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    • 2. 发明专利
    • PRODUCTION OF MICROORGANISMIC PRODUCT
    • JPH0376586A
    • 1991-04-02
    • JP21319589
    • 1989-08-18
    • NGK INSULATORS LTD
    • KAMIYA YOSHIHIROIMAIZUMI YASUSHI
    • C12P1/00C12M1/00C12P7/06
    • PURPOSE:To accomplish production of subject materials in high efficiency using microorganisms in such a way that a fermented liquid in the foregoing stage, removed from a specific sprinkling filter bed-type bioreactor as fermentation tank is converted into a product in a latter stage fermentation tank, and the resulting fermented liquid is then separated. CONSTITUTION:The substrate concentration in a fermentation tank 1 is set at fairly higher level to ensure the substrate concentration not to be a rate- determining step. The liquid in the tank 1 is a mixed one comprising the substrate, the product and the microorganism isolated from a carrier and a part of the fermented liquid in the foregoing stage is circulated again to the tank 1 to prevent the reduction of the microorganismic concentration in the tank. The former stage-fermented liquid removed from the tank 1 is then transferred via a feed pipe 16 to a latter fermentation tank 11. The latter stage-fermented liquid removed from the tank 11 is separated by using a membrane separator 19 into a clear solution with low microorganismic concentration and high product concentration and the remaining concentrate with high microorganismic concentration.
    • 8. 发明专利
    • PRODUCTION OF MICROORGANISMIC PRODUCT MICROORGANISM
    • JPH0376585A
    • 1991-04-02
    • JP21319489
    • 1989-08-18
    • NGK INSULATORS LTD
    • KAMIYA YOSHIHIROIMAIZUMI YASUSHI
    • C12P1/00C12M1/00
    • PURPOSE:To accomplish production of the subject material in high efficiency using microorganisms in such a way that a liquid removed from a fermentation tank with specified setup is separated through a membrane separator and a clear solution of high product concentration is removed externally, the rest being returned to the fermentation tank. CONSTITUTION:When a fermentation tank 1 is charged with a substrate such as an aqueous solution containing ca.20wt.% of glucose, ethanol etc., is produced by the action of microorganisms immobilized on a relevant carrier in the tank 1. A fermentation liquid comprising the resultant useful materials and the substrate is allowed to flow down to the bottom of the tank 1, put to suction through a pump 5, and then sprayed again via a nozzle 3 installed on the upper part of a packed tank 2 into the fermentation tank 1. Thus, the concentration of the product is gradually increased during repeating such circulation, and a part thereof is removed from an outlet for the fermentation liquid. With the present method, the fermentation liquid is allowed to flow down uniformly, therefore causing no cloggings.
    • 9. 发明专利
    • CARRIER CONTAINING IMMOBILIZED MICROORGANISM
    • JPS63202384A
    • 1988-08-22
    • JP3425787
    • 1987-02-17
    • NGK INSULATORS LTD
    • KAMIYA YOSHIHIROKAWASE MITSUOMAJIMA TAKESHI
    • C12N11/14
    • PURPOSE:To increase the amount of immobilized microorganisms per unit area of a carrier, shorten treating time and improve treatment efficiency, by carrying and immobilizing a microorganism on the surface of a ceramic carrier having surface electric potential of opposite polarity to that of the surface potential of the microorganism. CONSTITUTION:The surface potential of a microorganism and that of a ceramic carrier are premeasured. The surface potential of the microorganism is measured by suspending the microbial cells in, e.g. a test solution adjusted to pH3 and 0.3mS electric conductivity with HCl and KCl, injecting the suspension into Brigg's cell having electrodes mounted at both ends, measuring migration velocity of the microorganism under a microscope while passing a current across both electrodes and judging the positive or negative electric charge from the moving direction at the same time. The surface potential of the ceramic carrier is similarly measured by finely pulverizing the carrier. The microorganism is then carried and immobilized on the surface of the ceramic carrier having the surface potential of polarity opposite to that thereof.