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    • 10. 发明公开
    • 홍화씨 유래의 세로토닌 유도체, 리그난 및 플라보노이드 성분의 분리 및 정제 방법
    • 从高效液相色谱法(CARTHAMUS TINCTORIUS L.)中分离和纯化丝氨酸,LIGNANS和FLAVONOIDS的方法,以高效率生产丝氨酸衍生物和酚醛
    • KR1020040084353A
    • 2004-10-06
    • KR1020030019357
    • 2003-03-28
    • 최상원김종성
    • 최상원김종성
    • A61K36/286A61K8/97A23L7/10
    • C12P17/10B01D15/38C12P5/005C12P17/06C12Y302/01
    • PURPOSE: A method for isolation and purification of serotonins, lignans and flavonoids from safflower(Carthamus tinctorius L.) seeds is provided, thereby mass producing serotonin derivatives and phenols from safflower seeds with high work efficiency. CONSTITUTION: The method for isolation and purification of serotonins, lignans and flavonoids from safflower(Carthamus tinctorius L.) seeds comprises the steps of: roasting dried safflower seeds, and removing fat from the roasted safflower seeds by using an oil press or by adding 100% normal-hexane into the safflower seeds and heating the mixture for 2 hours; adding 80% water-soluble ethanol into the fat-removed safflower seeds, heating the mixture at 70 to 90 deg. C, and concentrating the extract under reduced pressure; treating the concentrate with hot water, and adding pectinase into the hot water treated concentrate to hydrolyze it; and passing the hydrolyzed product through the Diaion HP-20 resin, eluting the resin with 60% to 100% water-soluble ethanol, and subjecting the eluate to Sephadex LH-20 column chromatography.
    • 目的:提供红花(Carthamus tinctorius L.)种子中血清素,木脂素和黄酮类化合物的分离纯化方法,从而大量生产来自红花种子的5-羟色胺衍生物和酚类,工作效率高。 构成:从红花(Carthamus tinctorius L.)种子中分离纯化血清素,木脂素和类黄酮的方法包括以下步骤:烘干干红花种子,并通过使用油压机或通过加入100来从焙炒的红花种子中除去脂肪 将正己烷加入红花种子中并加热混合物2小时; 向除去脂肪的红花种子中加入80%的水溶性乙醇,将混合物加热至70〜90℃。 并在减压下浓缩提取物; 用热水处理浓缩物,并将果胶酶加入到热水处理的浓缩物中以水解; 并将水解产物通过Diaion HP-20树脂,用60%至100%的水溶性乙醇洗脱树脂,并使洗脱液进行Sephadex LH-20柱色谱。