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    • 23. 发明公开
    • 효소반응을 이용한 글루콘산 칼슘의 제조방법
    • 使用酶反应生产葡萄糖酸钙的方法
    • KR1020030096457A
    • 2003-12-31
    • KR1020020032725
    • 2002-06-12
    • 주식회사 한바이오텍
    • 김정수
    • C12P7/58
    • PURPOSE: A method for producing calcium gluconate using enzyme reaction is provided. The production of calcium gluconate is carried out under mild condition and oxygen which is produced from the enzyme reaction in oxidation of glucose is reused. Therefore, the production costs of calcium gluconate can be decreased. CONSTITUTION: A method for producing calcium gluconate using enzyme reaction comprises the steps of: oxidizing glucose by the reaction of glucose oxidase and catalase under the atmospheric pressure and oxygen concentration of 0.2 to 0.8 vvm; and adding a pH controlling agent and calcium carbonate into the oxidized glucose, wherein the reaction temperature is 30 to 40 deg. C and the reaction pH is 5.0 to 8.0; the concentration of glucose is 20 to 50%; and the concentration of glucose oxidase or catalase relative to the concentration of glucose is 0.5 to 3.0%.
    • 目的:提供使用酶反应生产葡萄糖酸钙的方法。 葡萄糖酸钙的生产在温和条件下进行,并且由葡萄糖氧化中的酶反应产生的氧被再利用。 因此,可以降低葡萄糖酸钙的生产成本。 构成:使用酶反应生产葡萄糖酸钙的方法包括以下步骤:通过葡萄糖氧化酶和过氧化氢酶在大气压和氧浓度为0.2至0.8vvm下的反应来氧化葡萄糖; 并向所述氧化葡萄糖中加入pH控制剂和碳酸钙,其中所述反应温度为30〜40℃。 C,反应pH为5.0〜8.0; 葡萄糖浓度为20〜50%; 葡萄糖氧化酶或过氧化氢酶的浓度相对于葡萄糖浓度为0.5〜3.0%。
    • 24. 发明公开
    • 글루콘산 제일철의 제조방법
    • 制备富含葡萄糖的方法
    • KR1020030035140A
    • 2003-05-09
    • KR1020010067070
    • 2001-10-30
    • 주식회사 한바이오텍
    • 김정수
    • C12P7/58
    • PURPOSE: A process for preparing ferrous gluconate is provided, thereby the high purity of ferrous gluconate can be directly and cheaply prepared using glucose oxidase and catalase. CONSTITUTION: The process for preparing ferrous gluconate comprises the steps of: oxidation of glucose using glucose oxidase and catalase enzyme reaction in a reactor under the aeration rate of 0.2 to 0.8 vvm; and adding ferrous carbonate(FeCO3) as a pH regulator and a ferrous source into the oxidized glucose, wherein the temperature of the reactor is 25 to 35 deg. C and pH of the reactor is 6.0 to 7.0; the concentration of glucose is 10 to 40%; the concentration of glucose oxidase to glucose is 0.5 to 3.0%; the concentration of catalase to glucose is 0.5 to 3.0%; and oxygen produced by the reaction of oxidized glucose and ferrous carbonate is reused in the oxidation of glucose.
    • 目的:提供葡萄糖酸亚铁制备方法,可以使用葡萄糖氧化酶和过氧化氢酶直接廉价地制备高纯度的葡萄糖酸亚铁。 构成:制备葡萄糖酸亚铁的方法包括以下步骤:在0.2至0.8vvm的通气速率下,用反应器中的葡萄糖氧化酶和过氧化氢酶反应氧化葡萄糖; 并将碳酸亚铁(FeCO 3)作为pH调节剂和亚铁源加入到氧化葡萄糖中,其中反应器的温度为25至35℃。 反应器的C和pH为6.0-7.0; 葡萄糖浓度为10〜40%; 葡萄糖氧化酶对葡萄糖的浓度为0.5〜3.0%; 过氧化氢酶对葡萄糖的浓度为0.5〜3.0%; 氧化葡萄糖和碳酸亚铁反应产生的氧气被重新用于葡萄糖的氧化。
    • 25. 发明公开
    • 효소반응을 이용한 글루콘산 마그네슘의 제조방법
    • 使用酶反应制备葡萄糖酸镁的方法
    • KR1020030035139A
    • 2003-05-09
    • KR1020010067069
    • 2001-10-30
    • 김정수
    • 김정수
    • C12P7/58
    • PURPOSE: A process for preparing magnesium gluconate using enzyme reaction is provided, thereby the high purity of magnesium gluconate can be directly and cheaply prepared using glucose oxidase and catalase. CONSTITUTION: The process for preparing magnesium gluconate comprises the steps of: oxidation of glucose using glucose oxidase and catalase enzyme reaction in a reactor under the aeration rate of 0.2 to 0.8 vvm; and adding magnesium hydroxide as a pH regulator and a ferrous source into the oxidized glucose, wherein the temperature of the reactor is 25 to 35 deg. C and pH of the reactor is 5.0 to 8.0; the concentration of glucose is 10 to 50%; the concentration of glucose oxidase to glucose is 0.5 to 3.0%; the concentration of catalase to glucose is 0.5 to 3.0%; and oxygen produced by the reaction of oxidized glucose and magnesium hydroxide is reused in the oxidation of glucose.
    • 目的:提供使用酶反应制备葡萄糖酸镁的方法,从而可以使用葡萄糖氧化酶和过氧化氢酶直接和廉价地制备高纯度的葡萄糖酸镁。 构成:制备葡萄糖酸镁的方法包括以下步骤:在0.2至0.8vvm的通气速率下,用反应器中的葡萄糖氧化酶和过氧化氢酶反应氧化葡萄糖; 并将氢氧化镁作为pH调节剂和亚铁源加入到氧化葡萄糖中,其中反应器的温度为25至35℃。 反应器的C和pH为5.0-8.0; 葡萄糖浓度为10〜50%; 葡萄糖氧化酶对葡萄糖的浓度为0.5〜3.0%; 过氧化氢酶对葡萄糖的浓度为0.5〜3.0%; 通过氧化葡萄糖和氢氧化镁的反应产生的氧被再利用于葡萄糖的氧化。
    • 27. 发明公开
    • 가압조건 하에서 포도당 산화효소를 이용한 글루콘산 칼슘의 제조방법
    • 在加压条件下使用葡萄糖氧化酶生产葡萄糖酸钙的方法
    • KR1019990061564A
    • 1999-07-26
    • KR1019970081837
    • 1997-12-31
    • 주식회사 신동방씨피
    • 성연섭지호순최길영도무회
    • C12P7/58
    • 본 발명은 가압 조건 하에서 포도당 산화 효소와 카탈라제를 이용하여 포도당에서 글루콘산 칼슘을 제조하는 방법에 관한 것이다.
      종래의 발효법에 의한 글루콘산 칼슘의 제조 방법은 포도당 글루콘산 나트륨 글루콘산 글루콘산 칼슘순으로 제조되는 바, 제조 공정이 복잡하고 제조 비용의 상승을 초래하였으며, 원료의 전처리, 발효균주의 보존, 복잡한 정제과정 및 폐수 및 폐기물 등이 문제되었다.
      이러한 단점을 극복하고자 발명된 본 발명은 미생물 대신 효소를 사용함으로서 목적 물질을 직접 생산하게 되므로 제조 공정이 축소될 뿐 만 아니라, 반응액을 연속적으로 재활용함으로서 폐수 발생을 줄일 수 있는 효과를 갖는다.
      본 발명에 의하면, 분말함수 포도당 혹은 정제된 액상 포도당을 10-40%의 농도로 반응액을 만든 후 고압산소로 1-2kgf/㎠정도의 가압을 한 조건하에서 포도당 산화 효소와 카탈라제를 첨가하여 포도당을 효소적으로 산화 시켜 글루콘산을 만들고 이와 동시에 중화제를 첨가한 후 신속하게 교반하여 글루콘산 칼슘을 제조한다.