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1. 发明专利

JP2008220179A New microorganism which degrade deoxynivalenol 有权
标题翻译：新型微生物降解脱氧维生素
公开(公告)号：JP2008220179A
公开(公告)日：2008-09-25
申请号：JP2007058335
申请日：2007-03-08
申请人： National Institute For Agro-Environmental Science , 独立行政法人農業環境技術研究所
发明人： TSUSHIMA SEIYA , KOITABASHI MOTOO , YOSHIDA SHIGENOBU , IKUNAGA YOKO
IPC分类号： C12N1/20 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To provide a new microorganism which can directly degrade deoxynivalenol, and to provide a method for degrading deoxynivalenol with the new microorganism. SOLUTION: A new microorganism RS1 strain (FERM AP-21224) belongs to alpha-proteobacteria and having an ability for degrading deoxynivalenol, and to a method for degrading deoxynivalenol, degrades the deoxynivalenol with the microorganism belonging to alpha-proteobacteria and having an ability for degrading the deoxynivalenol. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：要解决的问题：提供可以直接降解脱氧雪腐镰刀菌烯醇的新的微生物，并提供一种用新的微生物降解脱氧雪腐镰刀菌烯醇的方法。解决方案：新的微生物RS1菌株（FERM AP-21224）属于α-变形杆菌并具有降解脱氧雪腐镰刀菌烯醇的能力，并且降解脱氧雪腐镰刀菌烯醇的方法降解了属于α-变形菌的微生物的脱氧雪腐镰刀菌烯醇，并具有降解脱氧雪腐镰刀菌烯醇的能力。版权所有（C）2008，JPO＆INPIT

2. 发明专利

JP2011103863A Gene encoding protein having deoxynivalenol decomposing activity 审中-公开
标题翻译：具有脱氧硝基苯酚分解活性的基因编码蛋白质
公开(公告)号：JP2011103863A
公开(公告)日：2011-06-02
申请号：JP2009265762
申请日：2009-11-20
申请人： National Institute For Agro-Environmental Science , 独立行政法人農業環境技術研究所
发明人： TSUSHIMA SEIYA , ITO MICHIHIRO , KOITABASHI MOTOO
IPC分类号： C12N15/09 , C12N1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00
摘要： PROBLEM TO BE SOLVED: To provide a protein having deoxynivalenol decomposing activity and a gene encoding the protein. SOLUTION: There are disclosed: (A) an amino acid sequence comprising a specific sequence; (B) an amino acid sequence in which one or more amino acids in the amino acid sequence (A) comprising a specific sequence are deleted, substituted or added and having deoxynivalenol decomposing activity; or (C) an amino acid sequence having at least 80% or more homology to the amino acid sequence (A) comprising a specific sequence and deoxynivalenol decomposing activity. Further there are disclosed: (D) a base sequence comprising a specific sequence different from (A); or (E) a base sequence in which one or more bases in the base sequence (D) are deleted, substituted or added. These are base sequences encoding a protein having deoxynivalenol decomposing activity. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供具有脱氧雪腐镰刀菌烯醇分解活性的蛋白质和编码该蛋白质的基因。解决方案：公开了：（A）包含特定序列的氨基酸序列; （B）氨基酸序列，其中包含特定序列的氨基酸序列（A）中的一个或多个氨基酸被缺失，取代或添加并具有脱氧雪腐镰刀菌烯醇分解活性; 或（C）与氨基酸序列（A）具有至少80％以上同源性的氨基酸序列，其包含特定序列和脱氧雪腐镰刀菌烯醇分解活性。此外，还公开了：（D）包含与（A）不同的特定序列的碱基序列; 或（E）其中碱基序列（D）中的一个或多个碱基被缺失，取代或加入的碱基序列。这些是编码具有脱氧雪腐镰刀菌烯醇分解活性的蛋白质的碱基序列。版权所有（C）2011，JPO＆INPIT

3. 发明专利

JP2008237212A Biodegradable plastic-decomposing bacteria and method for producing decomposing enzyme 有权
标题翻译：生物可降解塑料分解细菌和生产分解酶的方法
公开(公告)号：JP2008237212A
公开(公告)日：2008-10-09
申请号：JP2008023030
申请日：2008-02-01
申请人： National Institute For Agro-Environmental Science , 独立行政法人農業環境技術研究所
发明人： KITAMOTO HIROKO , KOITABASHI MOTOO , TSUSHIMA SEIYA , SAKAMOTO HIRONORI
IPC分类号： C12N15/09 , C07K14/39 , C12N9/16
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a new biodegradable plastic-decomposing enzyme. SOLUTION: This method for producing the biodegradable plastic-decomposing enzyme comprises the following processes. (1) A process of preparing samples for screening, collected from yeasts, filamentous fungi or bacteria living on the surface of a plant leaf, (2) a process of screening the yeasts, filamentous fungi or bacteria decomposing the biodegradable plastic, (3) a process of culturing the screened yeasts, filamentous fungi or bacteria in a medium containing the biodegradable plastic for secreting the biodegradable plastic-decomposing enzyme in the culturing liquid, and (4) a process of purifying the biodegradable plastic-decomposing enzyme from the culturing liquid. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：要解决的问题：提供一种生产可生物降解的塑料分解酶的方法。解决方案：该生物降解塑料分解酶的制造方法包括以下工序。（1）从生长在植物叶表面的酵母，丝状真菌或细菌收集的筛选样品的制备方法，（2）筛选分解生物降解性塑料的酵母，丝状真菌或细菌的方法，（3）在含有生物降解性塑料的培养液中培养筛选的酵母，丝状真菌或细菌的方法，用于分泌培养液中的生物降解性塑料分解酶，以及（4）从培养液中纯化生物降解塑料分解酶的方法。版权所有（C）2009，JPO＆INPIT

4. 发明专利

JP2013023643A Method for accelerating decomposition of biodegradable plastic material 审中-公开
标题翻译：用于加速生物可降解塑料材料分解的方法
公开(公告)号：JP2013023643A
公开(公告)日：2013-02-04
申请号：JP2011161763
申请日：2011-07-25
申请人： National Institute For Agro-Environmental Science , 独立行政法人農業環境技術研究所
发明人： KOITABASHI MOTOO , KITAMOTO HIROKO , NAKAZAWA YUKIHIRO , FUJII TAKESHI , WATABE TAKASHI , TSUSHIMA SEIYA
IPC分类号： C08J11/18 , B01J20/22 , B01J20/24 , B01J20/26 , C08L101/00 , C08L101/14 , C08L101/16 , C12N9/00
CPC分类号： Y02W30/706
摘要： PROBLEM TO BE SOLVED: To provide a method for decomposing a biodegradable plastic material and a biodegradable plastic material decomposing agent, permitting decomposing the biodegradable plastic material efficiently.SOLUTION: Since in the method for decomposing the biodegradable plastic material including a step of applying a biodegradable plastic decomposing enzyme and a polymerized water-absorptive agent to the surface of the biodegradable plastic material, the polymeric desiccant is used as the water-absorptive agent, efficient decomposition of the biodegradable plastics material can be achieved for a long period of time.
摘要翻译：要解决的问题：提供一种分解生物可降解塑料材料和可生物降解的塑料材料分解剂的方法，可有效地分解可生物降解的塑料材料。解决方案：由于在生物可降解塑料材料的分解方法中，包括将可生物降解塑料分解酶和聚合吸水剂施加到可生物降解塑料材料表面的步骤，因此使用聚合物干燥剂作为水溶性聚合物，吸收剂，可以长时间实现生物降解塑料材料的有效分解。版权所有（C）2013，JPO＆INPIT

5. 发明专利

JP2011103864A Gene encoding protein having deoxynivalenol and nivalenol decomposing activity 审中-公开
标题翻译：具有脱氧十氮烯醇和NIVALENOL分解活性的基因编码蛋白
公开(公告)号：JP2011103864A
公开(公告)日：2011-06-02
申请号：JP2009265763
申请日：2009-11-20
申请人： National Institute For Agro-Environmental Science , 独立行政法人農業環境技術研究所
发明人： TSUSHIMA SEIYA , ITO MICHIHIRO , KOITABASHI MOTOO
IPC分类号： C12N15/09 , C12N1/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00
摘要： PROBLEM TO BE SOLVED: To provide a P450 protein having deoxynivalenol and nivalenol decomposing activity and a gene encoding the protein. SOLUTION: There are disclosed: a deoxynivalenol and/or nivalenol decomposing enzyme protein having an amino acid sequence comprising a specific amino acid sequence or an amino acid sequence in which one or more amino acids in the specific amino acid sequence are deleted, substituted or added and encoding an amino acid sequence having deoxynivalenol and/or nivalenol decomposing activity; and a deoxynivalenol and/or nivalenol decomposing enzyme gene including a specific base sequence or a base sequence in which one or more bases in the specific base sequence are deleted, substituted or added. COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供具有脱氧雪腐镰刀菌烯醇和新戊烯醇分解活性的P450蛋白质和编码该蛋白质的基因。解决方案：公开了具有氨基酸序列的脱氧雪腐镰刀菌烯醇和/或异戊烯醇分解酶蛋白，所述氨基酸序列包含缺失特定氨基酸序列中的一个或多个氨基酸的特定氨基酸序列或氨基酸序列，取代或添加并编码具有脱氧雪腐镰刀菌烯醇和/或新戊烯醇分解活性的氨基酸序列; 以及包含特定碱基序列或碱基序列的脱氧雪腐镰刀菌烯醇和/或新戊烯醇分解酶基因，其中特定碱基序列中的一个或多个碱基被缺失，取代或添加。版权所有（C）2011，JPO＆INPIT

6. 发明专利

JP2014129287A Herbicidal composition comprising enzyme and extermination method of adverse plant 有权
标题翻译：包含酶的除草组合物和不良植物的外推方法
公开(公告)号：JP2014129287A
公开(公告)日：2014-07-10
申请号：JP2012288082
申请日：2012-12-28
申请人： National Institute For Agro-Environmental Science , 独立行政法人農業環境技術研究所
发明人： KITAMOTO HIROKO , KUGIMIYA SOICHI , UEDA KOICHI , KUROSE DAISUKE , YOSHIDA SHIGENOBU , TABATA JUN , KOITABASHI MOTOO , WATABE TAKASHI , SHINOZAKI YUKIKO
IPC分类号： A01N63/00 , A01M21/00 , A01P13/00
摘要： PROBLEM TO BE SOLVED: To provide a herbicidal composition efficiently and effectively used for extermination of an adverse plant, and to provide an extermination method of an adverse plant.SOLUTION: A herbicidal composition comprising an enzyme is used for extermination of an adverse plant and has hydrolysis activity of p-Nitrophenyl butyrate. The composition comprises: a cutinase-like enzyme having no pathogenicity against a plant and derived from a microorganism; and a plant pathogenic microorganism being a herbicidal pesticide. The content of the cutinase-like enzyme in the herbicidal composition comprising the enzyme is 0.01 U/mL or more in terms of the hydrolysis activity of p-Nitrophenyl butyrate.
摘要翻译：要解决的问题：提供有效地有效地用于灭菌不利植物的除草组合物，并提供不利植物的灭绝方法。解决方案：包含酶的除草组合物用于灭绝不利植物，并且具有丁酸对硝基苯酯的水解活性。该组合物包含：对植物没有致病性并衍生自微生物的角质酶样酶; 以及作为除草农药的植物病原微生物。含有该酶的除草组合物中的角质酶样酶的含量就丁酸对硝基苯酯的水解活性而言为0.01U / mL以上。

7. 发明专利

JP2013048563A Method for producing biodegradable plastic-degrading enzyme and pseudozyma antarctica for use in the same 有权
标题翻译：生产可降解塑料降解酶和PSEUDOZYMA ANTARCTICA的方法，用于其中
公开(公告)号：JP2013048563A
公开(公告)日：2013-03-14
申请号：JP2011186786
申请日：2011-08-30
申请人： National Institute For Agro-Environmental Science , 独立行政法人農業環境技術研究所
发明人： KITAMOTO HIROKO , KOITABASHI MOTOO , YOSHIDA SHIGENOBU , WATABE TAKASHI
IPC分类号： C12N9/24 , C12N1/20 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To develop a technique of mass-producing a biodegradable plastic-degrading enzyme using a microorganism which is isolated from nature and in which the safety thereof is confirmed, without using genetic recombination technology.SOLUTION: In the method for producing the biodegradable plastic-degrading enzyme, Pseudozyma antarctica is cultured in a medium containing xylose. With the method, the biodegradable plastic-degrading enzyme can be mass-produced without using the genetic recombination technology. Thus, the mass-produced biodegradable plastic-degrading enzyme can be used for decomposing a biodegradable multi film laid in an agricultural field and the biodegradable mulch film can be safely and efficiently decomposed.
摘要翻译：要解决的问题：开发使用从自然界分离并且确保其安全性的微生物大规模生产可生物降解的塑料降解酶的技术，而不使用遗传重组技术。解决方案：在生产可降解塑料降解酶的方法中，将南极假丝酵母在含有木糖的培养基中培养。通过该方法，生物降解塑料降解酶可以在不使用遗传重组技术的情况下批量生产。因此，大量生产的可降解塑料降解酶可用于分解农业领域中生物降解的多层膜，并且可生物降解的覆盖膜可以安全有效地分解。版权所有（C）2013，JPO＆INPIT

8. 发明专利

JP2011103862A Novel microorganism decomposing deoxynivalenol and nivalenol 审中-公开
标题翻译：新型微生物分解脱氧维生素和尼维那醇
公开(公告)号：JP2011103862A
公开(公告)日：2011-06-02
申请号：JP2009265761
申请日：2009-11-20
申请人： National Institute For Agro-Environmental Science , 独立行政法人農業環境技術研究所
发明人： TSUSHIMA SEIYA , ITO MICHIHIRO , KOITABASHI MOTOO
IPC分类号： C12N1/20 , C12N1/00 , C12N15/09
摘要： PROBLEM TO BE SOLVED: To provide a novel microorganism decomposing deoxynivalenol and nivarenol.
SOLUTION: There are disclosed: a microorganism belonging to alpha-proteobacteria and having deoxynivalenol and nivalenol decomposing ability and 98% or more homology in the base sequence of 16S rRNA gene of a novel microorganism KSM1 strain; and a novel microorganism KSM1 strain belonging to alpha-proteobacteria and having deoxynivalenol and nivalenol decomposing ability. Furthermore, there are disclosed: a method for decomposing deoxynivalenol and/or nivalenol using the microorganism; and a deoxynivalenol and/or nivalenol decomposing agent which includes the microorganism.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供一种新型的微生物分解脱氧雪腐镰刀菌烯醇和尼可卡因。解决方案：公开了一种属于α-变形细菌的微生物，具有脱氧雪腐镰刀菌烯醇和新戊烯醇分解能力，并且在新型微生物KSM1菌株的16S rRNA基因的碱基序列中具有98％以上的同源性; 和属于α-变形细菌的新型微生物KSM1菌株，具有脱氧雪腐镰刀菌烯醇和新戊烯醇分解能力。此外，公开了使用微生物分解脱氧雪腐镰刀菌烯醇和/或新戊烯醇的方法; 以及包含微生物的脱氧雪腐镰刀菌烯醇和/或新戊烯醇分解剂。版权所有（C）2011，JPO＆INPIT

9. 发明专利

JP2010227006A Activity inhibitor of biodegradable plastic decomposing enzyme 有权
标题翻译：生物可降解塑料分解酶的活性抑制剂
公开(公告)号：JP2010227006A
公开(公告)日：2010-10-14
申请号：JP2009078020
申请日：2009-03-27
申请人： National Institute For Agro-Environmental Science , National Institute Of Advanced Industrial Science & Technology , 独立行政法人産業技術総合研究所 , 独立行政法人農業環境技術研究所
发明人： KITAMOTO HIROKO , TSUSHIMA SEIYA , KOITABASHI MOTOO , FUKUOKA TOKUMA , MORITA TOMOTAKE , IMURA TOMOHIRO , KITAMOTO MASARU
IPC分类号： C12N9/99 , C08G63/02 , C08L101/16 , C12N9/16
摘要： PROBLEM TO BE SOLVED: To provide a technique which controls decomposition of biodegradable plastic, thereby enabling to better utilize the biodegradable plastic.
SOLUTION: This activity inhibitor of biodegradable plastic decomposing enzyme contains a nonionic surfactant at concentration higher than critical micelle concentration.
COPYRIGHT: (C)2011,JPO&INPIT

10. 发明专利

JP2013127027A Ultraviolet absorber composition originated from plant inhabiting microbes 有权
标题翻译：来自植物吸收微生物的超紫外线吸收剂组合物
公开(公告)号：JP2013127027A
公开(公告)日：2013-06-27
申请号：JP2011276731
申请日：2011-12-19
申请人： National Institute For Agro-Environmental Science , 独立行政法人農業環境技術研究所
发明人： YOSHIDA SHIGENOBU , TSUSHIMA SEIYA , HIRADATE TOSHITARO , KOITABASHI MOTOO
IPC分类号： C09K3/00 , A61K8/99 , A61Q17/04 , C12N1/20 , C12P1/00 , C12R1/01
摘要： PROBLEM TO BE SOLVED: To provide a new ultraviolet absorber composition originated from a natural component.SOLUTION: The ultraviolet absorber composition contains a component originating from plant inhabiting microbes, and has an absorption peak of ultraviolet absorption spectrum in a wavelength region of 320-400 nm. From microbes inhabiting the phyllosphere exposed to various environmental stresses such as ultraviolet ray, an ultraviolet absorption component having an absorption effect, UV-A in particular, can be obtained. A cosmetic contains the composition with the ultraviolet absorption component. Also, there is provided a method for producing the ultraviolet absorber composition including the steps of culturing the microbes containing the component, extracting a solvent and separating the microbes from the solvent.
摘要翻译：要解决的问题：提供源自天然成分的新的紫外线吸收剂组合物。解决方案：紫外线吸收剂组合物含有源自植物微生物的成分，并且在320-400nm的波长范围内具有紫外吸收光谱的吸收峰。从暴露于紫外线等各种环境应力的叶片的微生物中，可以获得具有吸收效果的紫外线吸收成分，特别是UV-A。化妆品含有具有紫外线吸收成分的组合物。另外，提供了一种紫外线吸收剂组合物的制造方法，其特征在于，包括培养含有成分的微生物，萃取溶剂，从溶剂中分离微生物的工序。版权所有（C）2013，JPO＆INPIT

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