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    • 9. 发明公开
    • 태반유래세포에 조건화된 배양 배지, 및 이를 이용한 무동물 및 무지지세포 줄기세포 배양 방법
    • 用于维持不同的干细胞的PLACENTA衍生细胞调控媒质和无动物的无饲养培养方法
    • KR1020120118407A
    • 2012-10-26
    • KR1020120016076
    • 2012-02-16
    • 고려대학교 산학협력단
    • 김병수이승진박용정지혜김지혜
    • C12N5/0735C12N5/02C12Q1/02
    • PURPOSE: A culture medium conditioned with placenta derivative cells, culturing method of animal-free and adactyl cell stem cell using the same and a manufacturing method of culture medium conditioned with placenta derivative cells are provided to prevent contamination problem caused by cells or xenoprotein. CONSTITUTION: A manufacturing method of culture medium conditioned with placenta derivative cells comprises the following steps: inoculating placenta derivative cells on gelatin-coated well plate; cultivating the placenta derivative cells bu addomg a cell broth; and collecting only the culture fluid. In the first step, the placenta derivative cell is placenta originated fibrous cell - similar cell separated from the human chorionic plate and is cultivated. In the second step, the cell broth is DMEM/F-12 which includes the blood serum substitute material. In the second step, the cultivating time is 20-30 hours. The stem cell culture medium is bFGF, IL-8, osteoprotegerin, uPAR, TIM-1, TIM-2, IGFBP-6, ICAM-1, angiogenin, BDNF, IGFBP-4, IGFBP-2, IL-6, GLP-2, MCP-1, GRO, or cytokine of GRO-a.
    • 目的:提供用胎盘衍生细胞调节的培养基,使用与胎盘衍生细胞调节的培养基的无动物培养方法和用于胎盘衍生细胞的培养基的制造方法,以防止由细胞或异种蛋白引起的污染问题。 构成:用胎盘衍生细胞调理的培养液的制造方法包括以下步骤:在明胶包被的孔板上接种胎盘衍生细胞; 培养胎盘衍生细胞,加入细胞培养液; 并仅收集培养液。 在第一步中,胎盘衍生细胞是胎盘起源的纤维细胞 - 类似细胞与人绒膜分离并培养。 在第二步中,细胞培养液是含有血清替代物质的DMEM / F-12。 第二步,栽培时间为20-30小时。 干细胞培养基是bFGF,IL-8,骨保护素,uPAR,TIM-1,TIM-2,IGFBP-6,ICAM-1,血管生成素,BDNF,IGFBP-4,IGFBP-2,IL-6, 2,MCP-1,GRO或GRO-α的细胞因子。