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1. 发明专利

JPS62135493A CARCINONECROTIC FACTOR 失效
公开(公告)号：JPS62135493A
公开(公告)日：1987-06-18
申请号：JP27539285
申请日：1985-12-06
申请人： TANABE SEIYAKU CO
发明人： KATO JOJI , YAMAMOTO KOZO , KOMATSUBARA SABURO , TAKAGI TSUTOMU
IPC分类号： C12N15/09 , C07H21/02 , C07H21/04 , C07K14/00 , C07K14/52 , C07K14/525 , C12N1/16 , C12N1/20 , C12N5/10 , C12P21/02 , C12R1/19 , C12R1/865 , C12R1/91
摘要： NEW MATERIAL:A compound of the formula (R is a polypeptide which is composed of 16 or less amionoacids). USE:Carcinostatic agent. PREPARATION:For example, A lipopolysaccharide originating from a microorganism is added macrophage cells originating from mouse and they are cultured. Then, the culture mixture is centrifuged to separate cells. The cells are broken with guanidine isocyanate and stacked on a cesium chloride solution, then centrifuged to isolate RNA. The RNA is treated with oligo(dT) cellulose to separate poly(A)RNA fraction and subjected to sucrose density-gradient centrifugation to concentrate and purify mRNA. The product is used as a template to form cDNA with a reverse transcriptase. Then, the cDNA is bonded to an appropriate vector to form a recombinant plasmid, which is inserted into Escherichea coli to effect transformation. Then, the transformed microorganism is cultured and the compound of the formula is separated from the culture mixture.

2. 发明专利

JPS61249395A M-RNA AND ITS PREPARATION 失效
公开(公告)号：JPS61249395A
公开(公告)日：1986-11-06
申请号：JP9213485
申请日：1985-04-26
申请人： TANABE SEIYAKU CO
发明人： KATO JOJI , KOMATSUBARA SABURO , TAKAGI TSUTOMU
IPC分类号： C12N15/09 , A61K31/70 , A61P35/00 , C07H21/02 , C12N15/00
摘要： PURPOSE:Mammarian cells which are capable of producing cancer cell-injuring substance is cultured in the presence of a stimulator to give m-RNA (messenger-RNA) coding cancer cell-injuring substance. CONSTITUTION:Cells of a mammarian animal such as mouse, rat, rabbit, cat, dog, monkey, cattle, horse or human, preferably rinsed cells of rabbit alveoli, macrofage cell strain J774.1 are cultured in the presence of a stimulator to accumulate m-RNA coding cancer cell-injuring substance in the cells. Then, the objective m-RNA is separated from the cells.

3. 发明专利

JPS589692A PRODUCTION OF L-ARGININE THROUGH FERMENTATION PROCESS 失效
公开(公告)号：JPS589692A
公开(公告)日：1983-01-20
申请号：JP10474681
申请日：1981-07-03
申请人： TANABE SEIYAKU CO
发明人： SENHATA ICHIROU , KIZUMI MASAHIKO , TAKAGI TSUTOMU
IPC分类号： C12P13/10 , C12R1/425
摘要： PURPOSE:A strain in Serratia, requiring succinic acid or resisting nucleic acid base analogs, and producing the titled substance, is cultured and the titled substance is collected from the culture mixture. CONSTITUTION:A mutant in Serratia which requires succinic acid or resists nucleic acid base analogs such as Serratia marcescens SA268 or Cerratia marcescens AUr-116 is cultured in a medium for L-arginine production. The culture medium contains glucose, fumaric acid, alcohol, ammonium sulfate, urea, pepton, fish meat extract, inorganic salts and, when a succininc acid-requiring mutant is employed, further sodium succinate, ammonium succinate or other analogous salt is added. The cultivation is carried out aerobically at 20-40 deg.C and 6-8pH and, after completion of cultivation, insoluble substances are removed, then separation and purification are conducted by means of ion-exchange resins to give L-arginine.

4. 发明专利

JPH11346772A SCREENING OF NEURONAL DEATH INHIBITOR 失效
公开(公告)号：JPH11346772A
公开(公告)日：1999-12-21
申请号：JP15978098
申请日：1998-06-09
申请人： TANABE SEIYAKU CO , TOYAMA MASAYA
发明人： IMAIZUMI KAZUNORI , TSUDA MANABU , KATAYAMA TAIICHI , TAKAGI TSUTOMU , TOYAMA MASAYA
IPC分类号： G01N33/15 , A61K38/00 , A61K45/00 , A61P25/28 , C12N15/00 , C12N15/09 , C12P21/02 , C12Q1/68 , C12R1/91
摘要： PROBLEM TO BE SOLVED: To screen medicines inhibiting neuronal death by examining the inhibitory action of specimen substances on binding of DP5 protein or its homologous protein to a protein belonging to Bcl-2 family. SOLUTION: Neuronal death inhibitors having the inhibitory action on neuronal death and useful e.g. for screening medicines to be used e.g. as therapeutic drugs for neuronal degenerative disorders, such as Alzheimer's disease are screened by examining the inhibitory action of specimen substances on binding of DP5 protein or its homologous protein having the ability to bind to a protein belonging to Bcl-2 family and the action of inducing programmed cell death in neurons to a protein such as Bcl-2 or Bcl-XL belonging to Bcl-2 family and having an inhibitory action of programmed cell death in neurons.

5. 发明专利

JPH09238685A CEREBRAL ISCHEMIA-RELATED GENE 失效
公开(公告)号：JPH09238685A
公开(公告)日：1997-09-16
申请号：JP4938096
申请日：1996-03-07
申请人： TANABE SEIYAKU CO , TOYAMA MASAYA
发明人： TOYAMA MASAYA , TAKAGI TSUTOMU , IMAI YUJI , IMAIZUMI KAZUNORI
IPC分类号： C12N15/02 , C07K14/47 , C07K16/00 , C07K16/18 , C12N1/21 , C12N5/10 , C12N15/09 , C12P21/08
摘要： PROBLEM TO BE SOLVED: To obtain the subject new gene coding for a polypeptide having a specific amino acid sequence, to be induced in astrocytes when exposed to a hypoxic condition such as cerebral ischemia-reperfusion followed by being exposed to oxygen again, thus useful for preventing/treating cerebral ischemic diseases. SOLUTION: This gene is a new cerebral ischemia-related gene containing a base sequence coding for a polypeptide composed of a amino acid sequence of the formula. This gene is expressed and induced in astrocytes when exposed to a hypoxic condition such as cerebral ischemia-reperfusion followed by being exposed to oxygen again, therefore being useful for preventing/treating cerebral ischemic diseases of producing new polypeptides. This gene is obtained by the following process: a cerebral hemisphere is extracted from a SD-strain rat just after born and then put to enzymegenation to isolate cells which, in turn, are cultured in a medium spiked with cytosine arabinofuranoside and astrocytes are separated followed by isolating mRNA by conventional method, and the mRNA is subjected to PCR using a reverse trnascriptase and a primer to effect cloning.

6. 发明专利

JPH09107971A SEARCH OF GENE 失效
公开(公告)号：JPH09107971A
公开(公告)日：1997-04-28
申请号：JP27082295
申请日：1995-10-19
申请人： TANABE SEIYAKU CO , TOYAMA MASAYA
发明人： TOYAMA MASAYA , TAKAGI TSUTOMU , SUZUKI YUTAKA , SATO NAOYA
IPC分类号： G01N33/53 , C07H21/02 , C07H21/04 , C07K14/47 , C07K14/52 , C07K16/00 , C07K16/18 , C12N1/21 , C12N15/02 , C12N15/09 , C12P21/02 , C12P21/08 , C12Q1/68 , C12R1/19 , C12R1/91 , G01N33/531 , G01N33/577
摘要： PROBLEM TO BE SOLVED: To provide a method for efficiently searching and finding out a gene different in manifestation amounts between plural organism specimens and to obtain both a new polypeptide participating in differentiation of nerve cell and a DNA coding for polypeptide. SOLUTION: This method for searching a gene comprises exploring a gene different in manifestation amounts between plural organism specimens through (1) a process for preparing a double-strand cDNA pool of a standard organism specimen and uniforming the content of each DNA fragment in the pool, (2) a process for preparing a double-strand cDNA pool derived from each specimen concerning plural organism specimen to be mutually compared and contrasted and (3) a process for removing a fragment which is associated with a DNA fragment in the cDNA pool obtained by the process (2) from the standard cDNA pool having the uniform content obtained by the process (1).

7. 发明专利

JPS59183688A Preparation of l-aspartic acid 失效
标题翻译： L-阿片酸的制备
公开(公告)号：JPS59183688A
公开(公告)日：1984-10-18
申请号：JP5722983
申请日：1983-03-31
申请人： Tanabe Seiyaku Co Ltd
发明人： KIZUMI MASAHIKO , TAKAGI TSUTOMU
IPC分类号： C12N1/20 , C12N9/88 , C12N15/09 , C12P13/20
CPC分类号： C12P13/20 , C12N9/88
摘要： PURPOSE: To produce L-aspartic acid economically or an industrial scale, by preparing a microbial strain having high aspartase activity by self-cloning technique, and contacting the microorganisms with furamic aid and ammonia.
CONSTITUTION: A deoxyribonucleic acid carrying the gene information of aspartase and separated from microorganisms belonging to Serratia genus is integrated in a plasmid, and the obtained hydbrid plasmid is inserted into a microorganism belonging to Serratia genus. The obtained microbial strain is made to contact with fumaric acid and ammonia. The cultured liquid of the microorganism, the microbial cell collected from the liquid, or treated microbial cell may be used in place of the microbial cell itself.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：通过自身克隆技术制备具有高天冬氨酸活性的微生物菌株，并将微生物与呋虫胺和氨接触，从而经济地或者工业规模生产L-天冬氨酸。构成：携带天冬氨酸酶的基因信息并从属于沙雷氏菌属的微生物分离的脱氧核糖核酸整合入质粒中，将得到的杂交质粒插入属于沙雷氏菌属的微生物中。使得到的微生物菌株与富马酸和氨接触。可以使用微生物的培养液，从液体收集的微生物细胞或经处理的微生物细胞代替微生物细胞本身。

8. 发明专利

JPS5860995A PREPARATION OF L-PROLINE BY FERMENTATION 失效
公开(公告)号：JPS5860995A
公开(公告)日：1983-04-11
申请号：JP15982981
申请日：1981-10-06
申请人： TANABE SEIYAKU CO
发明人： SENHATA ICHIROU , KIZUMI MASAHIKO , SUGIURA MASAKI , TAKAGI TSUTOMU
IPC分类号： C12P13/24 , C12R1/425
摘要： PURPOSE:To form and to accumulate the titled substance and to collect it, by cultivating a bacterium capable of producing L-proline, lacking in L-proline degrading enzyme, having resistance to proline analogue and/or purine analogue. CONSTITUTION:A bacterium such as Serratia marcescens, etc. belonging to the genus Serratia, capable of producing L-proline, lacking in L-proline degrading enzyme (e.g., lacking in proline oxidase), having a resistance to proline analogue and/or purine analogue, is cultivated. The medium consists of 10-20wt% carbon source, 1-5wt% nitrogen source, 0-1wt% organic nutrient substance, etc. and kept at a pH of 6-8. The strain is cultivated in the medium under aerobic conditions for 2-7 days, and accumulated L-proline is collected by a common separating and purifying operations using an ion exchange resin etc.

9. 发明专利

JPS589693A PRODUCTION OF L-GLUTAMIC ACID THROUGH FERMENTATION PROCESS 失效
公开(公告)号：JPS589693A
公开(公告)日：1983-01-20
申请号：JP10474581
申请日：1981-07-03
申请人： TANABE SEIYAKU CO
发明人： SENHATA ICHIROU , KIZUMI MASAHIKO , TAKAGI TSUTOMU
IPC分类号： C12P13/14 , C12R1/425
摘要： PURPOSE:A strain producing L-glutamic acid, which belongs to Serratia marcescens and resists delta-hydroxylysine or requires succinic acid, is cultured and the titled substance is collected from the culture mixture. CONSTITUTION:A parent strain such as Serratia marcescens HY is treated with a mutation inducer to cause mutation and give a L-glutamic acid-producing strain that resists hydroxylysine or requires succinic acid such as Serratia marcescence AHr-30 or Serratia marcescens SUC-100. The resultant mutant is aerobically cultured in a medium containing glucose, succinic acid, ammonium chloride, urea, pepton, potassium phosphate and so on. After completion of cultivation, bacteria cells are removed, then isolation and purification are carried out by means of ion-exchange resins to collect L-glutamic acid.

10. 发明专利

JP2000093043A LIG-1 GENE DELETION ANIMAL 失效
公开(公告)号：JP2000093043A
公开(公告)日：2000-04-04
申请号：JP26613698
申请日：1998-09-21
申请人： TANABE SEIYAKU CO , TOYAMA MASAYA
发明人： TAKAGI TSUTOMU , SUZUKI YUTAKA , TOYAMA MASAYA
IPC分类号： C12N15/09 , A01K67/027 , A61K31/00 , A61K45/00 , A61P17/00 , A61P17/06
摘要： PROBLEM TO BE SOLVED: To provide a screening method of a therapeutic medicine and a preventing medicine for skin diseases, comprising assaying whether or not a substance to be examined has a suppressing effect, on skin lesion by administering the substance to be examined to an animal in which function of LIG-1 gene is lost. SOLUTION: A nonhuman animal, preferably a rodent animal e.g. mouse, or its progeny in which a function of a variant type LIG-1 gene, in which neomycin-resistant gene is inserted preferably into an exon containing translation initiation codon, is lost is prepared.

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