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1. 发明专利

JPS5420191A PURIFICATION OF UROKINASE 失效
公开(公告)号：JPS5420191A
公开(公告)日：1979-02-15
申请号：JP8367977
申请日：1977-07-12
申请人： TANABE SEIYAKU CO
发明人： SENHATA ICHIROU , KAKIMOTO TOSHIO , SHIBATANI TAKEYA , NISHIMURA NORIYUKI
IPC分类号： C12N9/72
摘要： PURPOSE:To recover highly purified urokinase from a crude urokinase solution in high yield, using a gel filtration material comprising polyacrylamide gel and agarose gel.

2. 发明专利

JPS5417180A PURIFICATION OF UROKINASE 失效
公开(公告)号：JPS5417180A
公开(公告)日：1979-02-08
申请号：JP8231077
申请日：1977-07-08
申请人： TANABE SEIYAKU CO
发明人： SENHATA ICHIROU , KAKIMOTO TOSHIO , SHIBATANI TAKEYA , NISHIMURA NORIYUKI
IPC分类号： C12N9/72 , B01D39/04
摘要： PURPOSE:To purify crude urokinase by the gel filtration using cross-linked allyl dextrane gel.

3. 发明专利

JPH0242992A REACTION USING BIOCATALYST AND DEVICE FOR SAID REACTION 失效
公开(公告)号：JPH0242992A
公开(公告)日：1990-02-13
申请号：JP19401088
申请日：1988-08-03
申请人： TANABE SEIYAKU CO
发明人： TOSA TETSUYA , SENUMA MASARU , SAKATA NOBUYUKI , NISHIMURA NORIYUKI , FURUI MASAKATSU
IPC分类号： C12P1/00 , C12M1/12 , C12M1/40 , C12N1/12 , C12P13/22
摘要： PURPOSE:To make possible to directly use biocatalyst without depression of activity accompanying immobilization by separating reacted solution of substrate and biocatalyst to the biocatalyst and a liquid containing substrate and reacted product by ultrafiltration membrane, the returning the former to reaction tank, etc. CONSTITUTION:Substrate is reacted with biocatalyst in aqueous solution in reaction tank 1 and the reacted solution is sent to ultrafilter 2. The reacted solution is separated to the biocatalyst and a solution containing reacted product and residual substrate by ultrafiltration membrane in the ultrafilter, then the former is returned to the reaction tank 1 and the latter is sent to crystallizing tank 3. The reacted product is crystallized in the crystallizing tank 3 and a solution containing the crystallized substance and the substrate is sent to a filter 4. Then, filtered solution containing the substrate is returned to the reaction tank 1 and crystallized reacted product is accumulated in the filter 4. Said process is preferably performed for transaminase as biocatalyst, phenylpyruvic acid and L-asparagine acid as substrate and L-phenylalanine as reacted product.

4. 发明专利

JPS60133883A NOVEL MICROORGANISM AND PRODUCTION OF L-ASPARTIC ACID USING THE SAME 失效
公开(公告)号：JPS60133883A
公开(公告)日：1985-07-17
申请号：JP24178983
申请日：1983-12-20
申请人： TANABE SEIYAKU CO
发明人： KIZUMI MASAHIKO , KOMATSUBARA SABUROU , NISHIMURA NORIYUKI , TANIGUCHI TOMOYASU
IPC分类号： C12N15/09 , C12N1/20 , C12N9/88 , C12P13/20 , C12R1/19
摘要： PURPOSE:To obtain industrially and advantageously the titled compound, by reacting fumaric acid and NH3 with microbial cells of a microorganism, obtained by integrating DNA of a miroorganism of the genus Escherichia carrying genetic information on aspartase in a vector plasmid, and incorporating the resultant integrated plasmid into a specific variant strain. CONSTITUTION:A deoxyribonucleic acid carrying the genetic information on aspartase collected from a microorganism belonging to the genus Escherichia is incorporated in a variant strain, derived from a microorganism of the genus Escherichia, and having a high aspartase activity capable of growing well in a culture medium containing L-asphartic acid as only a nitrogen source. Fumaric acid and ammonia are reacted with the resultant microbial cells, a culture or treated microbial cells thereof to afford the aimed compound.

5. 发明专利

JPS59113895A Preparation of l-aspartic acid 失效
标题翻译： L-阿片酸的制备
公开(公告)号：JPS59113895A
公开(公告)日：1984-06-30
申请号：JP21299882
申请日：1982-12-03
申请人： Tanabe Seiyaku Co Ltd
发明人： SENHATA ICHIROU , KIZUMI MASAHIKO , NISHIMURA NORIYUKI
IPC分类号： C12P13/20 , C12R1/185
CPC分类号： C12P13/20
摘要： PURPOSE: To prepare L-aspartic acid in high efficiency, by using a microbial strain having high aspartase activity and prepared by the mutagenic treatment of microorganism belonging to Escherichia genus.
CONSTITUTION: A microbial strain belonging to Escherichia genus and having aspartase activity, e.g. Escherichia coli ATCC 11303, is subjected to the conventional mutagenic treatment to obtain a mutant growing in a medium containing L-aspartic acid as sole nitrogen source or nitrogen and carbon source and having high aspartase activity, e.g. Escherichia coli EAPC-28 (FERM-BP 388) is cultured, and the cultured liquid, cultured microbial cells or treated cells are brought into contact with fumaric acid and ammonia.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：通过使用具有高天冬氨酸活性并通过诱变处理大肠埃希菌属微生物制备的微生物菌株，高效地制备L-天冬氨酸。构成：属于埃希氏菌属并具有天冬氨酰酶活性的微生物菌株，例如，对大肠杆菌ATCC11303进行常规诱变处理以获得在含有L-天冬氨酸作为唯一氮源或氮源和碳源并且具有高天冬氨酸酶活性的培养基中生长的突变体，例如，培养大肠杆菌EAPC-28（FERM-BP388），使培养的液体，培养的微生物细胞或经处理的细胞与富马酸和氨接触。

6. 发明专利

JPS53145982A PURIFICATION OF UROKINASE 失效
公开(公告)号：JPS53145982A
公开(公告)日：1978-12-19
申请号：JP6027377
申请日：1977-05-23
申请人： TANABE SEIYAKU CO
发明人： SENHATA ICHIROU , TOSA TETSUYA , SATOU TADASHI , KAKIMOTO TOSHIO , SHIBATANI TAKEYA , NISHIMURA NORIYUKI
IPC分类号： C12N9/72
摘要： PURPOSE:To prepare high purity urokinase by treating a crude urokinase solution with a beads-like gel of specific polysaccharide, and eluting the adsorbed urokinase.

7. 发明专利

JPS53136581A PURIFICATION OF UROKINASE 失效
公开(公告)号：JPS53136581A
公开(公告)日：1978-11-29
申请号：JP4963477
申请日：1977-04-28
申请人： TANABE SEIYAKU CO
发明人： SENHATA ICHIROU , KAKIMOTO TOSHIO , SHIBATANI TAKEYA , KAKIE YOSHIAKI , NISHIMURA NORIYUKI
IPC分类号： C12N9/72
摘要： PURPOSE:To prepare a highly pure urolinase suitable for the application to the human body, with industrial scale and economically, from crude urokinase, by the use of a specific polysaccharide as an adsorbent.

8. 发明专利

JPS53127885A PURIFICATION OF UROKINASE 失效
公开(公告)号：JPS53127885A
公开(公告)日：1978-11-08
申请号：JP4068377
申请日：1977-04-09
申请人： TANABE SEIYAKU CO
发明人： SENHATA ICHIROU , KAKIMOTO TOSHIO , SHIBATANI TAKEYA , KAKIE YOSHIAKI , NISHIMURA NORIYUKI
IPC分类号： C12N9/72
摘要： PURPOSE:To prepare high purity urokinase economically by adsorbing pyrogenic materials from crude urokinase using a water-insoluble polysaccharide having 3-sulfothio-2-hydroxpropyl group as adsorbent.

9. 发明专利

JPS60130389A NOVEL BACTERIUM AND PREPARATION OF L-ASPARTIC ACID USING IT 失效
公开(公告)号：JPS60130389A
公开(公告)日：1985-07-11
申请号：JP23869783
申请日：1983-12-16
申请人： TANABE SEIYAKU CO
发明人： KIZUMI MASAHIKO , NISHIMURA NORIYUKI , TAKAGI TSUTOMU
IPC分类号： C12N15/09 , C12N1/20 , C12N9/88 , C12P13/20 , C12R1/425
摘要： PURPOSE:To obtain L-aspartic acid efficiently, by transferring a hybrid plasmid having DNA carrying a genetic information of aspartase to a bacterium having high aspartase activity, released from catabolite inhibition, using this novel bacterium. CONSTITUTION:A DNA carrying genetic information of aspartase obtained from a bacterium such as Serratia marcescens Sr41, Serratia marcescens OUT8529, etc. belonging to the genus Serratia is integrated into a vector plasmid such as pACYC177, 184, etc., and the prepared hybrid plasmid is transferred to a strain, a host bacterium such as Serratia marcescens APc-494 (FERM-P 392), etc. belonging to the genus Serratia, released from catabolite inhibition. A culture solution of the bacterium thus transformed, its mold, or a treated material of it is reacted with fumaric acid and ammonia to give L-aspartic acid. The bacterium released from catabolite inhibition is obtained by subjecting a strain belonging to the genus Serratia having aspartase activity to variation and induction treatment.

10. 发明专利

JPS59113887A Preparation of l-aspartic acid 失效
标题翻译： L-阿片酸的制备
公开(公告)号：JPS59113887A
公开(公告)日：1984-06-30
申请号：JP21588982
申请日：1982-12-08
申请人： Tanabe Seiyaku Co Ltd
发明人： SENHATA ICHIROU , KIZUMI MASAHIKO , NISHIMURA NORIYUKI
IPC分类号： C12N1/20 , C12P13/20 , C12R1/425
CPC分类号： C12R1/43 , C12P13/20
摘要： PURPOSE: To prepare L-aspartic acid, in high efficiency, by using a novel microbial strain having high aspartase activity and release catabolyte repression, and prepared by the mutagenic treatment of microorganism belonging to Serratia genus.
CONSTITUTION: A microbial strain belonging to Serratia genus, having aspartase activity and sensitive to catabolyte repression, e.g. Serratia marcescens 800 is subjected to the mutagenic treatment by conventional means, and cultured in a medium containing L-aspartic acid as the sole nitrogen source or nitrogen and carbon source. The obtained novel microbial strain having high aspartase activity and released catabolyte repression, e.g. Serratia marcescens AP
c -494 (FERM-BP 392), the culture liquid obtained by the cultivation of said microbial strain, the cultured cell, or treated cell, is made to contact with fumaric acid and ammonia.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：通过使用具有高天冬氨酸酶活性和释放多巴胺降解抑制作用的新型微生物菌株，并通过诱变处理沙雷氏菌属微生物制备高效率L-天冬氨酸。构成：属于沙雷氏菌属的微生物菌株，具有天冬氨酰胺酶活性并对嗜碱性蛋白酶抑制敏感。粘质沙雷氏菌800通过常规方法进行诱变处理，并在含有L-天冬氨酸作为唯一氮源或氮和碳源的培养基中培养。获得的具有高天冬氨酸酶活性和释放的克拉酵母抑制的新型微生物菌株，例如，将粘质沙雷氏菌APc-494（FERM-BP 392），将通过培养所述微生物菌株，培养细胞或经处理的细胞获得的培养液与富马酸和氨接触。

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