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    • 8. 发明专利
    • Method for identifying factor for differentiating definitive endoderm
    • 用于鉴别定义性内切酶的因子的方法
    • JP2014147392A
    • 2014-08-21
    • JP2014081238
    • 2014-04-10
    • Viacyte Incヴィアサイト,インコーポレイテッド
    • D AMOUR KEVIN ALLENAGULNICK ALAN DELIAZER SUSANBAETGE EMMANUEL
    • C12Q1/02C07K14/71C12N5/00C12N5/071C12N5/073C12N5/0735
    • C12N5/0018A61K35/12C12N5/0603C12N5/0606C12N5/0676C12N5/0679C12N15/1086C12N2500/90C12N2501/115C12N2501/119C12N2501/155C12N2501/16C12N2501/385C12N2501/415C12N2502/02C12N2502/13C12N2503/00C12N2506/02C12N2510/00C12Q1/6881C12Q2600/158
    • PROBLEM TO BE SOLVED: To provide an efficient directed differentiation method of hESC (human Embryonic Stem Cell or human Embryonic Germ Cell) as a starting material for generating a useful cell in a cell therapy application.SOLUTION: There is disclosed a method for identifying a differentiation factor which can promote the differentiation of human definitive endoderm cells in a cell population comprising human cells, and the method comprising the step of obtaining a cell population comprising human definitive endoderm cells, the step of supplying a candidate differentiation factor to the cell population, the step of measuring expression of a marker in the cell population at the first time point, the step of measuring expression of the same marker in the cell population at the second time point, and the step of determining whether expression of the marker in the cell population at the second time point is altered as compared to the expression of the marker in the cell population at the first time point, wherein the second time point is subsequent to supplying the cell population with the candidate differentiation factor, and the alteration in expression of the marker in the cell population indicates that the candidate differentiation factor can promote the differentiation of the human definitive endoderm cells.
    • 要解决的问题:提供hESC(人胚胎干细胞或人胚胎细胞)的有效的定向分化方法作为在细胞治疗应用中产生有用细胞的起始材料。解决方案:公开了一种用于鉴定 可促进包含人细胞的细胞群体中的人定形内胚层细胞分化的分化因子,以及包括获得包含人定形内胚层细胞的细胞群的步骤的方法,向细胞群体提供候选分化因子的步骤, 在第一时间点测量细胞群体中的标记物的表达的步骤,在第二时间点测量细胞群体中相同标记物的表达的步骤以及确定细胞中标志物的表达的步骤 与第二时间点的细胞群体中的标记物的表达相比,第二时间点的群体被改变 第一时间点,其中第二时间点在向细胞群体提供候选分化因子之后,并且标志物在细胞群体中的表达的改变表明候选分化因子可以促进人类确定性内胚层细胞的分化 。