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1. 发明专利

JPS6313675B2 失效
公开(公告)号：JPS6313675B2
公开(公告)日：1988-03-26
申请号：JP7545880
申请日：1980-06-06
申请人： TORAY INDUSTRIES
发明人： KUBOTA HIDEMASA , IIZUKA MASAHIKO , KIZAKI TAKAFUMI
IPC分类号： C12N5/02 , C12N5/00 , C12N5/07 , C12N5/071 , C12P21/00 , C12R1/91

2. 发明专利

JPH07270415A ENZYME IMMUNOASSAY 失效
公开(公告)号：JPH07270415A
公开(公告)日：1995-10-20
申请号：JP6390994
申请日：1994-03-31
申请人： TORAY INDUSTRIES
发明人： YONEZAWA SHUHEI , KIZAKI TAKAFUMI , ICHIKURA SHIGERU
IPC分类号： G01N33/535
摘要： PURPOSE:To prevent the sensitivity from fluctuating and carry out a stable and highly sensitive assay by obtaining the optimum hydrogen peroxide concentration based on a response curve between the hydrogen peroxide concentration and the sensitivity in a substrate solution and applying the concentration. CONSTITUTION:An aiming concentration of an object substance to be assayed is set based on the reaction conditions set for every kit and signal detection method and under the conditions, the hydrogen peroxide concentration in a substrate solution is changed, and a response curve between the obtained signal intensities and the hydrogen peroxide concentrations is drawn. Then, the hydrogen peroxide concentration at which the aiming optimum sensitivity value is obtained is calculated from the response curve. The concentration range is 0.002-0.5% (w/v), preferably 0.01-0.2% (w/v). The aiming sensitivity value is commonly set to be 0.8-2.5 as absorbance for the upper limit for the assay of the substance to be assayed in the case a where absorbance is employed. A labelled reference substance is mainly protein of such as antibodies, antigens, avidin, etc. A labelling method based on organic chemical bonding, an immunological labelling method, etc., are employed for the labelling method for a substance to be labelled by peroxidase. A sandwiching method using a peroxidase- labelled antibody is preferable for the measurement.

3. 发明专利

JPS6244176A METHOD FOR PASSAGE OF ANIMAL CELL 失效
公开(公告)号：JPS6244176A
公开(公告)日：1987-02-26
申请号：JP18183085
申请日：1985-08-21
申请人： TORAY INDUSTRIES
发明人： ICHIKURA SHIGERU , YAMAZAKI TORU , KIZAKI TAKAFUMI
IPC分类号： C12N5/02
摘要： PURPOSE:To aim improvement in cell passage from microcarrier to microcarrier, by using a specific buffer solution for washing a cell attached to a microcarrier and passaging a cultivated animal cell to the microcarrier. CONSTITUTION:A desired animal cell is cultivated according to a conventional microcarrier method to give a call attached to the a microcarrier. A microcarrier having a positively charged chemical residue or a carrier coated with modified collagen is generally used as the microcarreir. Any cell having anchor dependence may be used as the animal cell and a fibroblast derived from human newborn sheath, etc., may be cited. The cell attached to the microcarrier is washed with a buffer solution containing an amino acid vitamin medium, glucose and methyl cellulose before a passage operation is carried out.

4. 发明专利

JPS575690A CIRCULATION DEVICE FOR CULTURE MEDIUM IN CULTIVATION TANK 失效
公开(公告)号：JPS575690A
公开(公告)日：1982-01-12
申请号：JP7911980
申请日：1980-06-13
申请人： TORAY INDUSTRIES
发明人： KUBOTA HIDEMASA , KIZAKI TAKAFUMI
IPC分类号： C12M3/02 , C12N5/02
摘要： PURPOSE:A vertical cylinder in which baffle plates are built is furnished in the cultivation tank and the cylinder is connected with the pipe for taking out the culture mixture, whereby a predetermined amount of the culture mixture is circulated stably for a long time. CONSTITUTION:In the cultivation tank 1, the vertical cylinder 3 is set so that the opening turns downward and at least one of baffle plates 4 are built in vertically. The top end of the cylinder is connected with the pipe for taking out the culture mixture 5. The culture mixture is taken out by the pump 6 and the mixture taken out is subjected to necessary treatment with the device 7 and sent back through pipe 5' to the cultivation tank.

5. 发明专利

JPS6357037B2 失效
公开(公告)号：JPS6357037B2
公开(公告)日：1988-11-10
申请号：JP7911980
申请日：1980-06-13
申请人： TORAY INDUSTRIES
发明人： KUBOTA HIDEMASA , KIZAKI TAKAFUMI
IPC分类号： C12M3/02 , C12N5/02

6. 发明专利

JPS575689A UNIT FOR MICROCARRIER CULTIVATION 失效
公开(公告)号：JPS575689A
公开(公告)日：1982-01-12
申请号：JP7911780
申请日：1980-06-13
申请人： TORAY INDUSTRIES
发明人： KUBOTA HIDEMASA , KIZAKI TAKAFUMI
IPC分类号： C12M3/02
摘要： PURPOSE:A porous diaphragm is set at the lower part of the cultivation tank to conduct the exchange of culture medium and washing of the cells and microcarriers in a short time efficiently. CONSTITUTION:The porous diaphragm 8 is demountably set at the lower part of the cultivation tank 1 by means of, e.g., the flange 9 or the like. The stopping valve 11 and the draining nozzle 6 are provided on the bottom side of the diaphragm 8. The porous diaphragm permits liquids such as the culture medium to pass through but not the microcarriers and is required not to elute the components inhibiting the cell growth.

7. 发明专利

JPS572682A WASHING METHOD OF CELL 失效
公开(公告)号：JPS572682A
公开(公告)日：1982-01-08
申请号：JP7545880
申请日：1980-06-06
申请人： TORAY INDUSTRIES
发明人： KUBOTA HIDEMASA , IIZUKA MASAHIKO , KIZAKI TAKAFUMI
IPC分类号： C12N5/02 , C12N5/00 , C12N5/07 , C12N5/071 , C12P21/00 , C12R1/91
摘要： PURPOSE:To prevent the peeling of cells from a microcarrier surface in washing thereof, by washing the cells grown on the microcarrier surface with an aqueous solution of a salt containing a nonproteinic water-soluble high polymeric substance. CONSTITUTION:Cells grown on a microcarrier surface are washed with an aqueous solution of a salt adjusted to a suitable osmotic pressure. In the process, a nonproteinic water-soluble high polymeric substance, e.g. dextran, methyl cellulose or polyvinyl pyrrolidone, in an amount in the range of 0.0001-1wt%, preferably 0.001-0.1wt%, is added to the aqueous solution.

8. 发明专利

JPH07270414A HIGHLY SENSITIVE ENZYME IMMUNOASSAY 失效
公开(公告)号：JPH07270414A
公开(公告)日：1995-10-20
申请号：JP6270594
申请日：1994-03-31
申请人： TORAY INDUSTRIES
发明人： TAMAKI HIDEAKI , KIZAKI TAKAFUMI , ICHIKURA SHIGERU
IPC分类号： G01N33/535
摘要： PURPOSE:To detect a slight amount of antigen contained in blood by employing a peroxydase-labelled antibody separated and refined by using a hydroxyapatite column and having an aiming bonding ratio for an enzyme immunoassay EIA. CONSTITUTION:Uniform monochronal antibodies to carry out complete separation are preferably used as an antibody. Any substances commonly used as an antigen are used as an antigen to be made immune in order to obtain an antibody. As an enzyme for bonding with the antibody, a peroxydase is preferably used and glutaric dialdehyde, periodic acid method, etc., are employed for the bonding method. A general or high speed column may be employed for the hydroxyapatite column. A sodium phosphate buffer solution may be used as an eluent for sample elution. To obtain an antibody with 1-2 bonding ratio, absorbance (A280, A403) of the eluted solutions obtained by fractionation while monitoring with ultraviolet absorbance at 280nm is measured, the antibody concentration and HRD concentration are calculated, and the solutions whose bonding ratio is 1-2 are gathered.

9. 发明专利

JPS62100284A MULTIPLICATION CULTURE MEDIUM FOR CELL PRODUCING INTERFERON 失效
公开(公告)号：JPS62100284A
公开(公告)日：1987-05-09
申请号：JP23961985
申请日：1985-10-28
申请人： TORAY INDUSTRIES
发明人： WADA TAKAKO , ICHIKURA SHIGERU , KIZAKI TAKAFUMI , YAMAZAKI TORU
IPC分类号： C12N5/00 , C12N5/07 , C12N5/071 , C12P21/00
摘要： PURPOSE:To mass-produce interferon of high potency, by using a culture medium containing a specific concentration of glucose as a culture medium in a stage of multiplying animal cells. CONSTITUTION:The multiplication of animal cells is carried out in a culture medium having 1.2-1.8g/L glucose concentration in the charging composition of the culture medium to produce interferon using animal cells. An inducer, e.g. Sendai virus poly(I):poly(C), is added to the cells to give stimulation and release interferon into the culture medium. In the case of normal diploid cells, the animal cells are multiplied in a multiplication culture medium having 1.2-1.8g/L glucose concentration by a microcarrier method, etc., and then treated with a culture medium containing interferon and carboxymethyl cellulose in low concentrations to stimulate the cells with an interferon inducer. The stimulated cells are then transferred to a new culture medium to produce the interferon in the culture medium.

10. 发明专利

JPS6244177A METHOD FOR REPEATING USE OF MICROCARRIER 失效
公开(公告)号：JPS6244177A
公开(公告)日：1987-02-26
申请号：JP18183185
申请日：1985-08-21
申请人： TORAY INDUSTRIES
发明人： ICHIKURA SHIGERU , YAMAZAKI TORU , KIZAKI TAKAFUMI
IPC分类号： C12N5/02
摘要： PURPOSE:To make it possible to use an expensive microcarreir repeatedly in culture of an animal cell, by treating a microcarrier having been used in an animal cell culture with an alkali and then with a buffer solution and reusing it. CONSTITUTION:A microcarrier which has been used for animal cell culture and for production of a substance such as interferon is treated with 0.01-1 normal alkali and then with a buffer solution at 6-8pH. Then, the microcarrier is reused. A microcarrier having a positively charged chemical residue is generally used and very small particle of synthetic high polymer (e.g., very small particles of polystyrene, etc.,) very small inorganic particles (e.g., very small particles of glass), etc., may be used besides it. Any cell having anchor dependence may be used as the animal cell. For example, this process is applied to a cell such as a fibroblast derived from newborn sheath, fibroblast L derived from mouse, etc.

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