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    • 6. 发明公开
    • 인간 태반 유래 지지 세포를 이용한 인간 배아줄기세포의 배양 방법 및 그 배양 배지
    • 人类胚胎干细胞的培养方法使用人类衍生的饲养细胞及其培养基
    • KR1020110076251A
    • 2011-07-06
    • KR1020090132908
    • 2009-12-29
    • 고려대학교 산학협력단
    • 김병수박용최인영
    • C12N5/0735C12N5/02
    • PURPOSE: A method for culturing human embryonic stem cells using human placenta-derived feeder cells is provided to prevent interference of stem cell marker expression. CONSTITUTION: A method for culturing human embryonic stem cells comprises: a step of preparing human placenta-derived feeder cells(HPC); a step of transferring the human embryonic stem cells on a feeder cell; and a step of culturing the stem cells without fibroblast growth factor(FGF). The HPC is human placenta-derived fibroblast-like cells. The HPC is derived from chorionic plates. The FGF is a basic FGF(bFGF). A culture medium contains serum replacement(SR), mercaptoethanol, non-essential amino acid, and antibiotics.
    • 目的:提供使用人胎盘来源的饲养细胞培养人胚胎干细胞的方法,以防止干细胞标记物表达的干扰。 构成:培养人胚胎干细胞的方法包括:制备人胎盘来源的饲养细胞(HPC)的步骤; 将人胚胎干细胞转移到饲养细胞上的步骤; 以及培养没有成纤维细胞生长因子(FGF)的干细胞的步骤。 HPC是人胎盘来源的成纤维细胞样细胞。 HPC衍生自绒毛膜。 FGF是基本的FGF(bFGF)。 培养基含有血清替代物(SR),巯基乙醇,非必需氨基酸和抗生素。