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    • 12. 发明公开
    • 돼지 전염성위장염 (TGE) 코로나바이러스 및 유해 미생물 억제 활성을 갖는 신규 락토바실러스 프란타럼 Probio-38 및 이를 함유하는 생균활성제
    • 抑制致病微生物和磷酸化可变性胃肠炎(TGE)冠状病毒和PROBIOTICS COMPRIS的生长的耐受性优良的生物化学乳酸杆菌PROBIO-38
    • KR1020030063961A
    • 2003-07-31
    • KR1020020004329
    • 2002-01-24
    • 주식회사 프로바이오닉(주)엠디랩
    • 박용하이인선윤정훈김철중
    • C12N1/20
    • PURPOSE: An acid tolerant probiotic Lactobacillus plantarum Probio-38 that suppresses the growth of pathogenic microorganisms and porcine transmissible gastroenteritis(TGE) coronavirus and probiotics comprising the same microorganism are provided, thereby effectively preventing and treating diseases such as diarrhea of domestic animals caused by pathogenic microorganisms. CONSTITUTION: An acid tolerant probiotic Lactobacillus plantarum Probio-38(KCCM-10329) suppresses the growth of pathogenic microorganisms and porcine transmissible gastroenteritis(TGE) coronavirus. A pharmaceutical, veterinary or sitological composition having the probiotic activity contains the acid tolerant probiotic Lactobacillus plantarum Probio-38(KCCM-10329) and acceptable resins, wherein the composition optionally contains Lactobacillus reuterii Probio-16(KCCM-10214), Lactobacillus salivarius Probio-37(KCCM 10328) or a mixture thereof; and it is useful for the prevention or treatment of diarrhea of mammals when orally administered to the mammals. Probiotics for foods, medicines, animal medicines or fodder contain the acid tolerant probiotic Lactobacillus plantarum Probio-38(KCCM-10329) or the pharmaceutical, veterinary or sitological composition containing the acid tolerant probiotic Lactobacillus plantarum Probio-38(KCCM-10329).
    • 目的:提供一种耐酸益生菌植物乳杆菌Probio-38,其抑制致病微生物和猪传染性胃肠炎(TGE)冠状病毒和包含相同微生物的益生菌的生长,从而有效预防和治疗由病原体引起的家畜腹泻等疾病 微生物。 构成:耐酸益生菌植物乳杆菌Probio-38(KCCM-10329)抑制病原微生物和猪传染性胃肠炎(TGE)冠状病毒的生长。 具有益生菌活性的药物,兽医或静脉组合物含有耐酸益生菌植物乳杆菌Probio-38(KCCM-10329)和可接受的树脂,其中组合物任选地含有赖氏乳杆菌Probio-16(KCCM-10214),唾液乳杆菌Probio- 37(KCCM 10328)或其混合物; 对哺乳动物口服给药时,可以预防或治疗哺乳动物的腹泻。 食品,药品,动物药物或饲料的益生菌含有耐酸益生菌植物乳杆菌Probio-38(KCCM-10329)或含有耐酸益生菌植物乳杆菌Probio-38(KCCM-10329)的药物,兽医或静脉组合物。
    • 13. 发明公开
    • 돼지 전염성위장염 (TGE) 코로나바이러스 및 유해 미생물 억제 활성을 갖는 신규 락토바실러스 살리바리우스 Probio-37 및 이를 함유하는 생균활성제
    • 可抑制致病性乳酸杆菌沙门氏菌PROBIO-37,抑制病原微生物和磷酸盐可转移胃肠炎(TGE)的发生和癌胚抗原
    • KR1020030063960A
    • 2003-07-31
    • KR1020020004328
    • 2002-01-24
    • 주식회사 프로바이오닉(주)엠디랩
    • 박용하이인선윤정훈김철중
    • C12N1/20
    • PURPOSE: An acid tolerant probiotic Lactobacillus salivarius Probio-37 that can suppresses the growth of pathogenic microorganisms and porcine transmissible gastroenteritis(TGE) coronavirus and probiotics comprising the same are provided, thereby effectively improving the body weight and meat quality of domestic animals. CONSTITUTION: An acid tolerant probiotic Lactobacillus salivarius Probio-37(KCCM-10328) suppresses the growth of pathogenic microorganisms and porcine transmissible gastroenteritis(TGE) coronavirus. A pharmaceutical, veterinary or sitological composition having the probiotic activity contains the acid tolerant probiotic Lactobacillus salivarius Probio-37(KCCM-10328) and acceptable resins, wherein the composition optionally contains Lactobacillus reuterii Probio-16(KCCM-10214), Lactobacillus plantarum Probio-38(KCCM 10329) or a mixture thereof; and the composition is useful for the prevention or treatment of diarrhea of mammals when it is orally administered to the mammals. Probiotics for foods, medicines, animal medicines or fodder contain the acid tolerant probiotic Lactobacillus salivarius Probio-37(KCCM-10328) or the pharmaceutical, veterinary or sitological composition containing the acid tolerant probiotic Lactobacillus salivarius Probio-37(KCCM-10328).
    • 目的:提供一种耐酸益生菌唾液乳杆菌Probio-37,可以抑制致病微生物和猪传染性胃肠炎(TGE)冠状病毒和包含其的益生菌的生长,从而有效提高家畜的体重和肉质。 构成:耐酸益生菌唾液乳杆菌Probio-37(KCCM-10328)抑制病原微生物和猪传染性胃肠炎(TGE)冠状病毒的生长。 具有益生菌活性的药物,兽医或静态组​​合物含有耐酸益生菌唾液乳杆菌Probio-37(KCCM-10328)和可接受的树脂,其中组合物任选地含有赖氏乳杆菌Probio-16(KCCM-10214),植物乳杆菌Probio- 38(KCCM 10329)或其混合物; 并且该组合物可用于预防或治疗哺乳动物在口服给予哺乳动物时的腹泻。 食品,药品,动物药品或饲料的益生菌含有耐酸益生菌唾液乳杆菌Probio-37(KCCM-10328)或含有耐酸益生菌唾液乳杆菌Probio-37(KCCM-10328)的药物,兽医或静脉药物组合物。
    • 14. 发明授权
    • 인플루엔자 바이러스 항원의 세포표면 발현벡터 및 이에 의해 형질전환된 미생물
    • 用于流感病毒抗原和转化的微生物的细胞表面表达载体
    • KR101525180B1
    • 2015-06-04
    • KR1020140134245
    • 2014-10-06
    • 주식회사 바이오리더스충남대학교산학협력단
    • 홍승표노종복김철중이종수
    • C07K19/00C07K14/28C07K14/11C12N15/62A61K39/42A61P31/16
    • Y02A50/472
    • 본발명은인플루엔자바이러스의특이항원단백질인매트릭스 2(M2) 및헤마글루티닌(HA2)을미생물의표면에발현시키는벡터및 이에의해형질전환된미생물에관한것으로, 더욱자세하게는폴리감마글루탐산합성효소복합체유전자(pgsA), 콜레라독소 A1 서브유닛(CTA1), 인플루엔자바이러스의특이항원단백질인매트릭스 2(M2) 및헤마글루티닌(HA2)을코딩하는유전자를포함하는표면발현벡터, 상기벡터로형질전환된재조합미생물및 상기재조합미생물로부터수득한항원을유효성분으로함유하는인플루엔자바이러스감염의예방용백신에관한것이다. 본본 발명에따른콜레라독소 A1 서브유닛과결합된매트릭스 2 및헤마글루티닌(CTA1-sM2HA2) 항원단백질이표면발현된미생물은다양한 A형인플루엔자바이러스서브타입에우수한면역반응을유발하며, 상기인플루엔자바이러스의특이항원단백질인매트릭스 2 및헤마글루티닌을표면발현하는미생물은경구또는비강에직접적용할수 있는점막백신으로유용하다.
    • 本发明涉及用于在微生物的表面上表达流感病毒的特异性抗原蛋白质的基质2(M2)和血凝素(HA2)的载体,以及由其转化的微生物。 更具体地,本发明涉及包含用于编码聚-γ-谷氨酸合成酶复合物基因(pgsA),霍乱毒素A1亚基(CTA1)和基质2(M2)和血凝素(pagA)的基因的表面表达载体 HA2),流感病毒的特异性抗原蛋白,通过载体转化的重组微生物,以及含有由重组微生物获得的抗原作为活性成分的流感病毒感染疫苗。 根据本发明,在表面上表达与霍乱毒素A1亚基偶联的基质2(M2)和血凝素(CTA1-sM2HA2)抗原蛋白的微生物对各种A型流感引起优异的免疫反应 病毒亚型。 此外,用于在其表面上表达流感病毒的特异性抗原蛋白的基质2和血凝素表达的微生物可用于能够直接施用于口腔或鼻腔的粘膜疫苗。
    • 16. 发明公开
    • 신규 항시적 고발현 프로모터 및 그 용도
    • 新宪法强力促进者及其使用
    • KR1020080086161A
    • 2008-09-25
    • KR1020070027947
    • 2007-03-22
    • 주식회사 바이오리더스한국생명공학연구원
    • 성문희김철중홍승표부하령이일한김지연김광
    • C12N9/88C12N15/09C12N15/10
    • C12N15/746C12N9/1276
    • A novel constitutive strong promoter is provided to express effectively a target protein on the surface of a microorganism transformed by an expression vector containing the promoter, so that the transformed microorganism is useful as a vaccine carrier. An aldolase promoter isolated from Lactobacillus casei has the nucleotide sequence of SEQ ID NO:1. A microorganism surface expression vector contains the aldolase promoter, polygammaglutamic acid synthetase complex gene selected from pgsA, pgsB and pgsC and a gene encoding a target protein. A method for producing a microorganism vaccine comprises the steps of: (a) transforming a microorganism with the microorganism surface expression vector and culturing the transformed microorganism to express an antigen on the surface of transformed microorganism; and (b) recovering the antigen surface-expressed microorganism, wherein the microorganism is a lactic acid bacterium.
    • 提供了一种新颖的组成型强启动子,以有效地表达由含有启动子的表达载体转化的微生物的表面上的靶蛋白,使得转化的微生物可用作疫苗载体。 从干酪乳杆菌分离的醛缩酶启动子具有SEQ ID NO:1的核苷酸序列。 微生物表面表达载体含有选自pgsA,pgsB和pgsC的醛缩酶启动子,聚氨基amic氨酸合成酶复合物基因和编码靶蛋白的基因。 微生物疫苗的制造方法包括以下步骤:(a)用微生物表达载体转化微生物,培养转化的微生物以在转化的微生物的表面上表达抗原; 和(b)回收抗原表面表达微生物,其中微生物是乳酸菌。
    • 19. 发明授权
    • 신규 알카리성 리파제, 이를 생산하는 신규 바실러스 균주 및 리파제 대량생산 방법
    • 신규알카리성리파제,이를생산하산신규바실러스균주및리파제대량생산방
    • KR100463350B1
    • 2004-12-29
    • KR1020010061667
    • 2001-10-06
    • 한국생명공학연구원주식회사 바이오리더스
    • 성문희오태광김형권최윤호김광석김영선김철중송재준김광배진우신광순부하령이상철
    • C12N9/14
    • PURPOSE: Provided are a novel alkaline lipase, a novel bacillus sp. strain producing the same, and a method of producing the lipase massively. The non-toxicity bacillus sp. strain is capable of being used in food industry, and the lipase is used for quantitative determination of lipid content. CONSTITUTION: The novel alkaline lipase shows thermostability at 40 deg.C and has an optimum temperature of activity at 35 deg.C. Further, it has no changes with calcium concentrations, and has an optimum activity at pH 8.5. Its amino acid sequence is represented by the SEQ ID NO:1. The novel bacillus sp. strain, bacillus pumilus B26 strain(KTCC 10081BP) produces the lipase. The lipase is mass-produced by transforming bacillus subtilis DB104 with a recombinant vector pMB26 then culturing the transformant. The transformant strain is bacillus subtilis DB104/pMB26(KCTC 10082BP).
    • 目的:提供了一种新型碱性脂肪酶,一种新型的芽孢杆菌。 产生它的菌株,以及大量生产脂肪酶的方法。 无毒芽孢杆菌 菌株能够在食品工业中使用,并且脂肪酶用于定量测定脂质含量。 构成:新型碱性脂肪酶在40摄氏度显示热稳定性,在35摄氏度时具有最佳活性温度。 此外,钙浓度没有变化,并且在pH 8.5时具有最佳活性。 其氨基酸序列由SEQ ID NO:1表示。 新型芽孢杆菌 菌株,短小芽孢杆菌B26菌株(KTCC10081BP)产生脂肪酶。 通过用重组载体pMB26转化枯草芽孢杆菌DB104然后培养转化体来大量生产脂肪酶。 转化株是枯草芽孢杆菌DB104 / pMB26(KCTC10082BP)。