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    • 91. 发明公开
    • 중간엽 줄기세포로 췌장염을 치료하는 방법
    • 用MESENCHYMAL STEM细胞治疗胰腺癌的方法
    • KR1020130106432A
    • 2013-09-27
    • KR1020137020033
    • 2011-12-30
    • 인하대학교 산학협력단
    • 송순욱홍순선정경희이돈행
    • A61K35/28A61K35/14A61K35/44A61P1/18
    • A61K35/12A61K35/14A61K35/28A61K35/35A61K35/51
    • PURPOSE: A method for treating pancreatitis using mesenchymal stem cells is provided to treat and alleviate pancreatitis by administering stem cells or precursor cells to a patient with pancreatitis. CONSTITUTION: A method for treating pancreatitis, reducing pancreatic edema or relative weight of pancreas, increasing acinar cell population, or preventing necrosis of acinar cells in a patient with pancreatitis comprises the steps of: collecting a bone marrow, peripheral blood, cord blood, or adipose sample or a biological sample from cytokine-activated peripheral blood; precipitating the sample in a container; carrying a supernatant into other container through two or more rounds; isolating cells from the supernatants; and administering the isolated cells to a patient with pancreatitis. The sample is collected by centrifugation under 1,000 rpm. [Reference numerals] (AA) Bone marrow; (BB) Transmit supernatant; (CC,DD,EE) Isolate sole colony
    • 目的:提供使用间充质干细胞治疗胰腺炎的方法,通过向患有胰腺炎的患者施用干细胞或前体细胞来治疗和缓解胰腺炎。 构成:一种治疗胰腺炎,减少胰腺水肿或胰腺相对重量,增加腺泡细胞群体或预防胰腺炎患者腺泡细胞坏死的方法,包括以下步骤:收集骨髓,外周血,脐带血或 脂肪样品或来自细胞因子激活的外周血的生物样品; 将样品沉淀在容器中; 通过两次或更多次将上清液携带到其他容器中; 从上清液中分离细胞; 并将分离的细胞施用于患有胰腺炎的患者。 通过在1,000rpm下离心收集样品。 (附图标记)(AA)骨髓; (BB)转染上清液; (CC,DD,EE)隔离单个菌落
    • 97. 发明公开
    • 엔도스타틴 및 카복실에스터라제가 동시 과발현된 간엽줄기세포 및 이를 이용하여 신경교종을 예방, 치료 또는 재발 억제하는 방법
    • 间歇性干细胞同时超表达内皮素和羧酰基转移酶,含有其的药物组合物和使用该方法预防,治疗和重新抑制GLIOMA的方法
    • KR1020120114468A
    • 2012-10-17
    • KR1020110031979
    • 2011-04-07
    • 서울대학교산학협력단
    • 최윤재김형기윤금룡김훈김준겸
    • C12N5/0775A61K35/28
    • C12N5/0668A61K35/28
    • PURPOSE: A mesenchymal stem cell in which endostatin and carboxyl esterase are concurrently over expressed and preventing, treating or recurrence suppression method of glioma by using the same are provided to drastically prevent carcinogenesis and recurrence. CONSTITUTION: In a mesenchymal stem cell, endostatin and carboxyl esterase are concurrently over expressed. A preventing, treating or recurrence suppression method of glioma includes a step of injecting the mesenchymal stem cell to all mammals except for human. A manufacturing method of human amniotic fluid-derived mesenchymal stem cell(AF-MSC) comprises the following steps: establishing positive number of primary cell cultivation in a minimum essential medium in which fetal bovine serum(ES-FBS) having 15% of embryonic stem cell, 2mM L-glutamine and 1% of penicillin streptomycin are contained, and 18% Chang culture medium B and 2% Chang culture medium C are supplemented; collecting cells by trypsinization for selective cultivation of AF-MSC; plating in DMEM low dextrose broth supplemented with 10% of FBS, 1% of penicillin streptomycin, 2mM L-glutamine and 4ng/mL basic fibroblast growth factor (bFGF: R&D); and obtaining morphologic interspecies population of the AF-MSC after second round of the subculture.
    • 目的:提供内皮抑素和羧基酯酶同时过表达的间充质干细胞,通过使用胶质瘤预防,治疗或复发抑制方法,大幅度预防癌变和复发。 构成:在间充质干细胞中,内皮抑素和羧基酯酶同时过度表达。 胶质瘤的预防,治疗或复发抑制方法包括将间充质干细胞注射到除人之外的所有哺乳动物的步骤。 人类羊水间充质干细胞(AF-MSC)的制备方法包括以下步骤:在具有15%的胚胎干细胞的胎牛血清(ES-FBS)的最小必需培养基中建立阳性数量的原代细胞培养 细胞,2mM L-谷氨酰胺和1%青霉素链霉素,并补充18%Chang培养基B和2%Chang培养基C; 通过胰蛋白酶消化来收集细胞以选择性培养AF-MSC; 在补充有10%FBS,1%青霉素链霉素,2mM L-谷氨酰胺和4ng / mL碱性成纤维细胞生长因子(bFGF:R&D)的DMEM低葡萄糖肉汤中电镀; 并在第二轮传代培养后获得AF-MSC的形态种间群体。
    • 98. 发明公开
    • 지방유래 중간엽 줄기세포를 연골세포로 분화시키는 방법
    • ADIPOSE组织衍生的MESENCHYMAL STEM细胞的CHENDROGENESIS差异
    • KR1020120111790A
    • 2012-10-11
    • KR1020110029489
    • 2011-03-31
    • 동국대학교 산학협력단
    • 임군일김혜정
    • C12N5/077A61K35/28A61F2/30C12N15/87
    • C12N5/0655A61K35/28C12N2501/60A61F2/30C12N15/87C12N2506/1346
    • PURPOSE: A method for differentiating adipose-derived mesenchymal stem cells into chondrocytes is provided to reduce immunorejection during transplantation. CONSTITUTION: A method for differentiating adipose-derived mesenchymal stem cells into chondrocytes comprises: a step of introducing SOX-5, SOX-6 and SOX-9 genes to the stem cells by electroporation at 1300-1500 voltage 1-3 times; and a step of culturing the stem cells. A culture medium contains insulin, dexamethasone, ascorbate, 2-phosphate, L-proline, or sodium pyruvate. A composition for treating cartilage injury contains chondrocytes. A method for manufacturing artificial joint comprises a step of culturing the differentiated chondrocytes in a scaffold. [Reference numerals] (AA) Comparative embodiment 1; (BB) Comparative embodiment 2; (CC) Comparative embodiment 3; (DD) Comparative embodiment 4; (EE) Embodiment; (FF) Comparative embodiment 5; (GG) Type II collagen; (HH) Type I collagen; (II) Type X collagen
    • 目的:提供一种将脂肪来源的间充质干细胞分化成软骨细胞的方法,以减少移植期间的免疫注射。 构成:将脂肪来源的间充质干细胞分化为软骨细胞的方法包括:通过1300-1500次电压1-3次电穿孔将SOX-5,SOX-6和SOX-9基因导入干细胞的步骤; 以及培养干细胞的步骤。 培养基含有胰岛素,地塞米松,抗坏血酸,2-磷酸,L-脯氨酸或丙酮酸钠。 用于治疗软骨损伤的组合物含有软骨细胞。 制造人造关节的方法包括在支架中培养分化软骨细胞的步骤。 (附图标记)(AA)比较实施例1; (BB)比较实施例2; (CC)比较实施例3; (DD)比较实施例4; (EE)实施例; (FF)比较实施例5; (GG)II型胶原; (HH)I型胶原蛋白; (II)X型胶原蛋白