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    • 70. 发明公开
    • 소 복제 수정란의 체외배양 방법
    • 在体育文化中改善基因组织胚胎
    • KR1020110050100A
    • 2011-05-13
    • KR1020090106944
    • 2009-11-06
    • 충북대학교 산학협력단
    • 김남형추이시앙쑨쉬융난센싱후이
    • C12N5/07C12N5/02
    • PURPOSE: A method for in vitro culture of bovine cloned fertilized eggs is provided to enhance cell generation rate. CONSTITUTION: A method for in vitro culture of bovine cloned fertilized eggs comprises: a step of preparing a culture medium in stock form and storing in a refrigerator or freezer; a step of treating 50nM of trichostatin A to the basic culture medium under the presence of 0.4% BSA(bovine serum albumin); a step of culturing the bovine cloned fertilized eggs in vitro; a step of isolating cells from bovine ear and establishing somatic cell line; a step of removing cumulus cells using hyaluronidase for enucleation; a step of pricking the zona pellucida using a enucleation pipette and inhaling cytoplasm and first polar body; a step of transplanting a donor cell to the enucleated egg; a step of injecting one donor cell; and a step of fusing and activating.
    • 目的:提供牛克隆受精卵的体外培养方法,以提高细胞产生率。 构成:牛克隆受精卵的体外培养方法包括:制备储备形式的培养基并储存在冰箱或冷冻机中的步骤; 在0.4%BSA(牛血清白蛋白)存在下将50nM曲古抑菌素A处理至碱性培养基的步骤; 体外培养牛克隆受精卵的步骤; 从牛耳分离细胞并建立体细胞系的步骤; 使用透明质酸酶去除卵丘细胞进行摘除的步骤; 使用去核移液管穿刺透明带并吸入细胞质和第一极体的步骤; 将供体细胞移植到去核卵的步骤; 注入一个供体细胞的步骤; 和融合和激活的一个步骤。