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1. 发明专利

JPH0638787A SOLUBLE ERYTHROPOIETIN RECEPTOR PROTEIN AND GENE CODING ITS AMINO ACID SEQUENCE 失效
公开(公告)号：JPH0638787A
公开(公告)日：1994-02-15
申请号：JP8286592
申请日：1992-03-04
申请人： SNOW BRAND MILK PROD CO LTD
发明人： SASAKI RYUZO , NAGAO MASAYA , MASUDA SEIJI , UEDA MASAJI
IPC分类号： C12N15/09 , C12N15/12 , C12P21/02
摘要： PURPOSE:To obtain a soluble erythropoietin receptor protein and a gene coding its amino acid sequence. CONSTITUTION:A DNA sequence having a region from a transmembrane region of gene coding an amino acid sequence of erythropoietin receptor protein to the N end side and its variant. A soluble erythropoietin receptor protein expressed by this DNA sequence, having a binding property in erythropoietin and having an organic antigenicity as erythropoietin receptor. This protein has characteristics as a EPO binding property and EPO receptor antigen and is useful as a medicine, a diagnostic and a biochemical reagent.

2. 发明专利

JPH02215398A BIOLOGICAL ACTIVITY ASSAY OF ERYTHROPOIETIN 失效
公开(公告)号：JPH02215398A
公开(公告)日：1990-08-28
申请号：JP3793789
申请日：1989-02-17
申请人： SNOW BRAND MILK PROD CO LTD
发明人： TSUDA HIDEYORI , AKAI KUNIHISA , UEDA MASAJI
IPC分类号： G01N33/74 , C07K14/00 , C07K14/505 , C07K14/52 , C12Q1/04
摘要： PURPOSE:To accomplish improvement of the accuracy of the title assay simply and safely by making a culture through mixing a specimen with a specific culture solution, adding a soluble tetrazolium salt to the system, solubilizing cells followed by color development. CONSTITUTION:Mouse myeloid cell strain EP-FDC-P2 cells living or growing depending simply on erythropoietin(EPO) and mouse interleukin III are suspended in a culture medium containing FCS, etc., along with coexistence of EPO-contg. specimen or standard EPO and put to culture in a CO2 incubator for 12-24hr. The resultant culture solution is then incorporated with a color-developable coloring matter-soluble tetrazolium salt(MTT) so as to be ca.5mg/ml in its concentration followed by reaction for 3-5hr to effect solubilization of the cells to obtain a color developed coloring matter solution. Thence, the extinction coefficient at ca.540nm of this solution is measured with a reference wavelength of ca.620nm using an instrument such as an immunoreader, thus determining the objective biological activity of erythropoietin.

3. 发明专利

JPS62185020A PRODUCTION OF COLONY STIMULATION FACTOR (CSF) 失效
公开(公告)号：JPS62185020A
公开(公告)日：1987-08-13
申请号：JP2473886
申请日：1986-02-06
申请人： SNOW BRAND MILK PROD CO LTD
发明人： MURAKAMI MASAHIKO , UEDA MASAJI , TAKAHASHI MASATOMO , MIZOGUCHI HIDEAKI
IPC分类号： A61K35/12 , A61K35/13 , A61K38/16 , A61K38/17
摘要： PURPOSE:To enable the mass-production of CSF, by establishing CSF-producing cell strain able to be subcultured over a long period from a gastric tumor tissue exhibiting neutrophilia free from symptom of hypercalcemia and culturing said cell strain. CONSTITUTION:Tumor cell is extracted from a gastric carcinoma patient exhibiting neutrophilia free from symptom of hypercalcemia and transplanted to a nude mouse. The cell is subcultured for several generations and a strain exhibiting leukocytosis in peripheral blood of nude mouse is selected. A thin section of the tumor tissue is subjected to monolayer cell culture by dispase treatment and coexisting nude mouse-originated cells are removed by the use of a rabbit antiserum against fibroblast cell originated from cultured nude mouse spleen. The strain is subcultured for 12 generations and cloned by limiting dilution method to establish a CSF-producing cell strain. The cell strain is cultured in a serum-containing medium until the system reaches a confluent state, when the medium is exchanged to a serum-free medium. CSF is produced by culturing the strain in the serum-free medium.

4. 发明专利

JPS59148725A Preparation of high-purity human transferrin 失效
标题翻译：高纯度人转铁蛋白的制备
公开(公告)号：JPS59148725A
公开(公告)日：1984-08-25
申请号：JP2163983
申请日：1983-02-14
申请人： Snow Brand Milk Prod Co Ltd
发明人： UEDA MASAJI , GOTOU MASAAKI , TAKESHITA YASUYOSHI
IPC分类号： C07K16/00 , A61K35/14 , A61K35/16 , A61K38/16 , A61K39/395 , C07K1/22 , C07K14/435 , C12P21/08
摘要： PURPOSE: To obtain the titled substance having high immunospecific purity, in high efficiency by single absorption and desorption process, by using an adsorbent bonded with a monoclonal antibody obtained from a hybridoma cell produced by cell fusion.
CONSTITUTION: The spleen cell of a mouse immunized with human transferrin (Tf) is fused with a myeloma cell by cell fusion to obtain a hybridoma cell, from which a monoclonal anti-human-Tf antibody is produced. The antibody is bonded to an adsorbent [e.g. Affigel (product of Biolad Corp.), etc.], and the obtained adsorbent is made to contact with human serum or plasma to effect the adsorption of human Tf. The adsorbed human Tf is desorbed to obtain a high- purity human Tf. Since the antibody in the adsorbent bonded with antibody is a monoclonal antibody of human Tf, the quality of the antibody is uniform, the hybridoma cell producing said antibody can be preserved in frozen state, and the antibody is available easily and stably. The used adsorption column can be regenerated and reused about 10 times.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：通过使用与通过细胞融合产生的杂交瘤细胞获得的单克隆抗体结合的吸附剂，通过单次吸收和解吸过程高效地获得具有高免疫特异性纯度的标题物质。构成：用人转铁蛋白（Tf）免疫的小鼠的脾细胞通过细胞融合与骨髓瘤细胞融合，得到杂交瘤细胞，由此产生单克隆抗人Tf抗体。抗体结合到吸附剂[例如， Affigel（Biolad Corp.的产品）等]，使得到的吸附剂与人血清或血浆接触以实现人Tf的吸附。吸附的人Tf被解吸以获得高纯度人Tf。由于与抗体结合的吸附剂中的抗体是人Tf的单克隆抗体，所以抗体的质量是均匀的，产生所述抗体的杂交瘤细胞可以保存在冷冻状态，并且抗体可以容易且稳定地获得。使用的吸附塔可以再生和重复使用约10次。

5. 发明专利

JPS57163482A NOVEL YEAST HAVING SPECIFIC SACCHARIDE UTILIZATION 失效
公开(公告)号：JPS57163482A
公开(公告)日：1982-10-07
申请号：JP4888381
申请日：1981-03-31
申请人： SNOW BRAND MILK PROD CO LTD
发明人： MIYATA NOBUO , HIROTA TETSUJI , UEDA MASAJI , IWAZAWA SHINJI , AHIKO KENKICHI
IPC分类号： C12N1/16 , C12R1/88
摘要： PURPOSE:A yeast, having a galactokinase as a constituent enzyme, and the utilization of galactose in preference to that of glucose, and incapable of utilizing fructose though capable of utilizing the glucose due to the absence of hexonase. CONSTITUTION:A yeast, having glucokinase and galactokinase as a constituent enzyme, deficient in hexokinase, and having the properties of utilizing galactose preferentially in a mixed saccharide solution containing the galactose even in the previous cultivation in a culture medium containing a utilizable saccharide other than the galactose. The mycological properties of Torulopsis holmii KY-5 (FERM-P No 5766) which is this yeast are most closely resemble the Torulopsis holmii except the deficiency in fermentation property of fructose and reffinose. The yeast is found from kefir globules which are the seed yeasts of fermented milk kefir.

6. 发明专利

JP2560069B2 失效
公开(公告)号：JP2560069B2
公开(公告)日：1996-12-04
申请号：JP5578288
申请日：1988-03-09
申请人： SNOW BRAND MILK PROD CO LTD
发明人： GOTO MASAAKI , MURAKAMI MASAHIKO , AKAI KUNIHISA , UEDA MASAJI
IPC分类号： C12N15/02 , C07K1/22 , C07K14/00 , C07K14/505 , C07K14/52 , C07K16/00 , C07K16/18 , C12N5/00 , C12N5/10 , C12N15/00 , C12P21/00 , C12P21/02 , C12P21/08 , C12R1/91

7. 发明专利

JPH0592928A THERAPEUTIC AGENT FOR DYSMNESIA 失效
公开(公告)号：JPH0592928A
公开(公告)日：1993-04-16
申请号：JP27825291
申请日：1991-09-30
申请人： SNOW BRAND MILK PROD CO LTD
发明人： SASAKI RYUZO , UEDA MASAJI
IPC分类号： A61K38/22 , A61P25/28
摘要： PURPOSE:To obtain a therapeutic agent for dysmnesia capable of raising the intracellular calcium level of neurons, enhancing choline acetyltransferase activity and preventing, improving or treating Alzheimer type dysmnesia. CONSTITUTION:A therapeutic agent for dysmnesia containing erythropoietin(EPO) protein which is one kind of cytokinin and a hematopoietic factor, preferably the EPO or EPO mutein protein prepared by a genetic engineering technique as an active ingredient. The above-mentioned therapeutic agent is a preventive, improving or therapeutic agent for Alzheimer type dysmnesia in the dosage form of preferably a direct infusion into intracranial septal areas. The preventive, improving and therapeutic agent acts as a neurotrophic factor for neurons and has the preventive, improving and therapeutic actions on the dysmnesia. Since toxicity is low, the agent can safely be used. Furthermore, although the dose of the above-mentioned active ingredient can suitably be changed according to the extent of damage, it is about 1000-100000 IU at a time for an adult.

8. 发明专利

JPH01228492A PRODUCTION OF ERYTHROPOIETIN 失效
公开(公告)号：JPH01228492A
公开(公告)日：1989-09-12
申请号：JP5578288
申请日：1988-03-09
申请人： SNOW BRAND MILK PROD CO LTD
发明人： GOTO MASAAKI , MURAKAMI MASAHIKO , AKAI KUNIHISA , UEDA MASAJI
IPC分类号： C12N15/02 , C07K1/22 , C07K14/00 , C07K14/505 , C07K14/52 , C07K16/00 , C07K16/18 , C12N5/00 , C12N5/10 , C12N15/00 , C12P21/00 , C12P21/02 , C12P21/08 , C12R1/91
摘要： PURPOSE:To enable isolation of erythropoietin of high purity by conducting adsorption and elution using monoclonal(MoC) antierythropoietin(EPO) antibody which is obtained by giving a specific hybridoma. CONSTITUTION:Hybridoma cells (HB) are obtained by effecting cell fusion between spleen cells of mouse or the like, immunized with antigen EPO of higher than 60wt.% purity and myeloma cells. The HB cells are screened by bonding it to 1-EPO to obtain anti-EPO antibody-producing HB. The antibody- producing HB is given peritoneally to a mouse to produce the antibody, then the ascites is fractionated with ammonium sulfate and columns to prepare monoclonal anti-EPO antibody. The sample containing EPO is adsorbed on an adsorbent to which the EPO antibody is bonded, such as affinity gel 10, and the EPO is eluted a mixed solution of acetic acid and sodium chloride to collect erythropoietin of high purity.

9. 发明专利

JPS59116297A Preparation of high purity erythropoietin 失效
标题翻译：高纯度红细胞生成素的制备
公开(公告)号：JPS59116297A
公开(公告)日：1984-07-05
申请号：JP23153982
申请日：1982-12-23
申请人： Snow Brand Milk Prod Co Ltd
发明人： CHIBA HIDEO , SASAKI RIYUUZOU , YANAGAWA SHINICHI , UEDA MASAJI
IPC分类号： G01N33/53 , C07G15/00 , C07K1/14 , C07K14/00 , C07K16/00 , C12N5/00 , C12N5/18 , C12N15/00 , C12N15/02 , C12P21/00 , C12P21/08 , G01N33/543 , G01N33/577
摘要： PURPOSE: To prepare the titled compound useful as an erythropoiesis promoting factor, in high efficiency, by preparing a variety of monoclonal antibodies by the immunization with the whole urine protein of a patient of hypoplastic anemia followed by the cell fusion, bonding the antibodies to a carrier and contacting the obtained adsorbent with the urine of the patient of hypoplastic anemia, etc.
CONSTITUTION: The spleen cells of an experiment animal immunized with the whole urine protein of a patient of hypoplastic anemia is subjected to the cell fusion with myeloma cell to prepare a variety of monoclonal antibodies, which are bonded to a carrier to prepare an adsorbent. The adsorbent is packed in a column, and made to contact with the urine of the patient of hypoplastic anemia or its treated product. The urine proteins other than erythropoietin are adsorbed and removed by this treatment, and the objective high-purity erythropoietin eluted from the column as the unadsorbed fraction is collected.
COPYRIGHT: (C)1984,JPO&Japio
摘要翻译：目的：为了高效制备可用作红细胞生成促进因子的标题化合物，可以通过免疫接种细胞融合后的发育不全的贫血患者的全尿蛋白进行免疫制备各种单克隆抗体，将抗体结合到载体并使所获得的吸附剂与患有再发性贫血的患者的尿液接触等。构成：用异常性贫血患者的全尿蛋白免疫的实验动物的脾细胞与骨髓瘤细胞进行细胞融合，制备多种单克隆抗体，其与载体结合以制备吸附剂。将吸附剂装入柱中，使其与发育不全的贫血患者的尿液或其处理过的产品接触。除了红细胞生成素之外的尿蛋白被该处理吸附除去，并且收集从柱中洗脱的目的高纯度促红细胞生成素作为未吸附部分。

10. 发明专利

JPS57208988A PURIFICATION OF ACIDIC CARBOXYPEPTIDASE 失效
公开(公告)号：JPS57208988A
公开(公告)日：1982-12-22
申请号：JP9266181
申请日：1981-06-16
申请人： SNOW BRAND MILK PROD CO LTD
发明人： IWAZAWA SHINJI , UEDA MASAJI , MIYATA NOBUO , AHIKO KENKICHI
IPC分类号： C12N9/48 , C12R1/80
摘要： PURPOSE:To purify acidic carboxypeptidase, in an industrial scale, by passing acidic carboxypeptidase prepared by the cultivation of microorganisms through a column packed with carboxy-sephadex. CONSTITUTION:A bacterial strain capable of producing acidic carboxypeptidase and belonging to Penicillium genus, e.g. Penicillium caseicolum, is cultured, and the filtrate of the cultured product is passed through a column packed with carboxy-sephadex at an ionic strength of =0.5M and a pH of 3.0-4.0.

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