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1. 发明公开

KR1020130052194A 난초류의 종자 형질전환 방법 失效
标题翻译：使用种子的转化方法
公开(公告)号：KR1020130052194A
公开(公告)日：2013-05-22
申请号：KR1020110117507
申请日：2011-11-11
申请人： 경상남도
发明人： 빈철구 , 김진기 , 김수경 , 노치웅 , 최복경 , 유재웅 , 김두환
IPC分类号： A01H5/10 , C12N15/82
摘要： PURPOSE: A method for transforming seeds of orchids is provided to shorten time for producing a transgenic plant without a process of inducing protocorm or a PLB(protocorm-like body). CONSTITUTION: A method for transforming seeds of orchids comprises a step of transfecting a seed with an agrobacterium containing a recombinant gene. Orchids are selected among Acianthus genus, Aerides genus, Anoectochilus genus, Ansellia genus, Aplectrum genus, Arethusa genus, Calanthe genus, Cattleya genus, Coelogyne genus, Corallorhiza genus, Cymbidium genus, Cypripedium genus, Crytopodium genus, Dendrobium genus, Epidendrum genus, Goodyera genus, Listera genus, Macodes genus, Oncidium genus, Phalaenopsis genus, Pholidota genus, Rhynchostylis genus, and Vanda genus. The seed is processed by disinfecting and cutting in a disk shape.
摘要翻译：目的：提供一种用于转化兰花种子的方法，以缩短生产转基因植物的时间，而不需要诱导原生质体或PLB（原生球体样体）的过程。构成：用于转化兰花种子的方法包括用含有重组基因的农杆菌转染种子的步骤。兰花属于贵阳属，气丝菌属，Anoectochilus属，Ansellia属，Aplectrum属，Arethusa属，Calanthe属，Cattleya属，Coelogyne属，Corallorhiza属，蕙兰属，Cypripedium属，Crytopodium属，石斛属，Epidendrum属，Goodyera 属，李斯特属属，Macodes属，Oncidium属，蝴蝶兰属，Pholidopsis属，Rhynchostylis属和Vanda属。种子通过消毒和切割成盘状进行处理。

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