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    • 61. 发明公开
    • 황기로부터 칼리코신을 분리 및 정제하는 방법
    • 一种从ASTRAGALI RADIX中分离和纯化钙蛋白的方法
    • KR1020080079237A
    • 2008-08-29
    • KR1020080081310
    • 2008-08-20
    • 주식회사 사임당화장품최상원
    • 윤경섭임경란최상원
    • A61K36/481C07D311/36
    • A61K36/481A61K2236/333C07D311/36C12N9/24C12N9/48
    • A method for isolating and purifying calycosin from Astragali Radix is provided to isolate and purify the calycosin effectively, which is an isoflavone ingredient of phytoestrogen and shows anti-cancer, anti-hypertension, anti-osteoporosis and anti-aging activities, from the Astragali Radix. A method for isolating and purifying calycosin from Astragali Radix comprises the steps of: (a) after extracting Astragali Radix or crushed Astragali Radix with C1-4 alcohol or an aqueous solution of the alcohol, fractionating an extract of the Astragali Radix with an organic solvent for fat-removing such as n-hexane, thereby removing oil from an upper layer; (b) treating the fat-removed extract with carbohydrase and pectinase; (c) adding ethyl acetate to a product obtained from the step(b) to obtain an ethyl acetate fraction; (d) isolating or purifying the fraction of the step(c) using absorption ion resin column chromatography; and (e) eluting the calycosin absorbed absorption ion resin column using an aqueous solution of ethanol having the ethanol content of 60-100 wt.% or ethanol.
    • 提供了从黄芪中分离和纯化胡萝卜素的方法,用于有效分离和纯化赤藓红素,其是植物雌激素的异黄酮成分,并显示抗黄芪药物的抗癌,抗高血压,抗骨质疏松和抗衰老活性 。 从黄芪中分离和纯化胡萝卜素的方法包括以下步骤:(a)用C1-4醇或醇水溶液萃取黄芪或粉碎的黄芪后,用有机溶剂分馏黄芪提取物 用于脂肪去除如正己烷,从而从上层除去油; (b)用碳水解酶和果胶酶处理除去脂肪的提取物; (c)向步骤(b)得到的产物中加入乙酸乙酯,得到乙酸乙酯级分; (d)使用吸收离子树脂柱色谱法分离或纯化步骤(c)的级分; 和(e)使用乙醇含量为60-100重量%的乙醇的水溶液或乙醇洗脱所述卡洛糖苷吸收的离子树脂柱。
    • 62. 发明公开
    • 호열성 서머스 균주로부터 분리된 신규한 내열성 알파-글루코시다제 및 그 제조방법
    • 热稳定剂热稳定性阿尔法 - 胶原酶,热钙GK24及其制备方法
    • KR1020010035734A
    • 2001-05-07
    • KR1019990042431
    • 1999-10-01
    • 한국생명공학연구원
    • 이대실나시루고석훈고정헌김미경김중수
    • C12N9/24
    • C12N9/24C12N15/09C12Y302/0102
    • PURPOSE: Provided is a method for manufacturing heat-resistant alpha-glucosidase which acts as a catalyst in the process of increasing the yield of glucose by alpha-amylase and which is very useful in producing new polysaccharide in food and medical industries. CONSTITUTION: A method for manufacturing heat-resistant alpha-glucosidase comprises the following steps of: i) centrifuging the broth of Thermus caldophilus GK24 and storing the cells under cold condition; ii) destroying and centrifuging the cells to obtain co-enzymes, which are added with ammonium sulfate to manufacture enzyme solution; iii) selecting and purifying enzymes from the solution through column chromatography; iv) determining the molecular weight of the purified alpha-glucosidase by SDS polyacryl amide gel electrophoresis; v) reacting the purified alpha-glucosidase with isomaltose and various ions to measure proper temperature for activation; vi) identifying sugars reacting with the purified alpha-glucosidase and determining N-terminal sequence of the protein; vii) synthesizing DNA probe based on the amino acid sequence of the enzyme and manufacturing DNA of the strain; viii) determining the base sequence of a gene from DNA segments having the gene of the purified alpha-glucosidase; ix) manufacturing recombinant E. coli by using a plasmid vector containing the gene; and x) cultivating the transformant to obtain alpha-glucosidase and analyzing the products of the bacteria by reacting enzymes with other sugars.
    • 目的:提供一种在通过α-淀粉酶提高葡萄糖产率的过程中作为催化剂的耐热α-葡萄糖苷酶的制备方法,其在食品和医疗行业生产新型多糖中非常有用。 构成:制备耐热α-葡萄糖苷酶的方法包括以下步骤:i)将嗜热栖热菌GK24的培养液离心并在冷条件下储存细胞; ii)破坏和离心细胞以获得辅酶,加入硫酸铵以制备酶溶液; iii)通过柱色谱法从溶液中选择和纯化酶; iv)通过SDS聚丙烯酰胺凝胶电泳测定纯化的α-葡萄糖苷酶的分子量; v)使纯化的α-葡糖苷酶与异麦芽糖和各种离子反应以测量适当的活化温度; vi)鉴定与纯化的α-葡糖苷酶反应并确定蛋白质的N末端序列的糖; vii)根据酶的氨基酸序列合成DNA探针并制备该菌株的DNA; viii)确定具有纯化的α-葡糖苷酶基因的DNA区段的基因的碱基序列; ix)通过使用含有该基因的质粒载体制备重组大肠杆菌; 和x)培养转化体以获得α-葡糖苷酶,并通过酶与其他糖反应来分析细菌的产物。
    • 68. 发明公开
    • 베타-아가레이즈, 카파-카라기나아제 및 무수갈락토시다아제를 함유하는 효소 복합체 및 그 용도
    • 含有β-琼脂糖酶,κ-卡拉胶酶和无水半乳糖苷酶的酶复合物及其用途
    • KR1020170075303A
    • 2017-07-03
    • KR1020150184782
    • 2015-12-23
    • 고려대학교 산학협력단
    • 한성옥강대희현정은
    • C12N9/24C12N15/52C12N15/63C07K14/33
    • C07K14/33C12N9/24C12N15/52C12N15/63
    • 본발명은키메릭카파-카라기나아제및 키메릭베타-아가레이즈및 키메릭무수갈락토시다아제를함유하는효소복합체에관한것으로, 더욱자세하게는베타-아가레이즈와엔도-β-1,4-글루카나아제-B의도커린모듈이융합된키메릭베타-아가레이즈, 카파-카라기나아제와엔도-β-1,4-글루카나아제-B의도커린모듈이융합된키메릭카파-카라기나아제, 무수갈락토시다아제와엔도-β-1,4-글루카나아제-B의도커린모듈이융합된키메릭무수갈락토시다아제및 소형셀룰로즈-결합단백질 A가결합되어있는효소복합체및 이를이용한홍조류바이오매스의분해방법에관한것이다. 본발명에따르면, 아가분해생산물제조에있어서물리적, 화학적전처리공정에의존하였던기존의방식에서엔자임을이용한분해공정을도입함으로써뛰어난효율성, 생산물의제어, 간편한활용성, 저비용고효율의친환경적분해시스템을이용하여해양조류부터가치있는생산물을효율적으로전환하는데크게기여할것으로기대된다.
    • 本发明嵌合的卡伯色机或脱水酶和嵌合的β-琼脂糖酶和嵌合无水半乳糖苷酶涉及一种酶复合物包含脱水酶,并且更具体的β-琼脂糖酶和Yen图-β-1,4- - 葡聚糖酶-B旨在keorin模块融合嵌合的β-琼脂糖酶,κ色机或-β-1,4-脱水酶和图日元葡聚糖酶-B旨在keorin模块融合嵌合卡伯卡拉 其中嵌合无水半乳糖苷酶和小纤维素结合蛋白A融合的酶复合物和内切-β-1,4-葡聚糖酶 - 以及使用该方法分解藻类生物质的方法。 根据本发明,通过使用以常规方式谁取决于物理和化学预处理工艺在生产酶,产品的控制,方便使用性的引入分解过程中,采用成本低,效率高的环境友好的分解系统的琼脂分解产物优异的效率 预计这将大大有助于从海藻中有效转化有价值的产品。