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    • 2. 发明专利
    • Method for producing mesenchymal cell, method for producing tooth and mesenchymal cell for formation of tooth
    • 用于生产细胞的方法,用于生产牙齿和用于形成牙齿的细胞的方法
    • JP2008200033A
    • 2008-09-04
    • JP2008008772
    • 2008-01-18
    • Tokyo Univ Of Science学校法人東京理科大学
    • TSUJI TAKASHIMORITA RITSUKO
    • A61K35/12A61L27/00A61P1/02C12N5/077C12N15/09
    • C12N5/0654A61K6/02A61K35/12C12N2500/30C12N2500/62C12N2501/385C12N2506/02
    • PROBLEM TO BE SOLVED: To provide a mesenchymal cell for formation of tooth capable of efficiently obtaining tooth retaining characteristic cell configuration and efficiently obtain the tooth in which characteristic cell configuration is retained. SOLUTION: A totipotent stem cell, e.g. embryonic carcinoma cell is cultured in the presence of a differentiation-inducing agent such as dimethyl sulfoxide, and the CD44-positive and CD29-positive cell or the CD44-positive and CD106-positive cell is selected from the differentiation-induced cell population as a tooth-forming mesenchymal cell. A first cell population comprising substantially only either one of a mesenchymal cell and an epithelial cell and a second cell population comprising substantially only the other are placed in the inside of a carrier which can allow the cells to be in contact with each other in a closely adhered state without mixing them and the cell populations are cultured, wherein the mesenchymal cell comprises the tooth-forming mesenchymal cell. As a result, the tooth having characteristic cell configuration is obtained. COPYRIGHT: (C)2008,JPO&INPIT
    • 要解决的问题:提供一种用于形成牙齿的间充质细胞,其能够有效地获得牙齿保持特性细胞构型,并有效地获得保留特征细胞构型的牙齿。 解决方案:全能干细胞,例如 在分化诱导剂如二甲基亚砜存在下培养胚胎癌细胞,CD44阳性和CD29阳性细胞或CD44阳性和CD106阳性细胞选自分化诱导的细胞群,作为 造齿间充质细胞。 基本上仅包含间充质细胞和上皮细胞中的任一种的第一细胞群和基本上仅包含另一种的第二细胞群被放置在载体的内部,所述载体可以允许细胞彼此紧密接触 在不混合的情况下培养细胞群,培养细胞群,其中间质细胞包含形成牙齿的间充质细胞。 结果,获得具有特征单元结构的齿。 版权所有(C)2008,JPO&INPIT