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    • 3. 发明专利
    • Substrate plate for detecting oceanic microorganism and method for monitoring ocean
    • 用于检测海洋微生物的基板和监测海洋的方法
    • JP2005176791A
    • 2005-07-07
    • JP2003425757
    • 2003-12-22
    • Mitsubishi Heavy Ind Ltd三菱重工業株式会社
    • SUGIMORI MIHOTAKEUCHI KAZUHISA
    • G01N33/569C12M1/00C12N15/09C12Q1/68G01N37/00
    • PROBLEM TO BE SOLVED: To provide a method for monitoring an ocean, capable of grasping the state of the ocean quickly, and analyzing tools used for the same, or the like.
      SOLUTION: The method for monitoring the ocean comprises collecting sea water from a prescribed sea zone to prepare a specimen, extracting polynucleotides of microorganisms contained in the specimen, testing polynucleotides 5 extracted from the specimen with a substrate plate 1 immobilized a polynucreotide 3 derived from at least 1 kind of a microorganism belonging to any of prescribed bacterial groups such as an α-proteobacteria class and used for detecting the oceanic microorganisms, detecting whether or not the polynucleotide 5 extracted from the specimen hybridizes with the polynucleotide immobilized on the substrate plate for detecting the ocean microorganisms, and whether or not the bacterial species or Archaeal species from which the polynucleotide immobilized on the substrate plate for detecting the oceanic microorganisms, is present in the sea water and analyzing the detected testing results by using the substrate plate for detecting the oceanic microorganisms.
      COPYRIGHT: (C)2005,JPO&NCIPI
    • 要解决的问题:提供一种能够快速掌握海洋状态的海洋监测方法,以及分析其使用的工具等。 解决方案:用于监测海洋的方法包括从规定的海域收集海水以制备样品,提取样品中包含的微生物的多核苷酸,测试从样品中提取的多核苷酸5,固定有多柔比星3 衍生自属于任何规定细菌群体的α-微生物类别的至少1种微生物,用于检测海洋微生物,检测从标本中提取的多核苷酸5是否与固定在基质上的多核苷酸杂交 用于检测海洋微生物的板,以及固定在基板上用于检测海洋微生物的多核苷酸的细菌种类或古菌种是否存在于海水中,并且通过使用基板将其检测出的测试结果分析 检测海洋微生物。 版权所有(C)2005,JPO&NCIPI
    • 5. 发明专利
    • Method for analyzing microorganism community
    • 分析微生物群落社区的方法
    • JP2005065605A
    • 2005-03-17
    • JP2003300624
    • 2003-08-25
    • Japan Science & Technology AgencyMitsubishi Heavy Ind Ltd三菱重工業株式会社独立行政法人科学技術振興機構
    • SUGIMORI MIHOTAKEUCHI KAZUHISAEDOWAADO BARAN
    • A01K61/00C02F3/34C12N15/09C12Q1/04C12Q1/68G01N30/88
    • Y02A40/81Y02A40/814
    • PROBLEM TO BE SOLVED: To provide a method for analyzing a microorganism community by which the state of the microorganism community in the ecological system can easily be grasped. SOLUTION: The state of the microorganism community is gasped by collecting microorganisms present in a test object area, extracting nucleic acids from the microorganisms, amplifying a prescribed sites of the extracted nucleic acids, subjecting the amplified nucleic acids to a separation treatment by using a modified high-performance liquid chromatography, and analyzing the results of the separation as the test object area. A region encoding 16S rRNA, or the like is preferable as the prescribed site of the nucleic acid to be amplified. The detection of a causative bacterium of pollution in the ecological system, the evaluation of the relation between the concentration of CO 2 in sea water and the microorganism community, the detection of a causative bacterium of decay, or the like is carried out as a more concrete example. COPYRIGHT: (C)2005,JPO&NCIPI
    • 要解决的问题:提供一种用于分析微生物群落的方法,通过该方法可以容易地掌握生态系统中微生物群落的状态。 解决方案:通过收集在试验对象区域中存在的微生物,从微生物中提取核酸,扩增所提取的核酸的规定位点,对扩增的核酸进行分离处理,使微生物群落的状态 使用改进的高效液相色谱法,并将分离结果作为测试对象区域进行分析。 编码16S rRNA等的区域优选作为待扩增核酸的规定位点。 在生态系统中检测污染致病菌,评估海水中微生物群落中CO 2 浓度与腐烂致病菌的检测关系, 像一个更具体的例子。 版权所有(C)2005,JPO&NCIPI
    • 6. 发明专利
    • Position holding system for submarine structure
    • 位置控制系统用于海底结构
    • JP2003081188A
    • 2003-03-19
    • JP2001276558
    • 2001-09-12
    • Mitsubishi Heavy Ind Ltd三菱重工業株式会社
    • MIURA MASAMIKONO TAKAYUKIMURAKAMI CHIKASUKESUGIMORI MIHOSETTA KOJINAKAHAMA TAKESHI
    • A01K61/00B63B35/44B63G8/08B63H21/18B63H25/42
    • Y02A40/81Y02A40/84Y02T70/5227
    • PROBLEM TO BE SOLVED: To improve the factors of worsening global environment by recycling waste, effluents including drainage or excretion for reducing the amount of unilateral waste and the exhaust amount of carbon dioxide. SOLUTION: The effluents are used as raw materials to produce nutrient salts, an ocean ecological system in an ocean is established based on the nutrient salts, fishery resources produced in the process of circulating the ocean ecological system are gathered, effluents discharged after consuming the fishery resources are collected and the effluents are used as raw materials to reproduced nutrient salts, thus permitting the recycling the fishery resources. In particular, submarine structures formed by simulating a sea bottom are suspended in an ocean, environment suitable for aquatic organisms including fishes and shellfishes and aquatic plants as fishery resources to inhabit in the submarine structures 2 is constructed and thrust acting against an ocean current is given to make the submarine structures 2 stay in position.
    • 要解决的问题:通过回收废物,包括排放或排泄的废水,以减少单边废物的量和二氧化碳的排放量来改善全球环境恶化的因素。 解决方案:将废水作为生产营养盐的原料,以营养盐为基础建立海洋中的海洋生态系统,在海洋生态系统循环过程中产生的渔业资源得到收集,渔业消费后排放出来 收集资源,将废水用作再生营养盐的原料,从而可以回收渔业资源。 特别是通过模拟海底形成的海底结构悬浮在海洋中,适用于包括鱼类和贝类和水生植物在内的水生生物的环境,作为在海底结构2中居住的渔业资源被构造,并且对海洋电流施加推力 使潜艇结构2保持在适当位置。
    • 7. 发明专利
    • Method for determining base sequence or method for quantitatively measuring nucleic acid by using dna chip
    • 用于确定基因序列的方法或使用DNA芯片定量测量核酸的方法
    • JP2003000300A
    • 2003-01-07
    • JP2001184949
    • 2001-06-19
    • Mitsubishi Heavy Ind Ltd三菱重工業株式会社
    • TAKEUCHI KAZUHISASUGIMORI MIHO
    • G01N33/53C12M1/00C12M1/38C12M1/40C12N15/09C12Q1/68G01N33/566
    • PROBLEM TO BE SOLVED: To provide a method by which hybridization is carried out with high quantification at once by using a DNA chip, and further to provide an apparatus for carrying out the method.
      SOLUTION: The method for determining a base sequence or that for quantitatively measuring a nucleic acid, use a DNA chip having plural number of base sequences as a same sequence immobilized thereon, and arranged in one row. The method by using the DNA chip is characterized in that the method comprises forming a temperature gradient in the direction of the immobilized same base sequences, subjecting the same base sequence to be detected to the hybridization on the DNA chip after labeling the each base sequence to be detected, washing the resultant chip by a buffer solution, and determining the base sequence to be detected by comparing the intensities of the label of each base sequence immobilized in the row. The device for the method for determining the base sequence or quantitatively measuring the nucleic acid is characterized in that the device has a buffer solution-storing container, a means for heating the buffer solution, a passage for flowing the buffer solution on the DNA chip, and a means for measuring the intensity of the label.
      COPYRIGHT: (C)2003,JPO
    • 要解决的问题:提供通过使用DNA芯片一次以高定量进行杂交的方法,并且还提供用于实施该方法的装置。 解决方案:用于确定碱基序列或用于定量测量核酸的方法,使用具有多个碱基序列的DNA芯片作为固定在其上的相同序列,并排列成一行。 通过使用DNA芯片的方法的特征在于,该方法包括在固定的相同碱基序列的方向上形成温度梯度,在将每个碱基序列标记之后,对与DNA芯片上的杂交进行相同的碱基序列进行检测 通过缓冲溶液洗涤所得到的芯片,并且通过比较固定在该行中的每个碱基序列的标签的强度来确定要检测的碱基序列。 用于确定碱基序列或定量测量核酸的方法的装置的特征在于该装置具有缓冲溶液储存容器,用于加热缓冲溶液的装置,将缓冲溶液流过DNA芯片的通道, 以及用于测量标签强度的方法。
    • 8. 发明专利
    • Underwater construction
    • 水下施工
    • JP2003079267A
    • 2003-03-18
    • JP2001276557
    • 2001-09-12
    • Mitsubishi Heavy Ind Ltd三菱重工業株式会社
    • MIURA MASAMIKONO TAKAYUKIMURAKAMI CHIKASUKESUGIMORI MIHOSETTA KOJINAKAHAMA TAKESHI
    • A01K61/00E02B3/04
    • Y02A40/81Y02A40/834Y02A40/838
    • PROBLEM TO BE SOLVED: To improve various factors to destroy the global environment by reusing waste materials such as domestic wastes, domestic sewage and excrements to reduce the discharged amount of the waste and the generation of carbon dioxide.
      SOLUTION: Nutrient salts are produced from excrements as a raw material and a marine ecosystem is established at a distant water area with the nutrient salts. Aquatic resources produced by the circulation in the marine ecosystem is collected, the excrements produced by the consumption of the aquatic resource are recovered and the nutrient salts are produced from the excrement to recycle the aquatic resources. Especially, a structure 10 having a step-wise upper face is placed in the sea at a distant water area and a sea bottom forming a marine ecosystem is simulated by the structure 10.
      COPYRIGHT: (C)2003,JPO
    • 要解决的问题:通过重复使用废物,生活污水和排泄物等废物来改善各种因素来破坏全球环境,减少废物的排放量和二氧化碳的产生。 解决方案:营养盐由作为原料的排泄物生产,海洋生态系统建立在具有营养盐的遥远的水域。 收集海洋生态系统循环产生的水生资源,回收水产资源消耗产生的排泄物,营养盐从排泄物中回收利用水生资源。 特别地,具有逐步上表面的结构10被放置在远离水域的海中,并且由结构10模拟形成海洋生态系统的海底。