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1. 发明专利

JP2006267432A Scanning type viewing apparatus 审中-公开
标题翻译：扫描式查看装置
公开(公告)号：JP2006267432A
公开(公告)日：2006-10-05
申请号：JP2005084262
申请日：2005-03-23
申请人： Olympus Corp , オリンパス株式会社
发明人： NAGASAWA NOBUYUKI
IPC分类号： G02B21/06 , G02B26/10
摘要： PROBLEM TO BE SOLVED: To quickly perform alignment of an objective lens for a specimen in a preparatory stage by allowing a simple and accurate confirmation of the optical axis position of the objective lens relative to a part desired to be viewed on the specimen.
SOLUTION: This scanning type viewing apparatus 1 comprises: a first light source 2; an optical scanning section 3 that scans light L
1 from the first light source 2 over a specimen A; an objective lens 6 that forms an image on the specimen A with the light L
1 scanned in the optical scanning section 3; an optical detecting section 9 that detects the return light L
2 emitted from the specimen A; a second light source 12 for emitting visible light L
3 ; a deflective optical element 16 that is arranged between the optical scanning section 3 and the objective lens 6 and that makes the visible light L
3 emitted from the second light source 12 enter the objective lens 6 along the optical axis C of the objective lens 6; and a beam shaping section 14 in which the visible light L
3 from the second light source 12 to be emitted on the surface of the specimen A through the objective lens 6 is formed, by the deflective optical element 16, into patterns P
1 -P
3 that indicate the optical axis C of the objective lens 6.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：通过简单准确地确认物镜的光轴位置相对于期望在样本上观察的部分，能够在准备阶段中快速地进行用于样品的物镜的对准。解决方案：该扫描型观察装置1包括：第一光源2; 在第一光源2上经过试样A扫描光L 1的光学扫描部3; 在光学扫描部分3中扫描的光L 1 在样本A上形成图像的物镜6; 光检测部9，检测从检体A发出的返回光L 2 用于发射可见光L 3的第二光源12; 布置在光学扫描部分3和物镜6之间并使得从第二光源12发射的可见光L <3> 的偏转光学元件16沿光轴进入物镜6 C; 并且通过偏转光学元件16形成其中通过物镜6在样品A的表面上发射来自第二光源12的可见光L 3 SB 3的光束成形部分14 ，表示指示物镜6的光轴C的图案P 1 3 。版权所有（C）2007，JPO＆INPIT

2. 发明专利

JP2006071544A Camera obscura device for fluorescence observation, fluorescence observation system, and fluorescence observation method 有权
标题翻译：用于荧光观察，荧光观察系统和荧光观察方法的相机用OBSCURA装置
公开(公告)号：JP2006071544A
公开(公告)日：2006-03-16
申请号：JP2004257240
申请日：2004-09-03
申请人： Olympus Corp , オリンパス株式会社
发明人： TSUYUKI HIROSHI , CHO KAZUHIKO , NAGASAWA NOBUYUKI
IPC分类号： G01N21/64 , G02B21/24
CPC分类号： G01N21/6458 , G01N21/6456
摘要： PROBLEM TO BE SOLVED: To provide a clear fluorescent picture by removing noise in a fluorescent picture obtained in fluorescence observation while checking relative positional relation between a fluorescence observation device and a specimen even during fluorescence observation.
SOLUTION: This camera obscura device 1 for fluorescence observation is equipped with: a camera obscura body 2 for covering the specimen A and the fluorescence observation device 5, the observation device 5 illuminating the specimen A with excitation light L1 of a first wavelength band to detect fluorescence L2 of a second wavelength band generated from the specimen A; an illumination light source 3 disposed in the obscura body 2 for outputting visible light L3 of a third wavelength band different from the first and second wavelength bands; and an observation window 4 provided in the obscura body 2 and transmitting light of a fourth wavelength band not including the first and second wavelength bands but including at least a part of the third wavelength band.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：即使在荧光观察期间，也可以在检测荧光观察装置与试样之间的相对位置关系的同时，通过去除在荧光观察中获得的荧光图像中的噪声来提供清晰的荧光图像。
解决方案：该照相机遮光装置1用于荧光观察配备有：用于覆盖样本A和荧光观察装置5的照相机遮蔽体2，观察装置5用第一波长的激发光L1照射样本A 检测从样品A产生的第二波长带的荧光L2; 设置在遮光体2中用于输出不同于第一和第二波长带的第三波长带的可见光L3的照明光源3; 以及设置在遮光体2中并且透射不包括第一和第二波长带但包括第三波长带的至少一部分的第四波长带的光的观察窗4。版权所有（C）2006，JPO＆NCIPI

3. 发明专利

JP2009257967A Fluorescence observation apparatus and fluorescence observation method 审中-公开
标题翻译：荧光观察装置和荧光观察方法
公开(公告)号：JP2009257967A
公开(公告)日：2009-11-05
申请号：JP2008108036
申请日：2008-04-17
申请人： Olympus Corp , オリンパス株式会社
发明人： NAKAJIMA CHIKA , NAGASAWA NOBUYUKI , TANIGAWA YOSHIHISA
IPC分类号： G01N21/64
CPC分类号： G01N21/6456
摘要： PROBLEM TO BE SOLVED: To clearly observe a site of interest to be observed in an image during fluorescence observation of a small experimental animal even when the fluorescence is extremely low.
SOLUTION: This fluorescent observation device 1 comprises a light source 9 for emitting excitation light, an optical system 10 for irradiating an imaging site on the small experimental animal A with the excitation light from the light source 9, a light-blocking means 11 for blocking light in a prescribed region of the small experimental animal A or in an image of the prescribed region, an imaging means 15 for taking a fluorescence image of the small experimental animal A, and a control means 5 configured to identify a high-fluorescence region having a prescribed fluorescence amount or above in the fluorescence image of the small experimental animal A acquired by the imaging means 15 and to control the light-blocking means 11 so as to block the identified high-fluorescence region.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：即使当荧光极低时，也可以清楚地观察在小型实验动物的荧光观察期间在图像中观察到的感兴趣的部位。解决方案：该荧光观察装置1包括用于发射激发光的光源9，用于用来自光源9的激发光照射小实验动物A上的成像部位的光学系统10，遮光装置 11，用于阻挡小实验动物A的规定区域的光或规定区域的图像，用于取得小型实验动物A的荧光图像的摄像单元15，以及控制单元5，在由成像装置15获取的小实验动物A的荧光图像中具有规定的荧光量以上的荧光区域，并控制遮光装置11以阻挡所识别的高荧光区域。版权所有（C）2010，JPO＆INPIT

4. 发明专利

JP2009139475A Observation apparatus 有权
标题翻译：观察装置
公开(公告)号：JP2009139475A
公开(公告)日：2009-06-25
申请号：JP2007313546
申请日：2007-12-04
申请人： Olympus Corp , オリンパス株式会社
发明人： NAGASAWA NOBUYUKI , NATORI YASUAKI
IPC分类号： G02B21/06 , G01N21/76 , G02B21/16
CPC分类号： G02B21/16 , G02B21/24
摘要： PROBLEM TO BE SOLVED: To provide an observation apparatus which prevents incidence of strong external light into a photodetector and an imaging device, while a lid is opened, thereby performing accurate photodetection and imaging operations without being affected by the external light.
SOLUTION: The observation apparatus comprises a stage 2 on which a sample A, serving as an observation target is, placed; a detection means which detects light from the sample A; an imaging optical system which projects the light from the sample A onto the detection means; a light-shielding member 6 which covers the whole or a part of them; an opening provided in the light shielding member 6; a lid 12 which opens/closes the opening; an open/close detection means 7 which detects the open/close state of the lid 12; a dimming means 4 which limits light incident into the detection means; and a controller which operates the dimming means 4 to reduce the quantity of the light incident into the detection means, when the open/close detection means 7 detects that the lid 12 has been opened.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种在盖打开的同时防止强的外部光入射到光电检测器和成像装置中的观察装置，从而在不受外部光影响的情况下进行精确的光电检测和成像操作。解决方案：观察装置包括：放置有用作观察目标的样品A的载物台2; 检测来自样品A的光的检测装置; 成像光学系统，其将来自样品A的光投射到检测装置上; 覆盖其全部或一部分的遮光构件6; 设在遮光部件6上的开口部; 打开/关闭开口的盖12; 检测盖12的打开/关闭状态的打开/关闭检测装置7; 限制入射到检测装置中的光的调光装置4; 以及当打开/关闭检测装置7检测到盖12已经打开时，操作调光装置4以减少入射到检测装置中的光量的控制器。版权所有（C）2009，JPO＆INPIT

5. 发明专利

JP2007127835A Observing apparatus 审中-公开
标题翻译：观察设备
公开(公告)号：JP2007127835A
公开(公告)日：2007-05-24
申请号：JP2005320503
申请日：2005-11-04
申请人： Olympus Corp , オリンパス株式会社
发明人： OKADA JUNICHI , NAGASAWA NOBUYUKI
IPC分类号： G02B21/00 , G02B21/36
摘要： PROBLEM TO BE SOLVED: To provide an observing apparatus which dispenses with a dedicated detecting device for detecting displacement or distortion of a lens optical system caused by external stress and is therefore prevented from becoming complex or larger. SOLUTION: The observing apparatus 1 includes; an objective optical system 3 which condenses light from a specimen A; an image capturing device 4 which captures an image by detecting light condensed by the objective optical system 3; and an image processing section 5 which processes the image captured by the image capturing device 4. The image capturing device 4 captured an image including the visual field frame of the objective optical system 3. The image processing section 5 obtains the center position of an optical axis based on the visual field frame included in the image captured by the image capturing device 4. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：解决的问题：提供一种观察装置，其省去了用于检测由外部应力引起的透镜光学系统的位移或变形的专用检测装置，从而防止变得复杂或更大。解决方案：观察装置1包括：将来自试样A的光聚光的物镜光学系统3; 通过检测被物镜光学系统3所聚光的光来拍摄图像的摄像装置4; 以及处理由图像拍摄装置4拍摄的图像的图像处理部5.图像拍摄装置4拍摄包括物镜光学系统3的视场帧的图像。图像处理部5获得光学系统的中心位置基于包括在由图像捕获装置4捕获的图像中的视野帧。版权所有：（C）2007，JPO＆INPIT

6. 发明专利

JP2006020715A Organism observation apparatus 有权
标题翻译：有机观察装置
公开(公告)号：JP2006020715A
公开(公告)日：2006-01-26
申请号：JP2004199498
申请日：2004-07-06
申请人： Olympus Corp , オリンパス株式会社
发明人： KONO YOSHIHIRO , MAGARA TAISUKE , TANIGAWA YOSHIHISA , NAGASAWA NOBUYUKI
IPC分类号： A61B1/00
CPC分类号： A61B1/07 , A61B1/00172 , A61B1/0057
摘要： PROBLEM TO BE SOLVED: To provide an organism observation apparatus capable of observing a micro observation object portion in the interior of an organism by a simple constitution.
SOLUTION: This organism observation apparatus 1 is provided with a light source 2; a flexible light transmitting member 18 propagating the light from the light source 2, illuminating it from a tip face 18a to the observation object portion, receiving a return light returning from the observation object portion in the tip face 18a and propagating it; and a light detector 3 detecting the return light from an organism tissue propagated through an insertion part 9 by the light transmitting member 18. The organism observation apparatus 1 is so formed that the tip face 9a of the insertion part 9 exposing the tip face 18a of the light transmitting member 18 has a form obliquely cut in the longitudinal direction and its distal end is provided with an acute part 19 capable of cutting the organism tissue.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供能够通过简单的结构观察生物体内的微观察目标部分的生物体观察装置。该生物体观察装置1具备光源2，柔性透光构件18，其传播来自光源2的光，将其从尖端面18a照射到观察对象部，接收从前端面18a的观察对象部分返回的返回光并使其传播; 以及光检测器3，其通过透光构件18检测从穿过插入部9传播的生物体组织的返回光。生物体观察装置1被形成为使得插入部分9的尖端面9a露出尖端面18a 透光构件18具有在纵向上倾斜切割的形状，并且其远端设置有能够切割有机体组织的锐角部19。版权所有（C）2006，JPO＆NCIPI

7. 发明专利

JP2010008565A Biological sample observation apparatus 审中-公开
标题翻译：生物样本观察装置
公开(公告)号：JP2010008565A
公开(公告)日：2010-01-14
申请号：JP2008165879
申请日：2008-06-25
申请人： Olympus Corp , オリンパス株式会社
发明人： NAGASAWA NOBUYUKI
IPC分类号： G02B21/18
摘要： PROBLEM TO BE SOLVED: To observe biological samples while switching a low magnification objective lens and a high magnification objective lens without moving a visual field.
SOLUTION: The biological sample observation apparatus 1 includes: a stage 7 for mounting the biological samples A; a casing 10; a wide visual field objective lens 4 fixed on the casing 10 to condense light from the biological samples A and a high resolution objective lens 8 having higher magnification than the wide visual field objective lens 4; a casing moving means 11 for moving the casing 10; observation optical systems 10, 12 for observing light condensed by the wide visual field objective lens 4 and the high resolution objective lens 8; and an optical path switching means 6 arranged between the wide visual field objective lens 4, the high resolution objective lens 8 and observation optical systems 10, 12 and alternatively switching an optical path of the wide visual field objective lens 4 or the high resolution objective lens 8. The wide visual field objective lens 4 includes a flexible transmission member 4a transmitting images of the biological samples A on the edge, and the edge part 4b of the transmission member 4a is fixed on the stage 7.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：在不移动视野的同时切换低倍率物镜和高倍率物镜时观察生物样品。生物样品观察装置1包括：用于安装生物样品A的载物台7; 壳体10; 固定在壳体10上的宽视场物镜4，以冷凝来自生物样品A的光和具有比宽视场物镜4高的倍率的高分辨率物镜8; 用于移动壳体10的壳体移动装置11; 用于观察由宽视场物镜4和高分辨率物镜8聚光的光的观察光学系统10,12; 以及布置在宽视场物镜4，高分辨率物镜8和观察光学系统10,12之间的光路切换装置6，并且交替地切换宽视场物镜4或高分辨率物镜的光路宽视场物镜4包括传递生物样品A在边缘上的图像的柔性透射部件4a，传播部件4a的边缘部分4b固定在载物台7上。版权所有（C））2010，JPO＆INPIT

8. 发明专利

JP2010066356A Microscope apparatus, controller of the same, and program 审中-公开
标题翻译： MICROSCOPE设备，其控制器和程序
公开(公告)号：JP2010066356A
公开(公告)日：2010-03-25
申请号：JP2008230629
申请日：2008-09-09
申请人： Olympus Corp , オリンパス株式会社
发明人： KOMURO TOMOYA , NAGASAWA NOBUYUKI
IPC分类号： G02B21/00 , G02B7/28 , G02B21/36
摘要： PROBLEM TO BE SOLVED: To appropriately observe a predetermined part even of a living body performing a dynamic behavior.
SOLUTION: An imaging element 21 picks up an optical image of an observation sample S obtained by image-forming observation light coming via an objective lens 3 from a focusing position of an objective lens 2. A focusing signal processing section 17 detects that a surface of the observation sample S is positioned at the focusing position of the objective lens 2 based on AF light that is emitted from a reference light source 3, is reflected on the surface, and comes via the objective lens 2. An offset lens group driving section 15 varies the focal distance of the objective lens 2 with the AF light. A control section 19 controls the offset lens group driving section 15 to make the focal distance different from the focal distance of the objective lens 2 with the observation light by a predetermined distance. An imaging trigger generating section 24 controls the imaging element 21 at the focal distance controlled by the control section 19 to pick up the optical image when the detection is performed by the focusing signal processing section 17.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：为了适当地观察甚至执行动态行为的生物体的预定部分。
解决方案：成像元件21拾取通过从物镜2的聚焦位置对来自物镜3的观察光进行成像而获得的观察样本S的光学图像。聚焦信号处理部17检测到观察样本S的表面基于从参考光源3发射的AF光位于物镜2的聚焦位置，在表面上被反射并通过物镜2进入。偏移透镜组驱动部分15利用AF灯改变物镜2的焦距。控制部19控制偏移透镜组驱动部15，使得与观察光的物镜2的焦距不同的焦距保持规定的距离。成像触发发生部分24控制由控制部分19控制的焦距处的成像元件21，以便在聚焦信号处理部分17执行检测时拾取光学图像。（C）2010， JPO＆INPIT

9. 发明专利

JP2009150829A Biological sample observation method 审中-公开
标题翻译：生物样本观察方法
公开(公告)号：JP2009150829A
公开(公告)日：2009-07-09
申请号：JP2007330423
申请日：2007-12-21
申请人： Olympus Corp , オリンパス株式会社
发明人： OBA MASAHIRO , NAGASAWA NOBUYUKI
IPC分类号： G01N21/76 , G01N21/64 , G01N21/78 , G01N33/48
CPC分类号： G06K9/20 , G06T5/50 , G06T7/0012 , G06T2207/20221 , H04N5/23232 , H04N5/2352
摘要： PROBLEM TO BE SOLVED: To observe biological samples of live cells, biological tissue, and small animals for a long time or a for a long period with quantitative performance maintained in the case of quantitatively observing such biological samples.
SOLUTION: An observation method for observing light emission or fluorescence emitted from biological samples is provided with the reference process S301 for making reference to image data acquired previous time; the luminance determination process S302 for determining whether the luminance of the image data acquired previous time is saturated or not; the exposure division process S303 for dividing the exposure to light of the image data, which has been previously acquired by one time, into a plurality of number of times in the case where it is determined in the luminance determination process that luminance is saturated; the image acquisition process S304 for acquiring images in the number of times of the plurality of number of times; and the integration process S306 for integrating the images acquired in the plurality of number of times into one image.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：长时间或长时间观察活细胞，生物组织和小动物的生物样品，在定量观察这些生物样品的情况下保持定量性能。解决方案：用于观察从生物样品发射的发光或荧光的观察方法具有参考处理S301，用于参考先前获得的图像数据; 用于确定先前获得的图像数据的亮度是否饱和的亮度确定处理S302; 在亮度饱和的亮度判定处理中确定的情况下，将先前已经取得一次的图像数据的曝光与曝光相分离的曝光分割处理S303成为多次; 用于获取多次次数的图像的图像获取处理S304; 以及用于将在多个次数中获取的图像集成为一个图像的积分处理S306。版权所有（C）2009，JPO＆INPIT

10. 发明专利

JP2006201610A Observing method and observation auxiliary device 有权
标题翻译：观察方法和观察辅助装置
公开(公告)号：JP2006201610A
公开(公告)日：2006-08-03
申请号：JP2005014625
申请日：2005-01-21
申请人： Olympus Corp , オリンパス株式会社
发明人： TANIGAWA YOSHIHISA , NAGASAWA NOBUYUKI
IPC分类号： G02B21/00
CPC分类号： G02B21/365 , G02B7/08 , G02B21/0012 , G02B21/244
摘要： PROBLEM TO BE SOLVED: To obtain an easy-to-see observation image by reducing blur occurring in an image without activating an observing optical system in real time in accordance with the movement of a specimen.
SOLUTION: In the observing method, an index member that is visible from outside is fixed on the surface of a specimen disposed within the observing range of a microscope, the surface of the specimen including the index member is imaged at predetermined time intervals, a plurality of captured images G are processed to calculate the movement locus Q of the index member, and observation is carried out while the optical axis of the observing optical system is fixed in the position where the movement locus Q of the index member in the observed position of the specimen is the shortest.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：通过减少图像中发生的模糊，而不是根据样本的移动实时地实时观察光学系统来获得易于观看的观察图像。解决方案：在观察方法中，将从外部可见的折射构件固定在设置在显微镜的观察范围内的试样的表面上，将包含折射构件的试样的表面以预定的时间间隔成像，对多个拍摄图像G进行处理，计算出折射构件的移动轨迹Q，观察光学系统的光轴被固定在指示构件的移动轨迹Q的位置样本的观察位置最短。版权所有（C）2006，JPO＆NCIPI

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