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    • 13. 发明公开
    • 디노피시스 아쿠미나타의 대량배양 및 이로부터 펙테노톡신-2의 분리 방법
    • 大肠杆菌大量培养的方法和磷酸二辛酯-2的分离
    • KR1020100035055A
    • 2010-04-02
    • KR1020080094357
    • 2008-09-25
    • 군산대학교산학협력단
    • 이원호노정래김형섭
    • C12N1/12C12N1/20C12N1/00
    • C12N1/10C07D493/22C12P17/181
    • PURPOSE: A method for massively culturing Dinophysis acuminate is provided to massively culture Dinophysis acuminate and use as a standard toxin when analyzing toxin component. CONSTITUTION: A method for massively culturing Dinophysis acuminate comprises: a step of inoculating Myrionecta rubra and teleaulax in a density ratio of 1:10-1:15; a step of sub-culturing Myrionecta rubra at 10-30°C under serial light; a step of inoculating cultured Myrionecta rubra in a water bath to which air is supplied; a step of stirring Myrionecta rubra and seawater medium and culturing Myrionecta rubra massively; a step of inoculating Myrionecta rubra and Dinophysis acuminate in an initial density of 1:10-1:30; a step of sub-culturing the Dinophysis acuminate at 10-30°C; and a step of inoculating Dinophysis acuminate in a water bath. The seawater is f/2-Si medium.
    • 目的:大规模培养D uring A A A A A A A A to to。。。。。。。。。。。。。。。。。。。。。。。。。 构成:用于大规模培养脱色剂的方法包括:以1:10-1:15的密度比接种Myrionecta rubra和teleaulax的步骤; 在10-30°C的串联光下培养Myrionecta rubra的步骤; 将培养的Myrionecta rubra接种在供应空气的水浴中的步骤; 搅拌Myrionecta rubra和海水培养基,大量培养Myrionecta rubra的步骤; 以1:10-1:30的初始密度接种Myrionecta rubra和Dinophiva acuminate的步骤; 在10-30℃下分解培养Dinophtic acuminate的步骤; 以及在水浴中接种褪黑激素的步骤。 海水是f / 2-Si介质。