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    • 3. 发明专利
    • Apparatus and method for filter cleaning by ultrasound, backwashing and filter movement during the filtration of biological samples
    • 在生物样品过滤过程中通过超声波清洗和过滤器运动进行过滤清洗的装置和方法
    • JP2013165724A
    • 2013-08-29
    • JP2013078465
    • 2013-04-04
    • Smith & Nephew Plcスミス アンド ネフュー ピーエルシーSmith & Nephew Public Limited Company
    • CURRAN STEPHEN
    • C12M1/26B01D33/15B01D35/20C12M1/12
    • C02F1/34B01D29/62B01L3/50255B01L2400/0436B01L2400/0487C02F2209/005
    • PROBLEM TO BE SOLVED: To provide apparatus and methods for separating a solid fraction from a fluid sample.SOLUTION: The present application is directed to the separation of a solid fraction from a fluid sample particularly a therapeutic cellular fraction from a biological sample such as a bone marrow sample by a porous filter (2). The porous filter (2) separates a filtration unit (1) into an upper pre-filtration chamber (3) into which a fluid sample (4) requiring cell separation is introduced and a lower post-filtration chamber (5) into which a fluid (6) capable of transmitting an acoustic standing wave is introduced. An acoustic element (8) is coupled to a substrate (7) which is located within and at the bottom of the lower chamber (5) and which resonates in response to the acoustic generating element (8) and generates a standing wave through the two fluid phases and the filter to agitate the sample (4). Simultaneously, a cyclic process of vacuum draw (9) causes movement of the sample (4) downwards through the filter (2). Vacuum pressure, fluid flow rate and frequency of vibration are controlled from a remote unit housing appropriate pumps and valves.
    • 要解决的问题:提供用于从流体样品中分离固体部分的装置和方法。解决方案:本申请涉及将固体部分与流体样品分离,特别是来自生物样品的治疗性细胞部分,例如 骨髓样品通过多孔过滤器(2)。 多孔过滤器(2)将过滤单元(1)分离成上部预过滤室(3),其中引入需要细胞分离的流体样品(4)和下部后过滤室(5),其中流体 (6)能够传输声驻波。 声学元件(8)耦合到位于下部腔室(5)的内部并在其底部并且响应于声学发生元件(8)谐振并且通过两个声学元件产生驻波的衬底(7) 流体相和过滤器搅拌样品(4)。 同时,抽真空(9)的循环过程使得样品(4)向下移动通过过滤器(2)。 真空压力,流体流速和振动频率由远程单元控制适当的泵和阀门。
    • 4. 发明专利
    • Biological substance analyzing method and biological substance analyzing cell, chip and device used therein
    • 生物物质分析方法和生物物质分析细胞,芯片和使用的装置
    • JP2011174857A
    • 2011-09-08
    • JP2010040088
    • 2010-02-25
    • Fujifilm Corp富士フイルム株式会社
    • HORII KAZUYOSHINAKAYAMA RYUICHIHIRABAYASHI YASUTOSHI
    • G01N33/543G01N21/78G01N33/483
    • G01N33/5438B01L3/50273B01L3/502761B01L2300/0809B01L2300/0819B01L2300/0877B01L2300/168B01L2400/0436
    • PROBLEM TO BE SOLVED: To rapidly detect a substance to be inspected in a sensor part with high efficiency in the analysis of a biological substance and to increase the speed and sensitivity of the analysis of the biological substance. SOLUTION: In the analysis of the biological substance for detecting the substance A to be inspected, a biological substance analyzing cell 10, which is equipped with a reaction tank 12 having a sample supply space 12a, the acoustic matching layer 14 provided to the predetermined region of the inner wall of the reaction tank 12 and the sensor part 15 provided in the reaction tank 12, is used to emit ultrasonic waves between the interface 14a of the acoustic matching layer 14 and the sample supply space 12a and the inner wall of the reaction tank 12 opposed to the acoustic matching layer 14 so as to produce a standing wave U and the substance A to be inspected is concentrated by the capturing force of the standing wave U to detect the concentrated substance A to be inspected by the sensor part 15. COPYRIGHT: (C)2011,JPO&INPIT
    • 要解决的问题:为了在生物物质分析中以高效率快速检测传感器部件中的待检物质,并提高生物物质分析的速度和灵敏度。 解决方案:在用于检测待检查物质A的生物物质的分析中,配备有具有样品供应空间12a的反应槽12的生物物质分析池10,提供给声学匹配层14 反应槽12的内壁的预定区域和设置在反应槽12中的传感器部分15用于在声匹配层14的界面14a与样品供应空间12a和内壁之间发射超声波 与声匹配层14相对的反应槽12,以产生驻波U,待检查物质A被驻波U的捕获力集中,以检测由传感器检查的浓缩物质A 第15部分。版权所有(C)2011,JPO&INPIT
    • 10. 发明专利
    • Dynamically controllable biological/chemical detector having nano-structure surface
    • 具有纳米结构表面的动态可控生物/化学检测器
    • JP2005274573A
    • 2005-10-06
    • JP2005083221
    • 2005-03-23
    • Lucent Technol Incルーセント テクノロジーズ インコーポレーテッド
    • ARNEY SUSANNEKROUPENKINE TIMOFEI NLYONS ALAN MMARY LOUISE MANDICHSCHABEL MICHAEL JTAYLOR JOSEPH ASHLEY
    • G01N27/447B01L3/00B82B1/00G01N37/00
    • B01L3/5088B01L3/508B01L2300/089B01L2300/161B01L2400/0406B01L2400/0421B01L2400/0427B01L2400/0436
    • PROBLEM TO BE SOLVED: To provide an integrated and dynamically controllable biological/chemical detector capable of operating a liquid such as a droplet of a reagent crossing a nano-structure surface independently from a minute passage. SOLUTION: In a first embodiment, a fluid flow is passed through the detector, whereby particles entirely or partially containing a typical chemical compound or biological species are collected on the tip of the nano-structure in the detector. A droplet is moved across the tip of the nano-structure, whereby particles are absorbed in a liquid. The droplet is penetrated into the nano-structure in a desired position, whereby the chemical compound or biological species in the droplet is brought into contact with, for example, a reagent. In another embodiment, the fluid flow is passed through the nano-structure surface of the detector so that the chemical compound and/or the biological species are accumulated between nano-columns of a desired pixel. The droplet is moved to the desired pixel across the surface, penetrated into the nano-structure of the pixel, and thus brought into contact with the reagent. COPYRIGHT: (C)2006,JPO&NCIPI
    • 要解决的问题:提供一种集成且动态可控的生物/化学检测器,其能够独立于微通道操作诸如与纳米结构表面交叉的试剂的液滴的液体。 解决方案:在第一实施例中,使流体流动通过检测器,由此将完全或部分含有典型化学化合物或生物物质的颗粒收集在检测器中的纳米结构的尖端上。 微滴移动穿过纳米结构的尖端,由此颗粒被吸收在液体中。 液滴在期望的位置被渗透到纳米结构中,由此液滴中的化合物或生物物质与例如试剂接触。 在另一个实施方案中,流体流动通过检测器的纳米结构表面,使得化学化合物和/或生物物质在所需像素的纳米柱之间累积。 液滴通过表面移动到期望的像素,穿透到像素的纳米结构中,并因此与试剂接触。 版权所有(C)2006,JPO&NCIPI