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1. 发明专利

JP2002296280A METHOD OF ANALYZING GENE EXPRESSION FREQUENCY USING CAPILLARY ARRAY 失效
公开(公告)号：JP2002296280A
公开(公告)日：2002-10-09
申请号：JP2001101700
申请日：2001-03-30
申请人： OLYMPUS OPTICAL CO
发明人： TAKAHASHI TAKEO
IPC分类号： G01N21/64 , C12M1/00 , C12M1/40 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N37/00
摘要： PROBLEM TO BE SOLVED: To provide a gene expression frequency analyzing method allowing precise and quantitative detection even in a small amount of a sample, excellent in reproducibility, affected hardly by a background, capable of conducting analysis by a simple detection equipment, and excellent in detection efficiency. SOLUTION: This gene expression frequency analyzing method is provided with (1) a process for solidifying plural kinds of targets having specific sequences in an inner wall of a capillary independently in the every kind, (2) a process for labeling a probe, (3) a process for coupling the labeled probe to the target in the capillary, (4) a process for disociating a nucleic acid coupled therein, (5) a process for conveying the dissociated nucleic acid by a liquid flow, and (6) a process for analyzing gene expression frequency having the specific sequence in order by detecting the labeled substance labeled hereinbefore.

2. 发明专利

JP2001275699A METHOD FOR ASSAYING REPETITIVE-SEQUENCE POLYMORPHISM 失效
公开(公告)号：JP2001275699A
公开(公告)日：2001-10-09
申请号：JP2000094264
申请日：2000-03-30
申请人： OLYMPUS OPTICAL CO
发明人： TAKAHASHI TAKEO
IPC分类号： G01N21/64 , C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method enabling the assay of repetitive-sequence polymorphism by convenient operations in a short time. SOLUTION: This method for assaying repetitive-sequence polymorphism comprises the following processes: (1) a process for collecting a target nucleic acid having a repetitive sequence from a subject; (2) a process for labeling the target nucleic acid; (3) a process for detecting the labeled target nucleic acid; and (4) a process for assaying the polymorphism of the repetitive sequence by analyzing the detected result by means of fluorescence correlation spectroscopy.

3. 发明专利

JPS61272660A IMMONOLOGICAL ANALYSIS USING BLOOD CELL 失效
公开(公告)号：JPS61272660A
公开(公告)日：1986-12-02
申请号：JP11496785
申请日：1985-05-28
申请人： OLYMPUS OPTICAL CO
发明人： YAMADA TAKASHI , KANEKO NOBUTAKA , TAWARA TAKASHI , TAKAHASHI TAKEO
IPC分类号： G01N33/555 , A61K39/00 , G01N33/543
摘要： PURPOSE:To prevent the disturbance of agglutination by the electrostatic repulsive force among the red cells and to make possible the exact immune reaction only by the measurement of the optical concn. of a reactive liquid by destructing the red cells fixed with an antigen on the surface, dispersing the same into the reactive liquid and causing the agglutination reaction. CONSTITUTION:Pure water is added to the suspension 2 of the red cells 1 fixed with the antigen on the surface to destruct the red cells, by which the suspension 5 of the blood cell membrane is obtd. The antibody 6 is added to the suspension 5 to form the agglutinated matter by the antigen-antibody reaction. The agglutinated matter settles in the bottom of a transparent reaction vessel 3 after the reaction and therefore light is irradiated from a light source 7 via a filter 8 to the vessel 3 from one side thereof. The photoelectric output of the light transmitted through the vessel 3 is detected by a photodetector 9 on the opposite side and the presence or absence of the agglutination is detected. The discrimination or the like of the blood group such as Rh blood group having the small blood agglutination power is thus easily exactly executed without the need for treating preliminarily the blood cell sample with bromelin (a kind of protein digestive enzyme) and without measuring the agglutination pattern.

4. 发明专利

JPS61271459A IDENTIFICATION OF BLOOD TYPE 失效
公开(公告)号：JPS61271459A
公开(公告)日：1986-12-01
申请号：JP11305585
申请日：1985-05-28
申请人： OLYMPUS OPTICAL CO
发明人： YAMADA TAKASHI , KANEKO NOBUTAKA , TAWARA TAKASHI , TAKAHASHI TAKEO
IPC分类号： G01N21/17 , A61K39/00 , G01N21/27 , G01N33/543 , G01N33/80
摘要： PURPOSE:To enable the identification of blood type in whole blood without any pretreatment, by immersing photoconductors which each have an anti-A antibody or -A antigen and an anti-B antibody or -B antigen converted to a solid phase on the end face thereof into a blood sample to optically measure the end face thereof. CONSTITUTION:An anti-A antibody 8 which will cause an antigen-antibody reaction specifically with A type blood cell in a blood sample and an anti-B antibody 9 which will cause an antigen-antibody reaction specifically with B type blood cell therein are arranged as converted to a solid phase on the end faces 4a and 5a of photoconductors 4 and 5. On the other hand, those arranged on the end face 6a of a reference photoconductor 6 are not converted to a solid phase. Then, a irradiation light from a lighting source 10 is made incident into the other end faces of the photoconductors 4-6 through a mirror 11. As the anti-A antibody and the anti-B antibody are converted to a solid phase respectively on the end faces 4a and 5a, blood cells are bonded to the respective end faces according to the blood types in the blood sample to be inspected. Hence, the irradiation light from the lighting source 10 is reflected on the surface of the blood cells to be incident into light receiving elements 12 and 13. As no blood cell is bonded to the end face 6a, it can be detected easily that the A type blood cell or the B type blood cell is bonded to the end face 4a or 5a by comparing the outputs of the light receiving elements 12 and 13 with the output of the light receiving element 14.

5. 发明专利

JPS61271455A IMMUNOLOGICAL ANALYSIS 失效
公开(公告)号：JPS61271455A
公开(公告)日：1986-12-01
申请号：JP11304985
申请日：1985-05-28
申请人： OLYMPUS OPTICAL CO
发明人： YAMADA TAKASHI , KANEKO NOBUTAKA , TAWARA TAKASHI , TAKAHASHI TAKEO
IPC分类号： G01N33/53 , A61K35/18 , A61K39/00 , G01N33/555
摘要： PURPOSE:To enable analysis of an incomplete antibody with an agglutination dispensing with any cleaning process while miniaturizing the equipment, by bonding complements together after activated by being acted on an immunological complex to perform an agglutination of blood cells. CONSTITUTION:A blood cell 2 having an antigen 1 which is allowed to react specifically with an incomplete antibody to be analyzed, complements (C1) 3 which will be activated by being bonded to an immunological complex through an antigen-antibody reaction between an antigen 1 and the incomplete antibody and an anti C1 antibody 4 which is used to bond the complements to be activated by being reacted therewith are arranged as reagents and these reagents are made to react with a serum sample. Then, when any incomplete antibody 5 to be analyzed exists in the sample, it bonds to the antigen 1 by an antigen- antibody reaction and the complements 3 are coupled to the resulting antigen- antibody complex to be activated and the complements thus activated get together through the anti C1 antibody 4 to agglutinate the blood cells 2. Thus, after the reaction between the reagents and the sample, the agglutination and non-agglutination are measured to identify the incomplete antibody 5.

6. 发明专利

JP2001275666A METHOD FOR CLONING cDNA FROM cDNA LIBRARY 失效
公开(公告)号：JP2001275666A
公开(公告)日：2001-10-09
申请号：JP2000098471
申请日：2000-03-31
申请人： OLYMPUS OPTICAL CO
发明人： TAKAHASHI TAKEO
IPC分类号： G01N33/543 , C12N1/00 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a method for isolating a microorganism containing a cDNA clone by binding a microorganism containing a cDNA clone to an insoluble carrier followed by collecting each of the microorganism-bound carriers in view of the fact that, if a cell of the microorganism is attached to a particle of the carrier, the size of the microorganism becomes larger, so that it becomes possible to isolate each of the cDNA clones and to make the isolated microorganism to grow. SOLUTION: This method for cloning a cDNA from a cDNA library comprises the steps of (a) contacting a population of a microorganism with a carrier to bind the microorganism to the carrier, (b) isolating a clone by dissociating each of the microorganisms from the microorganism-bound carriers, and (c) collecting a cDNA clone from the clone.

7. 发明专利

JPH09299087A ANALYSIS RELATED TO NUCLEIC ACID USING SCANNING TYPE PROBE MICROSCOPE 失效
公开(公告)号：JPH09299087A
公开(公告)日：1997-11-25
申请号：JP12183996
申请日：1996-05-16
申请人： OLYMPUS OPTICAL CO
发明人： TAKAHASHI TAKEO , OKADA TAKAO , HORI KUNIO , YAMAGUCHI MITSUSHIRO
IPC分类号： G01N33/50 , C07H21/02 , C07H21/04 , C12N15/09 , C12Q1/34 , C12Q1/68 , G06F17/30
摘要： PROBLEM TO BE SOLVED: To provide a method for carrying out the determination of a base sequence, mapping of a gene and analysis, etc., of exonuclease characteristics based on the high-order structure of a single-stranded nucleic acid and a linear shape of a double-stranded nucleic acid by directly observing an individual nucleic acid molecule itself such as a DNA and an RNA. SOLUTION: This method for determining a base sequence of a nucleic acid comprises (a) a step for constructing a database related to a high-order structure of a single-stranded nucleic acid and a base sequence corresponding to the high-order structure, (b) a step for preparing a nucleic acid for measurement in which at least a part expected to determine the base sequence is a single- stranded nucleic acid, (c) a step for measuring the nucleic acid for the measurement under a scanning type probe microscope and thereby providing a data for the high-order structure of the single-stranded part of the nucleic acid for measurement and (d) a step for comparing the data for the high-order structure of the nucleic acid for time measurement with the database and searching for the base sequence having the same high-order structure.

8. 发明专利

JPH0712816A BIO-SUBSTANCE MEASURING METHOD UTILIZING SPECIFIC BONDING REACTION 失效
公开(公告)号：JPH0712816A
公开(公告)日：1995-01-17
申请号：JP15718693
申请日：1993-06-28
申请人： OLYMPUS OPTICAL CO
发明人： NANBA AKIHIRO , TAKAHASHI TAKEO
IPC分类号： G01N33/543
摘要： PURPOSE:To determine an object to be measured with high sensitivity in a short time by determining the object based on the secular change of the detected intensity of light emission or fluorescence. CONSTITUTION:By catching the specific bonding reaction of a sample of a known concentration through an enzyme-substrate reaction, the detected intensity of generated luminescence (fluorescence) is continuously measured for a prescribed period of time. A characteristic curve prepared based on the measured results and indicating the relation between the measuring time and detected intensity, namely, the time characteristic is approximated to a straight line by using the method of least squares. The inclination of the straight line represents the secular change of the detected intensity and depends upon the quantity of a bio-substance contained in the sample. Therefore, by using a plurality of samples of known concentrations, a calibration curve representing the relation between the inclination of the time characteristic and concentration of a substance to be measured is prepared. Then, by receiving 12 the light emitted from a sample contained in a reaction container 11 and measuring 14 the inclination of the time characteristic of the sample containing the substance by an unknown amount, the substance to be measured in the sample is determined 15 from the calibration curve. Therefore, the substance can be determined without waiting for the saturation of detected intensity nor being affected by background noise caused by the stray light, etc., from the measuring instrument.

9. 发明专利

JPH06148075A CHEMILUMINESCENCE MEASURING INSTRUMENT 失效
公开(公告)号：JPH06148075A
公开(公告)日：1994-05-27
申请号：JP30193292
申请日：1992-11-12
申请人： OLYMPUS OPTICAL CO
发明人： NANBA AKIHIRO , TAKAHASHI TAKEO
IPC分类号： G01N21/76 , G01N33/543
摘要： PURPOSE:To provide a chemiluminescence measuring instrument which can perform solid-phase enzyme immunoassay by chemiluminescence in a short time even when the amount of the sample is small and, at the same time, can be easily operated. CONSTITUTION:The title measuring instrument is provided of a reaction container 11 composed of two rectangular plates 12 and 13, at least one of which has a light transmitting property and which are put upon another with two prismatic spacers 14 and 15 arranged in parallel with each other at the end sections of the plates 12 and 13 so that they can face each other in between and has a gap 16 which is formed between the plates 12 and 14 and into and from which a liquid can be injected and discharged by utilizing a capillary action, and a photomultiplier tube 21 with a photoelectric surface arranged in parallel with the surface of the plate 12 or 13 having the light transmitting property and constituting the container 11. The measuring instrument also has a low-noise amplifier 22 for amplifying signals from the tube 21, photo- counter 23 for counting signals from the amplifier 22, and microcomputer 24 for data-processing signals from the counter 23.

10. 发明专利

JPS61271458A IMMUNOLOGICAL ANALYSIS 失效
公开(公告)号：JPS61271458A
公开(公告)日：1986-12-01
申请号：JP11305285
申请日：1985-05-28
申请人： OLYMPUS OPTICAL CO
发明人： YAMADA TAKASHI , KANEKO NOBUTAKA , TAWARA TAKASHI , TAKAHASHI TAKEO
IPC分类号： G01N21/17 , A61K39/00 , G01N21/03 , G01N33/543
摘要： PURPOSE:To simplify the analysis process with a direct assay of a substance to be inspected in a sample, by arranging an antigen or an antibody allowed to cause an antigen-antibody reaction specifically with the substance being inspected on the end face of a photoconductor as converted to a solid phase to measure the state of the end face optically through the photoconductor after an immunological reaction. CONSTITUTION:An antigen or an antibody 3 allowed to cause an antigen-antibody reaction specifically with a substance to be inspected in a sample is arranged on one end face of a photoconductor 1 comprising a fiber band or the like is converted to a solid phase and the antigen or the antibody 3 is made to react with a blood sample 4 in a reactor 5 to couple a blood cell 6 as object to be inspected to the end face of the photoconductor 1. After the reaction, the reactor 5 is immersed into pure water 8 in a transparent container 7, the end face of the photoconductor 1 is irradiated with a lighting light from a lighting source 9 to detect the reflected light or the transmission light from the blood cell 6 coupled with a light receiving element 1 and then, the blood cell 6 coupled is assayed based on the detection output. As a result, the blood cell 6 in the blood sample 4 can be assayed directly to elevate the analysis accuracy along with a simplified analysis work.

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