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1. 发明公开

KR1020020009059A 테트라메틸 암모늄 하이드록사이드를 분해하는 신규한바실러스 세레우스 ＩＢＮ-Ｈ4 및 전기 균주를 이용한폐수처리방법 无效
标题翻译：用于分解三甲基氢氧化铵（TMAH）的新型巴豆酵母IBN-H4和使用菌株进行废水处理的方法
公开(公告)号：KR1020020009059A
公开(公告)日：2002-02-01
申请号：KR1020000042272
申请日：2000-07-22
申请人： (주)아이비진
发明人： 이대상 , 이미경 , 신철수 , 윤정환 , 염도영 , 이정기 , 박기돈 , 최호준 , 구본탁 , 강기정
IPC分类号： C12N1/20
摘要： PURPOSE: Novel bacillus cereus IBN-H4 (KCTC 0835BP) for decomposing trimethyl ammonium hydroxide (TMAH), which is useful for treating wastewater from etching process of silicon surface in semiconductor manufacturing facilities. CONSTITUTION: The bacillus cereus IBN-H4 (KCTC 0835BP) is shake-cultured in a culture medium containing (NH4)2SO4 3g/L, trace elements such as H3BO3 0.3g, CoSO 4·7H2O 0.2g, ZnSO4·H2O 0.1g, MnCl2·H2O 0.03g, NaMoO4·H2O 0.03g, NiCl2·H2O 0.02g, CuSO4·H2O 0.01g, FeSO4·H2O 20g, CaCl2·H2O 10g/L, MgSO4·7H2O(40%) 2mL/L, 1M phosphate buffer solution (pH 7), 1% TMAH acting as a carbon source at 30°C for 72hrs. Triple test (BOD) of the obtained culture solution show a BOD removal ratio higher than 90%. To culture the bacillus cereus IBN-H4 (KCTC 0835BP), such methods as batch culture, fed-batch culture, and continuous culture are preferably used.
摘要翻译：目的：分解三甲基氢氧化铵（TMAH）的新型蜡状芽孢杆菌IBN-H4（KCTC 0835BP），可用于处理半导体制造设施中硅表面蚀刻工艺的废水。构成：将含有（NH 4）2 SO 4 3g / L的培养基，H3BO3 0.3g，CoSO 4·7H 2 O 0.2g，ZnSO 4·H 2 O 0.1g等微量元素的培养基中的芽孢杆菌IBN-H4（KCTC 0835BP） MnCl2·H2O 0.03g，NaMoO4·H2O 0.03g，NiCl2·H2O 0.02g，CuSO4·H2O 0.01g，FeSO4·H2O 20g，CaCl2·H2O 10g / L，MgSO4·7H2O（40％）2mL / L，1M磷酸盐缓冲液溶液（pH7），1％TMAH作为碳源在30℃下72小时。所得培养液的三重试验（BOD）显示BOD去除率高于90％。为了培养蜡状芽孢杆菌IBN-H4（KCTC 0835BP），优选使用分批培养，补料分批培养和连续培养等方法。

2. 发明授权

KR100311758B1 다양한 고농도 유기성 폐기물의 반 연속 액상 발효를 통한 생물학적 전환 장치 및 공정 失效
标题翻译：使用半连续液体发酵的各种高浓度有机废物的生物转化装置和方法
公开(公告)号：KR100311758B1
公开(公告)日：2001-11-14
申请号：KR1019990017076
申请日：1999-05-13
申请人： (주)아이비진
发明人： 구본탁 , 박기돈 , 김진만 , 이정기 , 김국진 , 김현국
IPC分类号： B09B3/00
摘要： 본발명은고농도유기성폐기물의미생물발효를통한생물학적전환장치및 공정에관한것이고, 보다상세하게는고농도유기성폐기물을 1차발효를통하여표준기질화하고 2차발효를통해고부가가치물질을생산하는장치및 공정에관한것으로, 유기성폐기물로부터금속류, 비닐류, 유리류등을제거하기위한선별기(1), 수송관(2) 및파쇄기(3) 및전처리조(5)로구성되어있고, 전처리조에서는고농도유기성폐기물의고형분함량이 6∼8(w/v)%가되도록물을가하고습식멸균되어이후상온으로보관되며고농도유기성폐기물의혐기적발효를방지하기위하여산소공급및 교반시키고, 전처리조에서의액상유기물은수송관(7)을통해 1차발효조(8)로옮겨져카타볼라이트리프레션저항성변이주를이용하여발효시키고, 발효액은수송관(9)을거쳐원심분리기(10)로옮겨져고형분과상등액으로분리한후, 상등액의약 10∼20(v/v)%는 1차발효조내의효소농축을위해재순환(11)시키고, 초기발효에서상등액의나머지 80∼90(v/v)%는 2차발효조(12)로옮겨지고, 고형성분은수송관(13)을통해선택적으로건조기를통과한후 퇴비화나사료화공정에활용되어지고, 2차발효조(12)에서의발효는적용미생물에따라서유용한생성물을생성시키는장치및 그의활용방법에관한것이다.

3. 发明公开

KR1019990040825A 음식물쓰레기를이용한퇴비의제조방법 失效
标题翻译：利用食物垃圾制造堆肥的方法
公开(公告)号：KR1019990040825A
公开(公告)日：1999-06-05
申请号：KR1019970061310
申请日：1997-11-19
申请人： (주)아이비진
发明人： 이정기 , 최호석 , 구본탁 , 이일 , 김진만 , 최호준 , 김국진 , 박기돈
IPC分类号： C05F9/00
摘要： 본 발명은 대용량의 음식물 쓰레기를 호기성과 통성 혐기성의 미생물을 이용하여 발효시켜 퇴비를 제조하는 방법에 관한 것이다. 음식물 쓰레기를 원통 채분리기 및 수작업을 통하여 음식물 쓰레기에 포함된 이물질을 제거하고 압착 및 탈수과정을 통하여 전처리한 다음, 미생물 종균 및 부재료와 함께 로타리드럼 방식의 교반발효기에서 24 내지 48 시간 동안 발효하고, 밀폐식 및 정체식의 터널 발효기에서 다시 7 내지 21일간 발효하고 후숙하여 음식물 쓰레기의 양을 감소시키고 퇴비를 제조한다. 본 발명의 방법을 이용하면, 수 톤 내지 수십 톤 규모의 음식물 쓰레기를 효과적으로 처리하면서, 발효과정의 결과 우수한 품질의 퇴비를 제조할 수 있다.

4. 发明授权

KR100383375B1 파라티온 분해 미생물 버콜데리아 비에트나미엔시스 失效
标题翻译： 파라티온분해미생물버콜데리아비에트나미엔시스
公开(公告)号：KR100383375B1
公开(公告)日：2003-05-12
申请号：KR1020000051534
申请日：2000-09-01
申请人： (주)아이비진
发明人： 이정기 , 염도영 , 박기돈 , 이상준 , 이일 , 구본탁
IPC分类号： C12N1/20
摘要： PURPOSE: Provided is Burkholderia vietnamiensis which is isolated from the soil, excellently decomposes parathion and uses it as a carbon source, and is thus effectively used for the bioremediation. CONSTITUTION: Burkholderia vietnamiensisB-6 (KCTC 18041P) is isolated from the soil contaminated with parathion. It is excellent in decomposing parathion and grows using parathion as a carbon source. It can also be used for the bioremediation of waste-water containing parathion.
摘要翻译：用途：提供从土壤中分离出来的越南伯克霍尔德杆菌，极好地分解对硫磷并将其用作碳源，因此可有效地用于生物修复。组成：Burkholderia vietnamiensisB-6（KCTC 18041P）是从被对硫磷污染的土壤中分离出来的。用对硫磷作为碳源分解对硫磷和生长是极好的。它也可以用于含有对硫磷的废水的生物修复。

5. 发明授权

KR100291624B1 고온성 바실러스 써모글루코시데시어스의 유전자를 변이시킨 카타볼라이트 리프레션 내성 변이주 失效
标题翻译：使用遗传修饰技术的嗜热芽孢杆菌热葡萄糖苷酶的Catabolite抗性突变体
公开(公告)号：KR100291624B1
公开(公告)日：2001-05-15
申请号：KR1019990017130
申请日：1999-05-13
申请人： (주)아이비진
发明人： 구본탁 , 박기돈 , 김진만 , 이정기 , 김국진 , 김현국
IPC分类号： C12N1/20
摘要： 본발명은고온성바실러스써모글루코시데시어스(thermophilic Bacillus thermoglucosidasius)의유전자변이를통한카타볼라이트리프레션(catabolite repression) 내성변이주인전분분해효소생산변이주바실러스써모글루코시데시어스 BtgMu1(KFCC-11085호)와섬유소분해효소생산변이주바실러스써모글루코시데시어스 BtgcelMu1(KFCC-11084호)에관한것이다. 또한상기변이주를이용하여높은감량화능을필요로하는음식물쓰레기감량화로의응용과전분분해효소활성및 섬유소분해효소활성을현저히증가시키는전분분해효소특이적변이주(BtgMul)과섬유소분해효소특이적변이주(BtgcelMul)의동시배양(co-culture)에관한것이다.

6. 发明公开

KR1020020025396A 프로바이오틱 바실러스 메가테리움 ＰＳ352를 이용한 효소의 생산방법 失效
标题翻译：使用PROBIOTICS，BACILLUS MEGATERIUM PS352的酶制备方法
公开(公告)号：KR1020020025396A
公开(公告)日：2002-04-04
申请号：KR1020000057140
申请日：2000-09-28
申请人： (주)아이비진
发明人： 김현국 , 김국진 , 양선희 , 박기돈 , 염도영 , 이정기 , 최호준 , 구본탁
IPC分类号： C12N9/00
CPC分类号： C12N9/00 , A23K10/16 , C12R1/11
摘要： PURPOSE: Provided is an enzyme production method by solid fermentation using probiotics, Bacillus megaterium PS352. Also, provided is a use of the fermentate as an animal feedstuff of feed additives. CONSTITUTION: The enzyme production method is characterized by culturing Bacillus megaterium PS352 (KFCC-11179) in a mixed medium at 25-35 deg.C for 60-84 hours with controlling moisture content in the medium to 20-30% and providing 0.6-1.0vvm of dried air, wherein the enzyme is selected from the group consisting of amylase, protease, xylanase, pectinase, bata-glucanase and phytase. Th culture of Bacillus megaterium PS352 (KFCC-11179) can be used for feedstuffs and feed additives.
摘要翻译：目的：提供使用益生菌，巨大芽孢杆菌PS352进行固体发酵的酶生产方法。此外，提供了发酵物作为饲料添加剂的动物饲料的用途。构成：酶制造方法的特征在于在25-35℃的混合培养基中培养大枝芽孢杆菌PS352（KFCC-11179）60-84小时，将培养基中的水分含量控制在20-30％，并提供0.6- 1.0vvm的干燥空气，其中酶选自淀粉酶，蛋白酶，木聚糖酶，果胶酶，bata-葡聚糖酶和植酸酶。巨大芽孢杆菌PS352（KFCC-11179）的Th培养物可用于饲料和饲料添加剂。

7. 发明公开

KR1020020018286A 파라티온 분해 미생물 버콜데리아 비에트나미엔시스 失效
标题翻译： BURKHOLDERIA VIETNAMIENSIS DECOMPOSING PARATHION
公开(公告)号：KR1020020018286A
公开(公告)日：2002-03-08
申请号：KR1020000051534
申请日：2000-09-01
申请人： (주)아이비진
发明人： 이정기 , 염도영 , 박기돈 , 이상준 , 이일 , 구본탁
IPC分类号： C12N1/20
摘要： PURPOSE: Provided is Burkholderia vietnamiensis which is isolated from the soil, excellently decomposes parathion and uses it as a carbon source, and is thus effectively used for the bioremediation. CONSTITUTION: Burkholderia vietnamiensisB-6 (KCTC 18041P) is isolated from the soil contaminated with parathion. It is excellent in decomposing parathion and grows using parathion as a carbon source. It can also be used for the bioremediation of waste-water containing parathion.
摘要翻译：目的：提供从土壤中分离出的伯克霍尔德氏菌，极好地分解对硫磷并将其用作碳源，因此有效地用于生物修复。构成：将白僵菌B-6（KCTC 18041P）从受对硫磷污染的土壤中分离。它是分解对硫磷和生长使用对硫磷作为碳源是非常好的。也可用于含有对硫磷的废水的生物修复。

8. 发明授权

KR100437968B1 프로바이오틱 바실러스 메가테리움 ＰＳ352를 이용한 효소의 생산방법 失效
标题翻译： 프로바이오틱바실러스메가테움움PS352를이용한효소의생산방
公开(公告)号：KR100437968B1
公开(公告)日：2004-07-02
申请号：KR1020000057140
申请日：2000-09-28
申请人： (주)아이비진
发明人： 김현국 , 김국진 , 양선희 , 박기돈 , 염도영 , 이정기 , 최호준 , 구본탁
IPC分类号： C12N9/00
摘要： PURPOSE: Provided is an enzyme production method by solid fermentation using probiotics, Bacillus megaterium PS352. Also, provided is a use of the fermentate as an animal feedstuff of feed additives. CONSTITUTION: The enzyme production method is characterized by culturing Bacillus megaterium PS352 (KFCC-11179) in a mixed medium at 25-35 deg.C for 60-84 hours with controlling moisture content in the medium to 20-30% and providing 0.6-1.0vvm of dried air, wherein the enzyme is selected from the group consisting of amylase, protease, xylanase, pectinase, bata-glucanase and phytase. Th culture of Bacillus megaterium PS352 (KFCC-11179) can be used for feedstuffs and feed additives.
摘要翻译：目的：提供使用益生菌巨大芽孢杆菌PS352进行固体发酵的酶生产方法。此外，提供了发酵物作为饲料添加剂的动物饲料的用途。构成：酶制备方法的特征在于在25-35℃的混合培养基中培养巨大芽孢杆菌PS352（KFCC-11179）60-84小时，同时控制培养基中的水分含量为20-30％ 1.0vvm的干燥空气，其中酶选自淀粉酶，蛋白酶，木聚糖酶，果胶酶，β-葡聚糖酶和肌醇六磷酸酶。巨大芽孢杆菌PS352（KFCC-11179）的培养可用于饲料和饲料添加剂。

9. 发明公开

KR1020020009060A 테트라메틸 암모늄 하이드록사이드를 분해하는 신규한아시네토박터 속 ＩＢＮ-Ｈ7 및 전기 균주를 이용한폐수처리방법 无效
标题翻译：新款ACINETOBACTER SP 用于分解三甲基氢氧化铵（TMAH）的IBN-H7以及使用该应变的废水处理方法
公开(公告)号：KR1020020009060A
公开(公告)日：2002-02-01
申请号：KR1020000042273
申请日：2000-07-22
申请人： (주)아이비진
发明人： 이대상 , 이미경 , 신철수 , 윤정환 , 염도영 , 이정기 , 박기돈 , 최호준 , 구본탁 , 강기정
IPC分类号： C12N1/20
摘要： PURPOSE: Novel acinetobacter sp. IBN-H7 (KCTC 0836BP) for decomposing trimethyl ammonium hydroxide (TMAH), which is useful for treating wastewater from etching process of silicon surface in semiconductor manufacturing facilities. CONSTITUTION: The acinetobacter sp. IBN-H7 (KCTC 0836BP) is shake-cultured in a culture medium containing (NH4)2SO4 3g/L, trace elements such as H3BO3 0.3g, CoSO 4·7H2O 0.2g, ZnSO4·H2O 0.1g, MnCl2·H2O 0.03g, NaMoO4·H2O 0.03g, NiCl2·H2O 0.02g, CuSO4·H2O 0.01g, FeSO4·H2O 20g, CaCl2·H2O 10g/L, MgSO4·7H2O(40%) 2mL/L, 1M phosphate buffer solution (pH 7), 1% TMAH acting as a carbon source at 30°C for 72hrs. Triple test (BOD) of the obtained culture solution show a BOD removal ratio higher than 90%. To culture the acinetobacter sp. IBN-H7 (KCTC 0836BP), such methods as batch culture, fed-batch culture, and continuous culture are preferably used. In the wastewater treatment method, one strain selected from kluvyeromyces delphinesis IBN-H1(KCTC 0834BP) or bacillus cereus IBN-H4(KCTC 0835BP) is added.
摘要翻译：目的：新型不动杆菌属用于分解三甲基氢氧化铵（TMAH）的IBN-H7（KCTC 0836BP），其可用于处理半导体制造设施中硅表面的蚀刻工艺的废水。构成：不动杆菌属将IBN-H7（KCTC 0836BP）在含有（NH 4）2 SO 4 3g / L，痕量元素如H3BO3 0.3g，CoSO 4·7H 2 O 0.2g，ZnSO 4·H 2 O 0.1g，MnCl 2·H 2 O 0.03g ，NaMoO4·H2O 0.03g，NiCl2·H2O 0.02g，CuSO4·H2O 0.01g，FeSO4·H2O 20g，CaCl2·H2O 10g / L，MgSO4·7H2O（40％）2mL / L，1M磷酸盐缓冲溶液（pH7），1％TMAH在30℃下作为碳源72小时。所得培养液的三重试验（BOD）显示BOD去除率高于90％。培养不动杆菌属 IBN-H7（KCTC 0836BP）优选使用分批培养，补料分批培养和连续培养等方法。在废水处理方法中，加入了选自德氏梭菌IBN-H1（KCTC 0834BP）或蜡状芽孢杆菌IBN-H4（KCTC 0835BP）的一种菌株。

10. 发明公开

KR1020020008356A 테트라메틸 암모늄 하이드록사이드를 분해하는 신규한클루이베로마이세스 델펜시스 ＩＢＮ-Ｈ1 및 전기 균주를이용한 폐수처리방법 无效
标题翻译：新型KLUYVEROMYCES DELPHENSIS IBN-H1分解四甲基氢氧化铵和使用该方法处理废水的方法
公开(公告)号：KR1020020008356A
公开(公告)日：2002-01-30
申请号：KR1020000042271
申请日：2000-07-22
申请人： (주)아이비진
发明人： 이대상 , 이미경 , 신철수 , 윤정환 , 염도영 , 이정기 , 박기돈 , 최호준 , 구본탁 , 강기정
IPC分类号： C12N1/16
摘要： PURPOSE: Provided are novel Kluyveromyces delphensis IBN-H1 decomposing tetramethyl ammonium hydroxide (TMAH) and a method for treating waste water using the same strain. TMAH is used in etching the surface of the silicon, and is accordingly contained in the waste water of a semiconductor plant. It is hardly decomposed and shows toxicity. This waste water containing TMAH is treated by the above novel strain eliminating 90% of TMAH therefrom. The process can be used in the industry as an environment-friendly waste water treatment. CONSTITUTION: The strain is prepared by: culturing strains in the waste water of semiconductor plants and culturing again the above strains in the medium containing TMAH; selecting 7 strains showing resistance to TMAH; and naming them as IBN H- 1-IBN H-7 and choosing IBN H-1, IBN H-4 and IBN H-7, which have excellent TMAH decomposability. They are identified as a novel strain belonging to Kluyveromyces delphensis, Bacillus cereus and Acinetobacter sp. by BIOLOG system, MIDI and partial base sequence of 16s RNA and named Kluyveromyces delphensis IBN H-1 (KCTC 0834BP), Bacillus cereus IBN H-4 (KCTC 0835BP) and Acinetobacter sp. IBN H-7 (KCTC 0836PB). The biological treatment of the waste water with TMAH is carried out by a batch culture, more preferably fed-batch culture or continuous culture.
摘要翻译：目的：提供新型克氏疏螺旋体IBN-H1分解四甲基氢氧化铵（TMAH）和使用相同菌株处理废水的方法。 TMAH用于蚀刻硅的表面，因此被包含在半导体工厂的废水中。它几乎不分解并显示毒性。含有TMAH的废水由上述新型菌株处理，消除了90％的TMAH。该工艺可作为环保废水处理在工业中使用。构成：菌株通过：在半导体植物的废水中培养菌株，并在含有TMAH的培养基中再次培养上述菌株; 选择显示耐TMAH的7株; 并命名为IBN H-1-IBN H-7，并选择具有优异TMAH分解性的IBN H-1，IBN H-4和IBN H-7。它们被鉴定为属于德国克鲁维酵母（Kluyveromyces delphensis），蜡样芽胞杆菌（Bacillus cereus）和不动杆菌属（Acinetobacter sp。）的新菌株。通过BIOLOG系统，16S RNA的MIDI和部分碱基序列，命名为Kluyveromyces delphensis IBN H-1（KCTC 0834BP），蜡状芽孢杆菌IBN H-4（KCTC 0835BP）和不动杆菌属。 IBN H-7（KCTC 0836PB）。使用TMAH对废水的生物处理通过分批培养进行，更优选补料分批培养或连续培养。

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