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1. 发明公开

KR1020070029126A 단백질 해석 방법 无效
标题翻译：蛋白质分析方法
公开(公告)号：KR1020070029126A
公开(公告)日：2007-03-13
申请号：KR1020067014203
申请日：2004-12-17
申请人： 야마우치 요시오 , 신카와 다카시 , 이소베 도시아키
发明人： 야마우치요시오 , 신카와다카시 , 이소베도시아키
IPC分类号： G01N33/68 , G01N33/48 , C12Q1/37 , G06F19/18
CPC分类号： G01N33/6848 , G01N33/6818
摘要： It is intended to provide a protein analysis method whereby proteins can be identified and the quantification data can be obtained by a convenient procedure. Namely, a protein analysis method characterized by comprising: the step of cleaving two protein-containing samples individually with restriction enzymes at specific amino acid sites to give peptide chain-containing samples; the step of modifying peptide chains contained in the peptide chain- containing samples with labeling compounds having different masses due to isotopes so as to impart different masses to the peptide chains; the step of mixing the isotope-labeled peptide chain-containing samples and fractionally quantifying the sample mixture for individual peptide chains so as to determine a content ratio; the step of selecting peptide chains to be subjected to amino acid sequence identification from the peptide chains and identifying the amino acid sequences of the peptide chains; the step of specifying proteins corresponding to the peptide chains; and the step of determining the content ratio of the thus specified proteins based on the fractional quantification data of the peptide chains. ® KIPO & WIPO 2007
摘要翻译：旨在提供蛋白质分析方法，其中可以鉴定蛋白质，并且可以通过方便的方法获得定量数据。即，蛋白质分析方法，其特征在于，包括：在特定氨基酸位点用限制性内切酶单独裂解两个含蛋白质的样品，得到含有肽链的样品的步骤; 利用由于同位素而具有不同质量的标记化合物来修饰含有肽链的样品中含有肽链的步骤，以赋予肽链不同的质量; 混合同位素标记的含有肽链的样品并对各个肽链的样品混合物进行分数定量以确定含量比的步骤; 从肽链中选择要进行氨基酸序列鉴定的肽链并鉴定肽链的氨基酸序列的步骤; 指定对应于肽链的蛋白质的步骤; 以及基于肽链的分数定量数据确定如此指定的蛋白质的含量比的步骤。 ®KIPO＆WIPO 2007

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