및 cDNA를 포함하는 각각의 물질에 관한 것이다. 더욱 상세히는 아마란딘-S 전체 또는 그의 단편을 코딩하는 cDNA 염기 서열 및 이 염기서열로 형질전환된 미생물 또는 식물체을 이용하여 아마란딘-S 또는 그의 단편을 제조하는 방법을 제공하는 것이다.

4. 发明授权

KR100266229B1 아마란터스 비리디스로 부터 아마란딘1과 아마란딘2의 항바이러스성 단백질 및 이를 코딩하는 디엔에이 失效
公开(公告)号：KR100266229B1
公开(公告)日：2004-05-20
申请号：KR1019970075883
申请日：1997-12-29
申请人： 금호석유화학 주식회사
发明人： 김용식 , 박선충 , 오수경 , 이호설 , 조정우 , 정창호
IPC分类号： C12N15/29
摘要： PURPOSE: Antiviral proteins Amarandin 1 and Amarandin 2 derived from Amaranthus viridis and cDNAs encoding the same proteins are provided, thereby isolating and sequencing the antiviral proteins Amarandin 1 and Amarandin 2, and producing a large quantity of antiviral proteins Amarandin 1 and Amarandin 2 using transformation technique. CONSTITUTION: The cDNA encoding the antiviral protein Amarandin 1 has the nucleotide sequence set forth in SEQ ID NO:1. The cDNA encoding the antiviral protein Amarandin 2 has the nucleotide sequence set forth in SEQ ID NO:2. The cDNA of the antiviral protein Amarandin 1 derived from Amaranthus viridis has the signal peptide sequence in SEQ ID NO:3, MKMKKITNLVYILVAITTSVILQWTCNA in the 5-terminal. The cDNA of the antiviral protein Amarandin 2 derived from Amaranthus viridis has the signal peptide sequence in SEQ ID NO:3, MKMKKITNLVYILVAITTSVIFQWTCNA in the 5-terminal. A method for producing the antiviral protein Amarandin 1 comprises cloning cDNA of SEQ ID NO:1 into a hybrid vector pRSET, transforming Escherichia coli with the recombinant vector pRSET to prepare E. coli(KCTC-0344BP), and culturing E. coli(KCTC-0344BP). A method for producing the antiviral protein Amarandin 2 comprises cloning cDNA of SEQ ID NO:2 into a hybrid vector pRSET, transforming E. coli with the recombinant vector pRSET to prepare E. coli(KCTC-0345BP), and culturing E. coli(KCTC-0345BP).

5. 发明公开

KR1020020038995A 새로운 광역학 치료용 광감작제인9-하이드록시피오포바이드 에이 유도체 无效
标题翻译： 9-羟基磷酸酯衍生物作为新型光化学治疗中的光敏剂
公开(公告)号：KR1020020038995A
公开(公告)日：2002-05-25
申请号：KR1020000068864
申请日：2000-11-20
申请人： 금호석유화학 주식회사
发明人： 조정우 , 신은아 , 정창호 , 주영제 , 나경주 , 송필순 , 이호설 , 정필상 , 하만준
IPC分类号： C07D403/14
CPC分类号： C07D487/22 , A61K31/409 , A61K41/0071
摘要： PURPOSE: Provided is 9-hydroxypheophorbide-a derivative as photosensitizer in novel photodynamic therapy. The 9-hydroxypheophorbide-a derivative has more hydrophilicity than 10-hydroxypheophytin a and increased photodynamic therapy effects. CONSTITUTION: The 9-hydroxypheophorbide-a derivative of the formula(I) is manufactured by reducing carbonyl group at C9 of pheophytin a which is removed from metal ion of chlorophyll a then hydrolyzing ester group. In the formula, R1 and R2 are individually represent hydrogen, C1-6 linear or branched alkyl or C3-8 aryl, wherein R1 is preferably methyl and R2 is preferably hydrogen.
摘要翻译：目的：提供新型光动力疗法中9-羟基己糖醇衍生物作为光敏剂。 9-羟基异山梨醇衍生物具有比10-羟基鞘磷脂a更亲水性和增加的光动力疗法效果。构成：式（I）的9-羟基异山梨醇衍生物是通过还原从脱落于叶绿素a的金属离子然后水解酯基的脱镁叶绿素a的C9处的羰基还原制备的。在该式中，R 1和R 2各自独立地表示氢，C 1-6直链或支链烷基或C 3-8芳基，其中R 1优选为甲基，R 2优选为氢。

6. 发明公开

KR1020010066718A 식물 공변세포 특이적 유전자 발현 프로모터 失效
标题翻译：用于表达特定于植物保护细胞的基因的引物
公开(公告)号：KR1020010066718A
公开(公告)日：2001-07-11
申请号：KR1020000001144
申请日：2000-01-11
申请人： 금호석유화학 주식회사
发明人： 정창호 , 조정우 , 박선충 , 김용식
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12Y302/01031 , C12N9/2402 , C12N15/8225 , C12Y302/01028
摘要： PURPOSE: Provided is excellent promotor for the expression of a gene specific to a plant guard cell. And a vector including the promotor and a transformed plant cell with the vector are also provided. CONSTITUTION: A promotor for the expression of a DNA fragment specific to only a plant guard cell is composed of the base sequence represented by the sequence No. 1, wherein optional base can be deleted, inserted or transposed under maintaining the promotor activity and expression-regulation activity validly. The promotor is characterized in that it is isolated form Arabidopsis thaliana; it has a restriction sites for BstSFI, BamHI, BsawI, Hind III, XbaI, Bgl II, EcoRI, BsrDI and SmaI; and it has heat-shock reaction element, a pair of stress reaction elements and a nucleotide of 2.0 Kb.
摘要翻译：目的：提供对植物保护细胞特异性基因表达的优良启动子。还提供了包含启动子和具有载体的转化植物细胞的载体。构成：仅对植物保护细胞特异性的DNA片段的表达的启动子由序列号1所示的碱基序列组成，其中任选的碱基可以在维持启动子活性和表达 - 有效的监管活动。启动子的特征在于它是拟南芥的分离形式; 它具有BstSFI，BamHI，BsawI，Hind III，XbaI，Bgl II，EcoRI，BsrDI和SmaI的限制性位点; 并具有热冲击反应元件，一对应力反应元件和2.0Kb的核苷酸。

7. 发明授权

KR100387948B1 식물 공변세포 특이적 유전자 발현 프로모터 失效
标题翻译： 식물공변세포특이적유전자발현프로모터
公开(公告)号：KR100387948B1
公开(公告)日：2003-06-18
申请号：KR1020000001144
申请日：2000-01-11
申请人： 금호석유화학 주식회사
发明人： 정창호 , 조정우 , 박선충 , 김용식
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12Y302/01031 , C12N9/2402 , C12N15/8225 , C12Y302/01028
摘要： The present invention provides a noble promoter capable of specifically expressing desired gene in plant stomatal guard cells and a vector comprising the promoter. In addition, methods for generating an expression cassette, plasmids, and the use of the cassette and plasmids to produce transgenic plant cells and plants are described. The present invention can be utilized to drive guard cell-specific gene expression in plants for resistance to adverse weather conditions as well as guard cell-specific gene expression which influence growth of or yield from the plants.
摘要翻译：本发明提供了能够在植物气孔保卫细胞中特异性表达所需基因的贵重启动子和含有该启动子的载体。另外，描述了产生表达盒，质粒的方法，以及盒和质粒用于产生转基因植物细胞和植物的用途。本发明可用于驱动植物中的保卫细胞特异性基因表达以抵抗不利的天气条件以及影响植物生长或产量的保卫细胞特异性基因表达。

8. 发明授权

KR100356705B1 해바라기 파네실 피로인산 신타제 유전자를 갖는 식물벡터와그의 이용방법 失效
标题翻译：重组植物表达载体，其包含编码来自向日葵向日葵的幼苗的焦磷酸Farnesyl焦磷酸合酶FPS的分离的cDNA核苷酸序列
公开(公告)号：KR100356705B1
公开(公告)日：2002-10-19
申请号：KR1019990023823
申请日：1999-06-23
申请人： 금호석유화학 주식회사
发明人： 조정우 , 박선충 , 길현숙 , 정창호
IPC分类号： C12N15/82 , A01H5/00
摘要： 본발명은구조상다양한이소프레노이드생합성대사산물에대해중요한효소인해바라기파네실피로인산신타제를암호화하는분리된 cDNA의뉴클레오티드와펩티드서열을제공한다. 또한본 발명은상기뉴클레오티드서열을포함하는재조합식물발현벡터와유전자전환담배식물을생산하기위하여상기 DNA 서열을가진재조합식물발현벡터를도입하는숙주세포에대하여제공한다. 비유전자전환또는대조군벡터로형질전환된담배식물과비교할때 이종구조의파네실피로인산신타제를발현하는유전자전환식물은잎의클로로필고함량및 종자생산에있어서 2배의증가를나타낸다.

9. 发明公开

KR1020010003513A 해바라기 파네실 피로인산 신타제 유전자를 갖는 식물벡터와그의 이용방법 失效
标题翻译：含有CDNA核苷酸序列编码的重组植物表达载体富马酸磷酸酯合成酶（SFPS）和改造后的重组烟草植物
公开(公告)号：KR1020010003513A
公开(公告)日：2001-01-15
申请号：KR1019990023823
申请日：1999-06-23
申请人： 금호석유화학 주식회사
发明人： 조정우 , 박선충 , 길현숙 , 정창호
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8205 , A01H5/00 , C07K14/415 , C12N15/8271
摘要： PURPOSE: Provided is a recombinant plant expression vector containing cDNA nucleotide sequence coding sunflower farnesyl pyrophosphate synthase(SFPS) obtained from Helianthus annus. Also, a recombinant tobacco plant transformed with the same vector, is provided of which aging rate is delayed and seed production is improved. CONSTITUTION: The method for delaying aging of leaves of tobacco plant and for improving chlorophyll content, asexual reproduction, and seed production of tobacco plant is characterized by inserting a plant expression vector containing nucleic acid components and appropriate promoter into a cell of tobacco plant, in which the nucleic acid components contains the cDNA nucleotide sequence coding protein of sequence 1, the cDNA nucleotide sequence is isolated from the seed of Helianthus annus, and the plant expression vector is pCIP-sfps(KCTC 0520BP). The method for producing SFPS comprises the steps of: preparing the DNA fragments and followed by inserting into the host cell; incubating the transformed host cell in an appropriate medium using the SFPS gene to produce the title product.
摘要翻译：目的：提供含有从向日葵获得的向日葵法尼基焦磷酸合成酶（SFPS）的cDNA核苷酸序列的重组植物表达载体。此外，提供用相同载体转化的重组烟草植物，其老化速率延迟并且种子生产提高。构成：烟草植物叶片衰老和叶绿素含量延长，无性繁殖和种子生产的方法，其特征在于将含有核酸成分和合适促进剂的植物表达载体插入烟草植物细胞，核酸组分含有序列1的cDNA核苷酸序列编码蛋白，从向日葵的种子中分离cDNA核苷酸序列，植物表达载体是pCIP-sfps（KCTC 0520BP）。制备SFPS的方法包括以下步骤：制备DNA片段，然后插入宿主细胞; 使用SFPS基因在适当的培养基中培养转化的宿主细胞以产生标题产物。

10. 发明授权

KR100452431B1 강한 구조 유전자 발현을 위한 발현 카세트와 플라스미드 및 그의 사용방법 失效
标题翻译： 강한구조유전자발현을위위현현카세트와플라스드드및그의사용방강
公开(公告)号：KR100452431B1
公开(公告)日：2004-10-12
申请号：KR1020010021962
申请日：2001-04-24
申请人： 금호석유화학 주식회사
发明人： 정화지 , 김용식 , 조정우 , 정창호
IPC分类号： C12N15/11
摘要： PURPOSE: Provided are an expression cassette and a plasmid which have a strong structural promoter capable of binding to a heterologous gene for strong constitutive gene expression and their use. CONSTITUTION: A chimeric gene is characterized by containing a promoter and heterologous nucleic acid coding a desirable polypeptide, wherein the promoter is Arabidopsis trehalose-6-phosphate synthease 3 promoter of 1kb. In particular, the promoter is characterized by: (i) being isolated from Arabidopsis thaliana; having restriction enzyme sites for SacI(0.12kb), BspHI(0.17kb), SauI(0.78kb) and SalI(1.0kb); and containing a nucleotide sequence of SEQ ID NO:1.
摘要翻译：目的：提供一种具有强结构启动子的表达盒和质粒，所述结构启动子能够结合用于强组成型基因表达的异源基因及其用途。构成：嵌合基因的特征在于含有编码所需多肽的启动子和异源核酸，其中启动子是1kb的拟南芥海藻糖-6-磷酸合成酶3启动子。具体而言，启动子的特征在于：（i）从拟南芥中分离; 具有SacI（0.12kb），BspHI（0.17kb），SauI（0.78kb）和SalI（1.0kb）的限制酶切位点; 并含有SEQ ID NO：1的核苷酸序列。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式