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    • 2. 发明公开
    • 균류를 이용한 키토산 생산방법
    • 从真菌生产壳聚糖的方法
    • KR1020030071389A
    • 2003-09-03
    • KR1020020011063
    • 2002-02-28
    • 강수태공청식경상대학교산학협력단
    • 강수태공청식오광수
    • C12P19/04
    • PURPOSE: A method for producing chitosan from fungi is provided, thereby simplifying the production process of chitosan, and decreasing the amount of waste water and waste matters. CONSTITUTION: A method for producing chitosan from fungi comprises the steps of: culturing Absidia coerulea IFO 5301 or Gongronella butleri IFO 8080 at pH 5.5 to 6.5 and 24 to 27 deg. C for 4 days and followed by culturing in a batch bioreactor at pH 5.5 to 6.5, 27 deg. C for 2 days with agitation of 600 to 650 rpm; filtering and washing with ethanol the cultured fungal mycelia, and freeze-drying the fungal mycelia; pulverizing the dried fungal mycelia and extracting them with 1N-NaOH at 121 deg. C for 15 minutes; filtering and washing with ethanol the alkali insoluble material and freeze-drying it; pulverizing the dried alkali insoluble materials(AIM) and treating it with acid; centrifuging the acid treated AIM and filtering the supernatant thereof; adding 20% NaOH into the filtered solution; and centrifuging, neutralizing and freeze-drying the precipitate to obtain fungal chitosan(FCS).
    • 目的:提供从真菌生产壳聚糖的方法,从而简化了壳聚糖的生产过程,减少了废水和废物的数量。 构成:从真菌制备壳聚糖的方法包括以下步骤:在pH5.5至6.5和24至27度下培养耻垢菌IFO 5301或Gongronella butleri IFO 8080。 培养4天,然后在分批生物反应器中在pH 5.5至6.5,27度下培养。 在600至650rpm的搅拌下搅拌2天; 用乙醇过滤和洗涤培养的真菌菌丝体,并冷冻干燥真菌菌丝体; 粉碎干燥的真菌菌丝体,并在121℃用1N-NaOH萃取。 C 15分钟; 用乙醇过滤和洗涤碱不溶物并冷冻干燥; 粉碎干燥的碱不溶物(AIM)并用酸处理; 离心酸处理的AIM并过滤其上清液; 向过滤的溶液中加入20%NaOH; 离心,中和冷冻干燥沉淀,得到真菌壳聚糖(FCS)。
    • 5. 发明公开
    • 굴 통조림 제조방법
    • 牡蛎罐的制备方法
    • KR1020020019119A
    • 2002-03-09
    • KR1020020009322
    • 2002-02-21
    • 공청식
    • 공청식
    • A23B4/00
    • A23B4/28A23V2002/00A23V2300/24
    • PURPOSE: Provided is a simple method of canning oyster by cleaning oyster, putting in a can with in-bamboo roasted salt, sealing and sterilizing. The method keeps taste and freshness of the oyster, making it to be stored for a long time. CONSTITUTION: The method of canning comprises: a step of eliminating unwanted materials by cooking the oyster at 100-110deg.C, separating the shell, cleaning the unwanted material on the surface by air and finally by hand; a sealing step of the can after adding to the above, 50-70wt.% of cleaned oyster, 20-10wt.% of in-bamboo roasted salt and 90-98wt.% of water, boiling, canning the oyster with the 30-50wt.% of the above boiled water and sealing; and a first sterilizing step of the above sealed can with high temperature steam and a second sterilizing step by the cooling water.
    • 目的:提供清洁牡蛎罐头牡蛎的简单方法,放入带竹内烤盐的罐头,密封消毒。 该方法保持牡蛎的口味和新鲜度,使其长时间储存​​。 构成:罐头方法包括:通过在100-110℃下烹饪牡蛎来消除不需要的物质的步骤,分离壳体,最后用手清洁表面上的不需要的物质; 在加入上述之后,罐头的密封步骤为50-70重量%的清洁牡蛎,20-10重量%的竹内烤盐和90-98重量%的水,沸腾,罐装牡蛎与30- 50重量%以上开水和密封; 和上述密封罐的第一消毒步骤,通过冷却水进行高温蒸汽和第二灭菌步骤。