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1. 发明专利

JP2014100095A Fusion protein for protein detection and method for protein detection 有权
标题翻译：用于蛋白质检测的融合蛋白质和蛋白质检测方法
公开(公告)号：JP2014100095A
公开(公告)日：2014-06-05
申请号：JP2012254116
申请日：2012-11-20
申请人： Kyushu Univ , 国立大学法人九州大学 , Hitachi Aloka Medical Ltd , 日立アロカメディカル株式会社
发明人： KAMIYA TENHO , TOMOZOE YUSUKE , MATSUBA KYOICHI , HAYASHI KONOSUKE , NAGAI KENJI
IPC分类号： C12N15/09 , C07K14/315 , C07K19/00 , C12N9/16 , C12Q1/42 , G01N33/535 , G01N33/566
摘要： PROBLEM TO BE SOLVED: To provide a fusion protein for protein detection having high versatility, high detection sensitivity and high stability.SOLUTION: Provided is a fusion protein for protein detection being a fusion between a protein domain and a fusion alkaline phosphatase, where the protein domain includes at least one of protein G C2 domain and protein G C3 domain, and the fusion alkaline phosphatase is a fusion between partial sequences of human placenta alkaline phosphatase and human intestine alkaline phosphatase.
摘要翻译：要解决的问题：提供具有高通用性，高检测灵敏度和高稳定性的蛋白质检测用融合蛋白。解决方案：蛋白质检测的融合蛋白是蛋白质结构域和融合碱性磷酸酶之间的融合蛋白，其中蛋白质结构域包括蛋白G C2结构域和蛋白G C3结构域中的至少一个，融合碱性磷酸酶是人胎盘碱性磷酸酶和人肠碱性磷酸酶的部分序列之间的融合物。

2. 发明专利

JP2014156419A Fusion protein, her2 protein detection probe, method of producing her2 protein detection probe and method of detecting her2 protein 有权
标题翻译：融合蛋白，HER2蛋白质检测探针，产生HER2蛋白质检测探针的方法和检测HER2蛋白的方法
公开(公告)号：JP2014156419A
公开(公告)日：2014-08-28
申请号：JP2013027632
申请日：2013-02-15
申请人： Kyushu Univ , 国立大学法人九州大学 , Hitachi Aloka Medical Ltd , 日立アロカメディカル株式会社
发明人： KAMIYA TENHO , TADA YUTAKA , HAYASHI KONOSUKE
IPC分类号： C07K19/00 , C07K14/31 , C12N9/16 , C12N15/09 , G01N33/53 , G01N33/535
摘要： PROBLEM TO BE SOLVED: To provide a fusion protein which can detect HER2 protein with excellent sensitivity, and is applicable to the production of a HER2 protein detection probe.SOLUTION: This present invention relates to a fusion protein in which a protein A mutant capable of binding to HER 2 protein is fused to a marker enzyme.
摘要翻译：要解决的问题：提供能够以优异的灵敏度检测HER2蛋白的融合蛋白，并且适用于HER2蛋白检测探针的制备。解决方案：本发明涉及一种融合蛋白，其中能够与HER2蛋白的结合融合到标记酶。

3. 发明专利

JP2014003934A Method for producing protein-nucleic acid complex and method for detecting target 有权
标题翻译：用于生产蛋白质 - 核酸复合物的方法和用于检测目标的方法
公开(公告)号：JP2014003934A
公开(公告)日：2014-01-16
申请号：JP2012141089
申请日：2012-06-22
申请人： Kyushu Univ , 国立大学法人九州大学 , Hitachi Aloka Medical Ltd , 日立アロカメディカル株式会社
发明人： KAMIYA TENHO , HAYASHI KONOSUKE , NAGAI KENJI
IPC分类号： C12P19/34 , C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6804 , C12P19/34 , C12P21/02 , G01N33/5308 , G01N33/532 , G01N33/58 , G01N33/582 , C12Q2521/119 , C12Q2521/131 , C12Q2525/205 , C12Q2563/119
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a nucleic acid probe capable of detecting a target at satisfactory sensitivity.SOLUTION: The method for producing a nucleic acid probe includes: a 3' terminal addition step of, by using a 3'terminal addition enzyme for adding a nucleotide to the 3' terminal of nucleic acid, adding at least one nucleoside triphosphate derivative having a glutamine(Gln)residue or a lysin(Lys)residue to the 3'terminal of a nucleic acid; and an indicator compound binding step of, by using transglutaminase(TGase), binding the glutamine(Gln)residue with an indicator compound having a lysin(Lys)residue and including an indicator part or binding the lysin(Lys) residue with an indicator compound having a glutamine(Gln) residue and having an indicator part.
摘要翻译：要解决的问题：提供能够以令人满意的灵敏度检测靶的核酸探针的方法。解决方案：核酸探针的制造方法包括：3'末端添加步骤，通过使用3'末端向核酸的3'末端添加核苷酸的添加酶，向核酸的3'末端添加至少一种具有谷氨酰胺（Gln）残基或赖氨酸（Lys）残基的核苷三磷酸衍生物; 和指示剂化合物结合步骤，通过使用转谷氨酰胺酶（TGase），将谷氨酰胺（Gln）残基与具有赖氨酸（Lys）残基的指示剂化合物结合并包括指示剂部分或将赖氨酸（Lys）残基与指示剂化合物具有谷氨酰胺（Gln）残基并具有指示剂部分。

4. 发明专利

JP2011169878A Nucleic acid detecting kit 有权
标题翻译：核酸检测试剂盒
公开(公告)号：JP2011169878A
公开(公告)日：2011-09-01
申请号：JP2010049631
申请日：2010-03-05
申请人： Hitachi Aloka Medical Ltd , Kyushu Univ , Univ Of Tokushima , 国立大学法人九州大学 , 国立大学法人徳島大学 , 日立アロカメディカル株式会社
发明人： KAMIYA TENHO , KITAOKA MOMOKO , TANAKA YUKARI , OGAMI YOSHIHIRO , NOJI SUMIHARU , HAYASHI KONOSUKE , MITSUMORI MASAYUKI , TOMOZOE YUSUKE
IPC分类号： G01N33/53 , C07K7/06 , C12Q1/48 , G01N33/533 , G01N33/534 , G01N33/535
摘要： PROBLEM TO BE SOLVED: To provide a nucleic acid detecting kit, capable of easily and high-sensitively detecting a target nucleic acid.
SOLUTION: The nucleic acid detecting kit includes an enzyme substrate modification nucleoside triphosphate shown in Equation 1. In Equation 1, A is a substitution group containing at least one of an alkyl group, an aminoalkyl group, an aryl group, and a heteroaryl group with linear, branched, or annular saturation, or unsaturation having a glutamine (Gln) residue or a lysine (Lys) residue, or a primary amine, and B is a hydrogen atom or a hydroxyl group.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：待解决的问题：提供能够容易且高敏感地检测靶核酸的核酸检测试剂盒。解决方案：核酸检测试剂盒包括式1中所示的酶底物修饰核苷三磷酸。在等式1中，A是含有至少一个烷基，氨基烷基，芳基和具有线性，支链或环状饱和或具有谷氨酰胺（Gln）残基或赖氨酸（Lys）残基或伯胺的不饱和基团的杂芳基，B为氢原子或羟基。版权所有（C）2011，JPO＆INPIT

5. 发明专利

JP2010132578A Nucleoside triphosphate derivative, nucleic acid probe, multi-labeled nucleic acid probe and method for detecting target nucleic acid 有权
标题翻译：核苷酸三磷酸衍生物，核酸探针，多标准核酸探针和检测目标核酸的方法
公开(公告)号：JP2010132578A
公开(公告)日：2010-06-17
申请号：JP2008307880
申请日：2008-12-02
申请人： Aloka Co Ltd , Kyushu Univ , Univ Of Tokushima , アロカ株式会社 , 国立大学法人九州大学 , 国立大学法人徳島大学
发明人： KAMIYA TENHO , TANAKA YUKARI , NOJI SUMIHARU , HAYASHI KONOSUKE , MITSUMORI MASAYUKI
IPC分类号： C07H19/10 , C07H19/20 , C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting a target nucleic acid, which simply detects the target nucleic acid in high sensitivity.
SOLUTION: The labeled nucleic acid is detected by labeled sites by using a multi-labeled nucleic acid probe obtained by previously covalently-introducing a plurality of the labeled sites to a nucleic acid probe by a reaction between an ethynyl group in the nucleic acid probe and an azide group of a labeled compound or a reaction between an azide group in the nucleic acid probe and an ethynyl group of the labeled compound, and specifically binding the multi-labeled nucleic acid probe with the target nucleic acid existing in an object by a nucleic acid site. Otherwise, the target nucleic acid is detected by labeled sites by hybridizing a target nucleic acid to be a target with a nucleic acid probe and covalently-introducing a plurality of the labeled sites to the target nucleic acid by a reaction between an ethynyl group in the nucleic acid probe and an azide group of a labeled compound or a reaction between an azide group in the nucleic acid probe and an ethynyl group of the labeled compound.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种用于检测目标核酸的方法，其以高灵敏度简单地检测靶核酸。解决方案：通过使用通过先前通过核酸中的乙炔基之间的反应将多个标记位点共价引入核酸探针而获得的多标记的核酸探针，通过标记的位点检测标记的核酸酸性探针和标记化合物的叠氮基或核酸探针中的叠氮基与标记化合物的乙炔基之间的反应，并将多标记的核酸探针与存在于物体中的靶核酸特异性结合通过核酸位点。否则，通过将靶核酸与目标核酸杂交与核酸探针，通过标记位点检测靶核酸，并通过在靶核酸中的乙炔基之间的反应将多个标记位点共价引入靶核酸核酸探针和标记化合物的叠氮基或核酸探针中的叠氮基与标记化合物的乙炔基之间的反应。版权所有（C）2010，JPO＆INPIT

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