专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

KR100266229B1 아마란터스 비리디스로 부터 아마란딘1과 아마란딘2의 항바이러스성 단백질 및 이를 코딩하는 디엔에이 失效
公开(公告)号：KR100266229B1
公开(公告)日：2004-05-20
申请号：KR1019970075883
申请日：1997-12-29
申请人： 금호석유화학 주식회사
发明人： 김용식 , 박선충 , 오수경 , 이호설 , 조정우 , 정창호
IPC分类号： C12N15/29
摘要： PURPOSE: Antiviral proteins Amarandin 1 and Amarandin 2 derived from Amaranthus viridis and cDNAs encoding the same proteins are provided, thereby isolating and sequencing the antiviral proteins Amarandin 1 and Amarandin 2, and producing a large quantity of antiviral proteins Amarandin 1 and Amarandin 2 using transformation technique. CONSTITUTION: The cDNA encoding the antiviral protein Amarandin 1 has the nucleotide sequence set forth in SEQ ID NO:1. The cDNA encoding the antiviral protein Amarandin 2 has the nucleotide sequence set forth in SEQ ID NO:2. The cDNA of the antiviral protein Amarandin 1 derived from Amaranthus viridis has the signal peptide sequence in SEQ ID NO:3, MKMKKITNLVYILVAITTSVILQWTCNA in the 5-terminal. The cDNA of the antiviral protein Amarandin 2 derived from Amaranthus viridis has the signal peptide sequence in SEQ ID NO:3, MKMKKITNLVYILVAITTSVIFQWTCNA in the 5-terminal. A method for producing the antiviral protein Amarandin 1 comprises cloning cDNA of SEQ ID NO:1 into a hybrid vector pRSET, transforming Escherichia coli with the recombinant vector pRSET to prepare E. coli(KCTC-0344BP), and culturing E. coli(KCTC-0344BP). A method for producing the antiviral protein Amarandin 2 comprises cloning cDNA of SEQ ID NO:2 into a hybrid vector pRSET, transforming E. coli with the recombinant vector pRSET to prepare E. coli(KCTC-0345BP), and culturing E. coli(KCTC-0345BP).

2. 发明公开

KR1020020038995A 새로운 광역학 치료용 광감작제인9-하이드록시피오포바이드 에이 유도체 无效
标题翻译： 9-羟基磷酸酯衍生物作为新型光化学治疗中的光敏剂
公开(公告)号：KR1020020038995A
公开(公告)日：2002-05-25
申请号：KR1020000068864
申请日：2000-11-20
申请人： 금호석유화학 주식회사
发明人： 조정우 , 신은아 , 정창호 , 주영제 , 나경주 , 송필순 , 이호설 , 정필상 , 하만준
IPC分类号： C07D403/14
CPC分类号： C07D487/22 , A61K31/409 , A61K41/0071
摘要： PURPOSE: Provided is 9-hydroxypheophorbide-a derivative as photosensitizer in novel photodynamic therapy. The 9-hydroxypheophorbide-a derivative has more hydrophilicity than 10-hydroxypheophytin a and increased photodynamic therapy effects. CONSTITUTION: The 9-hydroxypheophorbide-a derivative of the formula(I) is manufactured by reducing carbonyl group at C9 of pheophytin a which is removed from metal ion of chlorophyll a then hydrolyzing ester group. In the formula, R1 and R2 are individually represent hydrogen, C1-6 linear or branched alkyl or C3-8 aryl, wherein R1 is preferably methyl and R2 is preferably hydrogen.
摘要翻译：目的：提供新型光动力疗法中9-羟基己糖醇衍生物作为光敏剂。 9-羟基异山梨醇衍生物具有比10-羟基鞘磷脂a更亲水性和增加的光动力疗法效果。构成：式（I）的9-羟基异山梨醇衍生物是通过还原从脱落于叶绿素a的金属离子然后水解酯基的脱镁叶绿素a的C9处的羰基还原制备的。在该式中，R 1和R 2各自独立地表示氢，C 1-6直链或支链烷基或C 3-8芳基，其中R 1优选为甲基，R 2优选为氢。

3. 发明授权

KR100328507B1 나팔꽃 종자로부터 유래된 항균성 펩타이드의 ｃＤＮＡ를 포함하는 재조합 식물체 발현벡터 失效
标题翻译：重组植物表达载体，包含编码来自Pharbitis nil L.种子的抗菌肽的cDNA
公开(公告)号：KR100328507B1
公开(公告)日：2002-03-16
申请号：KR1019990006622
申请日：1999-02-27
申请人： 금호석유화학 주식회사
发明人： 구자춘 , 조무제 , 송필순 , 정창호
IPC分类号： C12N15/82 , A01H5/00
摘要： 본발명은다양한세균및 진균에대하여우수한항균활성을나타내어, 식물체에진균감염에대한내성을부여할수 있는, 나팔꽃() 종자에서유래한항균성펩타이드와이를코딩하는 cDNA, 전기 cDNA를포함하는재조합식물체발현벡터및 이를이용한항진균내성을가지는형질전이식물체(transgenic plant)의제조방법에관한것이다. 본발명의항균성펩타이드 Pn-AMP는다양한진균에대하여우수한항균활성을가지므로, 본발명의항진균내성을가지는형질전이식물체(transgenic plant)의제조방법에따라전기 Pn-AMP를코딩하는유전자를원하는식물세포에도입함으로써, 진균감염에대하여내성을가지는전이식물체를용이하게제조할수 있을것이다.

4. 发明公开

KR1020010066718A 식물 공변세포 특이적 유전자 발현 프로모터 失效
标题翻译：用于表达特定于植物保护细胞的基因的引物
公开(公告)号：KR1020010066718A
公开(公告)日：2001-07-11
申请号：KR1020000001144
申请日：2000-01-11
申请人： 금호석유화학 주식회사
发明人： 정창호 , 조정우 , 박선충 , 김용식
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12Y302/01031 , C12N9/2402 , C12N15/8225 , C12Y302/01028
摘要： PURPOSE: Provided is excellent promotor for the expression of a gene specific to a plant guard cell. And a vector including the promotor and a transformed plant cell with the vector are also provided. CONSTITUTION: A promotor for the expression of a DNA fragment specific to only a plant guard cell is composed of the base sequence represented by the sequence No. 1, wherein optional base can be deleted, inserted or transposed under maintaining the promotor activity and expression-regulation activity validly. The promotor is characterized in that it is isolated form Arabidopsis thaliana; it has a restriction sites for BstSFI, BamHI, BsawI, Hind III, XbaI, Bgl II, EcoRI, BsrDI and SmaI; and it has heat-shock reaction element, a pair of stress reaction elements and a nucleotide of 2.0 Kb.
摘要翻译：目的：提供对植物保护细胞特异性基因表达的优良启动子。还提供了包含启动子和具有载体的转化植物细胞的载体。构成：仅对植物保护细胞特异性的DNA片段的表达的启动子由序列号1所示的碱基序列组成，其中任选的碱基可以在维持启动子活性和表达 - 有效的监管活动。启动子的特征在于它是拟南芥的分离形式; 它具有BstSFI，BamHI，BsawI，Hind III，XbaI，Bgl II，EcoRI，BsrDI和SmaI的限制性位点; 并具有热冲击反应元件，一对应力反应元件和2.0Kb的核苷酸。

5. 发明公开

KR1019950026982A 신규한 비스페놀 알칸-분해균주 및 이를 이용한 비스페놀 알칸의 미생물학적 처리 방법 失效
标题翻译：一种新型双酚烷烃分解菌株及使用该菌株的双酚烷烃微生物处理方法
公开(公告)号：KR1019950026982A
公开(公告)日：1995-10-16
申请号：KR1019940006564
申请日：1994-03-30
申请人： 금호석유화학 주식회사
发明人： 김정일 , 이용훈 , 정창호
IPC分类号： C12N1/20

6. 发明授权

KR100452431B1 강한 구조 유전자 발현을 위한 발현 카세트와 플라스미드 및 그의 사용방법 失效
标题翻译： 강한구조유전자발현을위위현현카세트와플라스드드및그의사용방강
公开(公告)号：KR100452431B1
公开(公告)日：2004-10-12
申请号：KR1020010021962
申请日：2001-04-24
申请人： 금호석유화학 주식회사
发明人： 정화지 , 김용식 , 조정우 , 정창호
IPC分类号： C12N15/11
摘要： PURPOSE: Provided are an expression cassette and a plasmid which have a strong structural promoter capable of binding to a heterologous gene for strong constitutive gene expression and their use. CONSTITUTION: A chimeric gene is characterized by containing a promoter and heterologous nucleic acid coding a desirable polypeptide, wherein the promoter is Arabidopsis trehalose-6-phosphate synthease 3 promoter of 1kb. In particular, the promoter is characterized by: (i) being isolated from Arabidopsis thaliana; having restriction enzyme sites for SacI(0.12kb), BspHI(0.17kb), SauI(0.78kb) and SalI(1.0kb); and containing a nucleotide sequence of SEQ ID NO:1.
摘要翻译：目的：提供一种具有强结构启动子的表达盒和质粒，所述结构启动子能够结合用于强组成型基因表达的异源基因及其用途。构成：嵌合基因的特征在于含有编码所需多肽的启动子和异源核酸，其中启动子是1kb的拟南芥海藻糖-6-磷酸合成酶3启动子。具体而言，启动子的特征在于：（i）从拟南芥中分离; 具有SacI（0.12kb），BspHI（0.17kb），SauI（0.78kb）和SalI（1.0kb）的限制酶切位点; 并含有SEQ ID NO：1的核苷酸序列。

7. 发明授权

KR100343975B1 다중질병저항성을 나타내기 위하여 ＳＣａＭ4 또는 ＳＣａＭ5유 失效
标题翻译： 4 5具有多种SCaM4或SCaM5基因的转基因植物实现多种抗病性
公开(公告)号：KR100343975B1
公开(公告)日：2002-07-22
申请号：KR1019990006419
申请日：1999-02-26
申请人： 금호석유화학 주식회사
发明人： 허원도 , 조무제 , 송필순 , 정창호
IPC分类号： C12N5/04
摘要： 본발명은대두의칼모듈린이형태체(SCaM) 유전자들로형질전환되어광범위한식물병원체에대하여매우증진된저항성을나타내는유전자전환식물과그 식물세포를제공한다. 또한본 발명은병원체저항성식물을만들기위하여 SCaM 유전자들을가진발현벡터와발현벡터내의상기유전자들이도입된숙주세포를제공한다. 이종의 SCaM을발현하는유전자전환된식물은정상적으로는식물에침입하는진균류, 박테리아와바이러스들에대하여증가된저항성을나타낸다.

8. 发明授权

KR100328339B1 불친화적 상호 작용 중 높게 발현되는 작으면서시스테인이 많은 항균력을 갖고 있는 디펜신과 씨오닌유사 단백질 유전자들 失效
标题翻译：小和半胱氨酸丰富的抗真菌防御素和硫蛋白样蛋白基因在不相容的相互作用中高度表达
公开(公告)号：KR100328339B1
公开(公告)日：2002-03-12
申请号：KR1019990057802
申请日：1999-12-15
申请人： 금호석유화학 주식회사
发明人： 오병준 , 고문경 , 신병철 , 정창호
IPC分类号： C12N15/29
摘要： 본발명은(고추디펜신(defensin)단백질유전자)와(고추씨오닌(thionine) 유사단백질유전자)로명명되는 2개의 cDNA 클론과특유의구성요소즉, 코딩영역과유전자산물을포함하는부분; 그변이; 상기유전자, 코딩영역및 변이의적용; 유전자또는그 부분을각각함유하는 DNA 구조체, 벡터및 형질전환식물에관한것이다.

9. 发明公开

KR1020010056359A 불친화적 상호 작용 중 높게 발현되는 작으면서시스테인이 많은 항균력을 갖고 있는 디펜신과 씨오닌유사 단백질 유전자들 失效
标题翻译：具有抗菌活性和非亲和性相互作用高度表达的防御素和蛋白质PSEUDO蛋白
公开(公告)号：KR1020010056359A
公开(公告)日：2001-07-04
申请号：KR1019990057802
申请日：1999-12-15
申请人： 금호석유화학 주식회사
发明人： 오병준 , 고문경 , 신병철 , 정창호
IPC分类号： C12N15/29
CPC分类号： C12N15/8241 , C07K14/415
摘要： PURPOSE: Provided are PepDef and PepThi which have antibacterial effects and are useful in transforming plants in order for the plants to increase resistance against pathological bacteria. CONSTITUTION: PepDef DNA has 5-untranslated region of 9 bp and 3-untranslated region of 245 bp having poly(A) tail and one-open reading frame which codes 9.5kDa of polypeptide including 84 amino acids. PepThi DNA consists of 225bp excluding 5-untranslated region and 3-untranslated region having poly(A) tail and includes cDNA one-open reading frame which codes 8.5kDa of polypeptide having 75 amino acids. And the DNAs are obtained by the next steps of: i) cultivating Colletotrichum gloeosporioides in potato dextrose agar for 5 days at 27 deg.C; ii) obtaining RNA from the bacteria and manufacturing cDNA library; iii) sequencing DNA and performing RNA blotting and hybridization; and iv) cloning cDNA of thionine-like protein.
摘要翻译：目的：提供具有抗菌作用的PepDef和PepThi，可用于转化植物以使植物增加对病理细菌的抗性。构成：PepDef DNA具有9 bp的5个非翻译区和245 bp的3个非翻译区，具有poly（A）尾和一个开放阅读框，其编码9.5kDa的多肽，包括84个氨基酸。 PepThi DNA由不包括5-非翻译区的225bp和具有聚（A）尾的3-非翻译区组成，包括编码具有75个氨基酸的多肽的8.5kDa的cDNA一开放阅读框。通过以下步骤获得DNA：i）在马铃薯葡萄糖琼脂中培养5天，在27摄氏度培养; ii）从细菌获得RNA并制造cDNA文库; iii）测序DNA并进行RNA印迹和杂交; 和iv）克隆硫蛋白样蛋白质的cDNA。

10. 发明公开

KR1020000052262A 다중질병저항성을 나타내기 위하여 ＳＣａＭ4 또는 ＳＣａＭ5유 失效
标题翻译：具有SCAM4或SCAM5基因的基因转移植物显示多种抗病性和植物细胞
公开(公告)号：KR1020000052262A
公开(公告)日：2000-08-16
申请号：KR1019990006419
申请日：1999-02-26
申请人： 금호석유화학 주식회사
发明人： 허원도 , 조무제 , 송필순 , 정창호
IPC分类号： C12N5/04
CPC分类号： C12N5/04 , C12N15/8279
摘要： PURPOSE: A gene transferred plant which shows multi-diseases resistance to a wide range of pathogenic organisms of plant by being transferred to heterogeneous genes of soybean calmodulin(SCaM) such as SCaM4 or SCaM5 and plant cell thereof are provided. CONSTITUTION: A gene transferred plant, cells or offspring thereof express genes encoding SCaM4, which is deposited on KCTC(Korean Collection for Type Cultures) as a deposit number of 0543BP and has a sequence number of 1, or soybean calmodulin 5(SCaM5), which is deposited on KCTC as a deposit number of 0544BP and has a sequence number of 3. The multi-diseases resistance of higher plant life regarding one or more of the pathogenic organisms of plant is improved by transferring the gene transferred plant using two SCaM4 or SCaM5 gene vectors into the genes encoding SCaM4 or SCaM5.
摘要翻译：目的：提供通过转移到大豆钙调蛋白（SCaM）如SCaM4或其SCaM5及其植物细胞的异质基因，显示对广泛的植物致病生物多重抗病性的基因转移植物。构成：基因转移的植物，细胞或其后代表达编码SCaM4的基因，其存放于KCTC（Korean Collection for Type Cultures）上作为0543BP的保藏号，序列号为1或大豆钙调蛋白5（SCaM5），其沉积在KCTC上作为0544BP的沉积编号并具有序列号3.通过使用两个SCaM4转移基因转移的植物来改善植物生长对植物的一种或多种致病生物的更高植物寿命的多重疾病抗性或将SCaM5基因载体转入编码SCaM4或SCaM5的基因。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式